{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T21:15:48Z","timestamp":1767993348215,"version":"3.49.0"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T00:00:00Z","timestamp":1741478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Obesity is associated with insulin resistance (IR) development, a risk factor for type 2 diabetes (T2D). How mitochondrial bioenergetics, in adipose tissue (AT), differs according to distinct metabolic profiles (i.e. insulin sensitive (IS), IR normoglycaemic (IR\u2010NG), pre\u2010diabetes (PD) and T2D) is still poorly understood. The purpose of this study was to evaluate and compare bioenergetics and energy substrate preference by omental AT (OAT) and subcutaneous AT (SAT) from subjects with obesity (OB, <jats:italic>n<\/jats:italic> = 40) at distinct metabolic stages. Furthermore, AT bioenergetics was also evaluated pre\u2010 and post\u2010bariatric\/metabolic surgery (BMS). High\u2010resolution respirometry (HRR) was used to measure the real\u2010time oxidative phosphorylation (OXPHOS) capacity and mitochondrial substrate preferences in both tissues. Substrate\u2010uncoupler\u2010inhibitor titration protocols were used: SUIT\u2010P1 (complex I and II\u2010linked mitochondrial respiration) and SUIT\u2010P2 (fatty acid oxidation (FAO)\u2010linked mitochondrial respiration). Flux control ratios (FCRs) were calculated. In SUIT\u2010P1, lower OXPHOS capacity was observed in AT, particularly in SAT, during the establishment of IR (OB\u2010IR\u2010NG) and in the T2D group, due to alterations of mitochondrial coupling, evaluated by FCRs. In SUIT\u2010P2, the OXPHOS coupling efficiency was highest in the OB\u2010IR\u2010NG group. AT from OB\u2010IS, OB\u2010IR\u2010NG and OB\u2010IR\u2010PD preferred pyruvate, malate and glutamate oxidation and\/or FAO during OXPHOS, whereas AT from T2D preferred succinate oxidation. BMS enhanced mitochondrial respiration in OAT, even under poor OXPHOS coupling efficiency. In conclusion, real\u2010time OXPHOS analysis by HRR may be a sensitive biomarker of mitochondrial fitness, particularly in AT. Interventions based on modulating energetic substrate availability may become a good tool for obesity treatment stratification.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16617-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Omental adipose tissue shows higher oxidative phosphorylation (OXPHOS) capacity compared to subcutaneous adipose tissue in paired explants from subjects with obesity.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The OXPHOS capacity of adipose tissue differs through the progression of metabolic disease. Subjects with obesity and diabetes have the lowest OXPHOS capacity in paired explants of subcutaneous and omental adipose tissues.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Bariatric surgery enhanced the OXPHOS capacity in omental adipose tissue, even under poor OXPHOS coupling efficiency.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Assessment of the oxidative capacity in fresh adipose tissue explants could be a sensitive tool for early diagnosis of metabolic disease.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp286103","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:17:30Z","timestamp":1741753050000},"page":"2583-2617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of the metabolic disease status in obesity and surgical weight loss on human adipose tissue bioenergetics"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2235-2237","authenticated-orcid":false,"given":"Aryane Cruz Oliveira","family":"Pinho","sequence":"first","affiliation":[{"name":"CNC UC \u2013 Center for Neuroscience and Cell Biology University of Coimbra  Coimbra Portugal"},{"name":"CIBB \u2013 Center for Innovative Biomedicine and Biotechnology University of Coimbra  Coimbra Portugal"},{"name":"Department of Life Sciences, Faculty of Science and Technology University of Coimbra  Coimbra Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3910-845X","authenticated-orcid":false,"given":"Andr\u00e9","family":"Lazaro","sequence":"additional","affiliation":[{"name":"General Surgery Unit, Centro Hospitalar e Universit\u00e1rio de Coimbra University of Coimbra  Coimbra Portugal"},{"name":"Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine University of Coimbra  Coimbra 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