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Adapting the micronucleus assay from filter plates to V-shaped plates presented challenges in the liquid handling, namely, cell splashing out of the V-shaped well plate during the cell harvesting, poor cell distribution on the bottom of the image plate during the dispensing, and cell loss from the image plate during the aspiration in the liquid handling process. Experimental and numerical investigations were carried out to better understand the phenomena and mitigate the problems. Surface tension and contact angle among the fluids and the plate wall were accounted for in the discrete and multiphase numerical models. Experimental conditions were optimized based on the numerical results showing the relationship between nozzle speed and amount of splashed liquid, and the relationship between aspiration speed and number of escaped cells. Using these optimized parameters, numbers of micronuclei in binucleated cells showed the same dose dependence in the RABiT-prepared samples as those in the manually prepared ones. Micronucleus assay protocol was fully realized on RABiT.<\/jats:p>","DOI":"10.1115\/1.4033600","type":"journal-article","created":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T16:34:33Z","timestamp":1463157273000},"update-policy":"https:\/\/doi.org\/10.1115\/crossmarkpolicy-asme","source":"Crossref","is-referenced-by-count":6,"title":["Liquid Handling Optimization in High-Throughput Biodosimetry Tool"],"prefix":"10.1115","volume":"10","author":[{"given":"Dakai","family":"Bian","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, Columbia University, New York, NY 10027 e-mail:"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason C.","family":"Tsui","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Columbia University, New York, NY 10027"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikhail","family":"Repin","sequence":"additional","affiliation":[{"name":"Center for Radiological Research, Columbia University Medical Center, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Garty","sequence":"additional","affiliation":[{"name":"Center for Radiological Research, Columbia University Medical Center, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Turner","sequence":"additional","affiliation":[{"name":"Center for Radiological Research, Columbia University Medical Center, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Lawrence Yao","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Columbia University, New York, NY 10027"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Brenner","sequence":"additional","affiliation":[{"name":"Center for Radiological Research, Columbia University Medical Center, New York, NY 10032"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"33","published-online":{"date-parts":[[2016,8,31]]},"reference":[{"issue":"9","key":"2019100618205673600_bib1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-6-9","article-title":"High-Throughput Isolation of Ultra-Pure Plasmid DNA by a Robotic System","volume":"6","year":"2006","journal-title":"BMC Biotechnol."},{"key":"2019100618205673600_bib2","doi-asserted-by":"crossref","unstructured":"Meldrum, D. 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