{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:12:15Z","timestamp":1762161135144},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,2,1]],"date-time":"1990-02-01T00:00:00Z","timestamp":633830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1990,2]]},"abstract":"<jats:p>1. Bipolar cells were enzymatically (papain) dissociated from the mouse retina. Responses to exogenously applied glycine and GABA were recorded using the whole\u2010cell voltage clamp method (pipette solution contained 121 mM\u2010Cl\u2010). Both glycine and GABA evoked inward currents in cells voltage clamped at negative membrane voltages (e.g. \u201060 mV) and superfused with the control solution containing 146 mM\u2010Cl\u2010. 2. Polarities of both glycine\u2010 and GABA\u2010induced currents reversed near 0 mV under our control conditions. The reversal potential depended on both external [( Cl\u2010]o) and internal (intrapipette; [Cl\u2010]p) Cl\u2010 concentrations, but on neither Na+ nor K+ concentration. The reversal potentials were very close to the calculated equilibrium potential for Cl\u2010 estimated by using the Nernst equation with various external and internal Cl\u2010 activities. 3. The sensitivity to both glycine and GABA was highest at the axon terminal bulb. 4. Glycine\u2010induced responses were antagonized by 10 nM\u2010strychnine (competitively and non\u2010competitively), but by neither bicuculline nor picrotoxin. GABA\u2010induced responses were antagonized by 30 microM\u2010bicuculline (competitively) and 30 microM\u2010picrotoxin (non\u2010competitively), but not by 100 nM\u2010strychnine. Muscimol was as effective as GABA. Baclofen evoked no response even at 100 microM and did not modulate voltage\u2010dependent Ca2+ current. Pentobarbitone (10 microM) increased the sensitivity to GABA. These observations suggest that glycine and GABA worked on separate receptor molecules and that the receptors for GABA were GABAA type. 5. The present study suggests that glycine and GABA, both putative neurotransmitters of amacrine cells, mediate inhibition of bipolar cells in the mouse retina.<\/jats:p>","DOI":"10.1113\/jphysiol.1990.sp017967","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T14:02:50Z","timestamp":1418911370000},"page":"645-662","source":"Crossref","is-referenced-by-count":127,"title":["Effects of glycine and GABA on isolated bipolar cells of the mouse retina."],"prefix":"10.1113","volume":"421","author":[{"given":"S","family":"Suzuki","sequence":"first","affiliation":[]},{"given":"M","family":"Tachibana","sequence":"additional","affiliation":[]},{"given":"A","family":"Kaneko","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1990,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1990.sp017967","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1990.sp017967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T14:10:15Z","timestamp":1697983815000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1990.sp017967"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,2]]}},"alternative-id":["10.1113\/jphysiol.1990.sp017967"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1990.sp017967","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,2]]}}}