{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T22:44:50Z","timestamp":1773873890473,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,11,1]],"date-time":"1990-11-01T00:00:00Z","timestamp":657417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1990,11]]},"abstract":"<jats:p>1. Responses induced by intracellular cyclic nucleotides were analysed in isolated olfactory receptor cells of the newt under a voltage\u2010clamp condition by using the patch pipette in a whole\u2010cell recording configuration. Cyclic nucleotides were applied by diffusion from the patch pipette. 2. Introduction of either cyclic AMP or cyclic GMP caused a transient inward current in cells held at \u201050 mV. The response amplitude was dose\u2010dependent with the Hill coefficient of 3 and half\u2010saturating concentration of 300 microM (concentration in the pipette) for both cyclic AMP and cyclic GMP. Cyclic CMP was less effective than those two nucleotides. 3. The response to intracellular cyclic AMP was seen in all cilia\u2010bearing cells, but not in cells which lost the cilia during dissociation. The response latency was shorter when cyclic AMP was introduced into the ciliated terminal swelling (ca 0.2 s) rather than into the cell body (ca 1.4 s). These results suggest that the sensitivity to intracellular cyclic AMP is confined to the cilia. 4. The cyclic AMP\u2010induced current was transient (half decay time, ca 2.3s) despite the fact that cyclic AMP was continuously loaded from the patch pipette. The response time course was controlled by Ca2+; the reduction of external Ca2+ concentration (replaced with Mg2+) or loading the cell with 50 mM\u2010EGTA prolonged the cyclic AMP\u2010induced responses. The Ca2(+)\u2010induced suppression was reversible. 5. The reversal potential of the cyclic AMP\u2010induced transient current was \u20104.8 +\/\u2010 3.8 mV, and that of the current re\u2010induced by Ca2+ removal was 1.5 +\/\u2010 2.1 mV, suggesting that both currents flowed through the same ionic channel. The channel permeates all alkali metal ions with the permeability ratios of PLi:PNa:PK:PRb:PCs = 0.93:1:0.93:0.91:0.72, but not Cl\u2010 or choline ions. 6. These results demonstrate that the cyclic AMP\u2010induced response and the odorant\u2010induced response of the isolated olfactory cell have nearly identical characteristics. The present study supports the notion that cyclic AMP is the internal messenger mediating olfactory transduction.<\/jats:p>","DOI":"10.1113\/jphysiol.1990.sp018295","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T07:24:49Z","timestamp":1418887489000},"page":"355-371","source":"Crossref","is-referenced-by-count":99,"title":["The response induced by intracellular cyclic AMP in isolated olfactory receptor cells of the newt."],"prefix":"10.1113","volume":"430","author":[{"given":"T","family":"Kurahashi","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1990,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1990.sp018295","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1990.sp018295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T10:15:34Z","timestamp":1697969734000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1990.sp018295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,11]]}},"alternative-id":["10.1113\/jphysiol.1990.sp018295"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1990.sp018295","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11]]}}}