{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:57:37Z","timestamp":1762271857985},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,11,1]],"date-time":"1984-11-01T00:00:00Z","timestamp":468115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1984,11]]},"abstract":"<jats:p>Spontaneous synaptic currents were recorded in voltage\u2010clamped CA1 neurones in rat hippocampal slices at room temperature (21\u201025 degrees C). The currents, which could be seen at the resting membrane potential only when cells were loaded with chloride ions, were blocked by bicuculline. It was concluded that they were inhibitory post\u2010synaptic currents (i.p.s.c.s) generated by the opening of chloride\u2010selective channels activated by gamma\u2010aminobutyric acid (GABA). In twenty\u2010nine cells, ten to forty\u2010five i.p.s.c.s were recorded at a potential between \u201075 and \u201085 mV. In every cell, i.p.s.c.s varied widely in amplitude with an average coefficient of variation of 28.7%. The mean amplitude varied from 0.22 to 1.16 nA with an average of 0.52 nA. Reversed (outward) currents could be recorded from cells voltage clamped at positive potentials. Null potentials varied between \u201020 and +20 mV, the variability being attributed to differences in chloride loading. Most currents had a rapid growth phase with a mean growth time (20\u201080% of peak) of 1.1 ms followed by a slower decay phase. The decay phase was exponential with a single time constant. The average decay time constant (tau D) ranged from 8.3 to 16.2 ms with a mean value of 11.0 ms. The rate of decay of currents was affected by membrane potential. tau D decreased exponentially with hyperpolarization in the range from +40 to \u2010120 mV with an average volt constant (H value) of 146 +\/\u2010 9.6 mV (mean +\/\u2010 1 S.E. of mean, n = 17). The mean value of tau D at 0 mV was 19 ms. In some cells, growth times also decreased with hyperpolarization. The decay of currents was faster at higher temperatures but remained exponential. At 32 degrees C, the average tau D at 0 mV was 8.3 ms (n = 5) giving a Q10 value of 3.3 for the decay time constant at 0 mV. The frequency and mean amplitude of i.p.s.c.s were reduced by tetrodotoxin (TTX) or cadmium, indicating that many of the currents were generated by action potentials in presynaptic terminals. The spontaneous 'miniatures' remaining had the same time course and voltage sensitivity as currents recorded in normal solutions. Pentobarbitone (50\u2010100 microM) greatly prolonged the decay of i.p.s.c.s but had no discernible effect on their amplitude or growth phase.<\/jats:p>","DOI":"10.1113\/jphysiol.1984.sp015482","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:47:57Z","timestamp":1418971677000},"page":"551-564","source":"Crossref","is-referenced-by-count":111,"title":["Inhibitory post\u2010synaptic currents in rat hippocampal CA1 neurones."],"prefix":"10.1113","volume":"356","author":[{"given":"G L","family":"Collingridge","sequence":"first","affiliation":[]},{"given":"P W","family":"Gage","sequence":"additional","affiliation":[]},{"given":"B","family":"Robertson","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1984,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1984.sp015482","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1984.sp015482","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T00:35:52Z","timestamp":1697848552000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1984.sp015482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,11]]}},"alternative-id":["10.1113\/jphysiol.1984.sp015482"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1984.sp015482","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,11]]}}}