{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:52:21Z","timestamp":1760100741091},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1991,4,1]],"date-time":"1991-04-01T00:00:00Z","timestamp":670464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1991,4]]},"abstract":"<jats:p>1. Excitatory postsynaptic potentials (EPSPs) and their underlying currents (EPSCs) were recorded from dentate granule cells in thin hippocampal slices of rats using the tight\u2010seal whole\u2010cell recording technique. 2. At resting membrane potentials (ca \u201060 to \u201070 mV), the EPSCs clearly consisted of a dominant fast and a smaller slow component. The slow EPSC component markedly increased with depolarization. This resulted in a region of negative slope conductance (between \u201050 and \u201030 mV) in the peak current\u2010voltage (I\u2010V) relation of the dual\u2010component EPSC in most neurones. The EPSCs reversed entirely at \u20101.2 +\/\u2010 2.8 mV (n = 15). 3. Using selective antagonists of N\u2010methyl\u2010D\u2010aspartate (NMDA) and non\u2010NMDA excitatory amino acid receptors, two pharmacologically distinct components of the natural EPSCs were isolated. The non\u2010NMDA EPSCs displayed a linear I\u2010V relation. Their rise times (0.5\u20101.9 ms) were independent of membrane voltage but seemed to depend critically on the precise dendritic location of the synapse. Their decay was approximated by a single exponential with a time constant ranging from 3 to 9 ms. The time course of these EPSCs was independent of changes in extracellular Mg2+. 4. The NMDA EPSCs displayed a non\u2010linear I\u2010V relation. At resting membrane potentials their peak amplitudes were 20 pA and increased steadily with depolarization to \u201030 mV. At membrane voltages positive to \u201030 mV the peak I\u2010V relation was linear. The rise times of NMDA EPSCs ranged from 4 to 9 ms and were insensitive to membrane voltage. 5. The NMDA EPSCs decayed biexponentially. Both time constants, tau f and tau s, increased with depolarization in an exponential manner, tau s being more voltage dependent than tau f. Lowering extracellular Mg2+ slightly reduced both rate constants but did not completely abolish their voltage sensitivity. 6. Bath application of NMDA to outside\u2010out patches from granule cells induced single channel currents of 52 pS in nominally Mg(2+)\u2010free solutions. They displayed a burst\u2010like single\u2010channel activity with clusters of bursts lasting several hundreds of milliseconds. Currents through single NMDA receptor channels reversed around 0 mV. 7. The fractional contributions of NMDA and non\u2010NMDA components to peak currents and synaptic charge transfer were assessed. At resting membrane potential the NMDA EPSC component accounted for 23% of the peak current and for 64% of the synaptic charge transfer. The contribution of the NMDA EPSC component to the synaptic charge transfer strongly increased with small depolarizations from rest.<\/jats:p>","DOI":"10.1113\/jphysiol.1991.sp018510","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T23:18:54Z","timestamp":1418858334000},"page":"275-293","source":"Crossref","is-referenced-by-count":101,"title":["Patch clamp analysis of excitatory synaptic currents in granule cells of rat hippocampus."],"prefix":"10.1113","volume":"435","author":[{"given":"B U","family":"Keller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Konnerth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Yaari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1991.sp018510","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1991.sp018510","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T04:58:39Z","timestamp":1698037119000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1991.sp018510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,4]]}},"alternative-id":["10.1113\/jphysiol.1991.sp018510"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018510","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,4]]}}}