{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:54:18Z","timestamp":1772758458951,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1995,6,1]],"date-time":"1995-06-01T00:00:00Z","timestamp":801964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:p>1. The beta\u2010subunit has marked effects on the biophysical and pharmacological properties of voltage\u2010dependent calcium channels. In the present study we examined the ability of the GABAB agonist (\u2010) \u2010baclofen to inhibit calcium channel currents in cultured rat dorsal root ganglion neurones following depletion of beta\u2010subunit immunoreactivity, 108\u2010116 h after microinjection of a beta\u2010subunit antisense oligonucleotide. 2.We observed that, although the calcium channel current was markedly reduced in amplitude following beta\u2010subunit depletion, the residual current (comprising both N\u2010 and L\u2010type calcium channel currents) showed an enhanced response to application of (\u2010) \u2010baclofen. Therefore, it is possible that there is normally competition between activated G protein G(o) and the calcium channel beta\u2010subunit for binding to the calcium channel alpha 1\u2010subunit; and this competition shifts in favour of the binding of activated G(o) following depletion of the beta\u2010subunit, resulting in increased inhibition. 3. This hypothesis is supported by evidence that an antibody against the calcium channel beta\u2010subunit completely abolishes stimulation of the GTPase activity of G(o) by the dihydropyridine agonist S\u2010(\u2010) \u2010Bay K 8644 in brain membranes. This stimulation of GTPase is thought to result from an interaction of G(o) alpha\u2010subunit (G alpha o) with its calcium channel effector which may operate as a GTPase\u2010activating protein. 4. These data suggest that the calcium channel beta\u2010subunit when complexed with the beta 1\u2010subunit normally inhibits its association with activated G(o). It may function as a GTPase\u2010activating protein to reduce the ability of activated G(o) to associate with the calcium channel, and thus limit the efficacy of agonists such as (\u2010) \u2010baclofen.<\/jats:p>","DOI":"10.1113\/jphysiol.1995.sp020735","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T10:30:42Z","timestamp":1418812242000},"page":"365-372","source":"Crossref","is-referenced-by-count":101,"title":["Inhibition of the interaction of G protein G(o) with calcium channels by the calcium channel beta\u2010subunit in rat neurones."],"prefix":"10.1113","volume":"485","author":[{"given":"V","family":"Campbell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N S","family":"Berrow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E M","family":"Fitzgerald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Brickley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A C","family":"Dolphin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1995.sp020735","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1995.sp020735","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T17:01:38Z","timestamp":1698339698000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1995.sp020735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,6]]}},"alternative-id":["10.1113\/jphysiol.1995.sp020735"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1995.sp020735","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,6]]}}}