{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:53:53Z","timestamp":1790790833272,"version":"4.1.0"},"reference-count":98,"publisher":"ASME International","issue":"4","funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R15AI127214"],"award-info":[{"award-number":["R15AI127214"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006827","name":"Florida Department of Health","doi-asserted-by":"publisher","award":["7ZK10"],"award-info":[{"award-number":["7ZK10"]}],"id":[{"id":"10.13039\/100006827","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asmedigitalcollection.asme.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:p>The Zika virus (ZIKV) is one of the most infamous mosquito-borne flavivirus on recent memory due to its potential association with high mortality rates in fetuses, microcephaly and neurological impairments in neonates, and autoimmune disorders. The severity of the disease, as well as its fast spread over several continents, has urged the World Health Organization (WHO) to declare ZIKV a global health concern. In consequence, over the past couple of years, there has been a significant effort for the development of ZIKV diagnostic methods, vaccine development, and prevention strategies. This review focuses on the most recent aspects of ZIKV research which includes the outbreaks, genome structure, multiplication and propagation of the virus, and more importantly, the development of serological and molecular detection tools such as Zika IgM antibody capture enzyme-linked immunosorbent assay (Zika MAC-ELISA), plaque reduction neutralization test (PRNT), reverse transcription quantitative real-time polymerase chain reaction (qRT-PCR), reverse transcription-loop mediated isothermal amplification (RT-LAMP), localized surface plasmon resonance (LSPR) biosensors, nucleic acid sequence-based amplification (NASBA), and recombinase polymerase amplification (RPA). Additionally, we discuss the limitations of currently available diagnostic methods, the potential of newly developed sensing technologies, and also provide insight into future areas of research.<\/jats:p>","DOI":"10.1115\/1.4041086","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T16:50:33Z","timestamp":1533833433000},"update-policy":"https:\/\/doi.org\/10.1115\/crossmarkpolicy-asme","source":"Crossref","is-referenced-by-count":37,"title":["Advances in Diagnostic Methods for Zika Virus Infection"],"prefix":"10.1115","volume":"12","author":[{"given":"Carlos A.","family":"Herrada","sequence":"first","affiliation":[{"name":"Department of Computer Engineering and Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431;"},{"name":"Asghar-Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Boca Raton, FL 33431"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Alamgir","family":"Kabir","sequence":"additional","affiliation":[{"name":"Department of Computer Engineering and Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431;"},{"name":"Asghar-Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Boca Raton, FL 33431"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rommel","family":"Altamirano","sequence":"additional","affiliation":[{"name":"Department of Computer Engineering and Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431;"},{"name":"Asghar-Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Boca Raton, FL 33431"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waseem","family":"Asghar","sequence":"additional","affiliation":[{"name":"Department of Computer Engineering and Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431;"},{"name":"Asghar-Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Boca Raton, FL 33431;"},{"name":"Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL 33431 e-mail:"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"33","published-online":{"date-parts":[[2018,11,5]]},"reference":[{"key":"2019100618285986600_bib1","article-title":"Rapid Risk Assessment: Zika Virus Epidemic in the Americas: Potential Association With Microcephaly and Guillain-Barr\u00e9 Syndrome","author":"ECDC","year":"2015"},{"key":"2019100618285986600_bib2","article-title":"Facts About Microcephaly","author":"U.S. CDC,","year":"2018"},{"issue":"3","key":"2019100618285986600_bib3","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1128\/CMR.00014-16","article-title":"Emerging Role of Zika Virus in Adverse Fetal and Neonatal Outcomes","volume":"29","year":"2016","journal-title":"Clin. Microbiol. Rev."},{"issue":"5","key":"2019100618285986600_bib4","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.stem.2016.02.016","article-title":"Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth","volume":"18","year":"2016","journal-title":"Cell Stem Cell"},{"issue":"6283","key":"2019100618285986600_bib5","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1126\/science.aaf5036","article-title":"Zika Virus in the Americas: Early Epidemiological and Genetic Findings","volume":"352","year":"2016","journal-title":"Science"},{"issue":"24","key":"2019100618285986600_bib6","doi-asserted-by":"publisher","first-page":"2321","DOI":"10.1056\/NEJMoa1602412","article-title":"Zika Virus Infection in Pregnant Women in Rio De Janeiro","volume":"375","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"2019100618285986600_bib7","doi-asserted-by":"publisher","article-title":"Zika Virus Disease in Colombia\u2014Preliminary Report","journal-title":"New Engl. J. Med.","DOI":"10.1056\/NEJMoa1604037"},{"issue":"3","key":"2019100618285986600_bib8","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1128\/CMR.00072-15","article-title":"Zika Virus","volume":"29","year":"2016","journal-title":"Clin. Microbiol. Rev."},{"key":"2019100618285986600_bib9","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.142","article-title":"Guillain-Barre Syndrome, Transverse Myelitis and Infectious Diseases","volume":"15","year":"2018","journal-title":"Cell Mol. Immunol."},{"issue":"6","key":"2019100618285986600_bib10","doi-asserted-by":"publisher","first-page":"597","DOI":"10.2165\/00003495-200464060-00003","article-title":"Guillain-Barre Syndrome: Epidemiology, Pathophysiology and Management","volume":"64","year":"2004","journal-title":"Drugs"},{"issue":"8","key":"2019100618285986600_bib11","doi-asserted-by":"publisher","first-page":"2122","DOI":"10.1093\/brain\/aww158","article-title":"Zika Virus-Associated Neurological Disorders: A Review","volume":"139","year":"2016","journal-title":"Brain"},{"key":"2019100618285986600_bib12","article-title":"WHO Statement on the First Meeting of the International Health Regulations (2005) (IHR 2005) Emergency Committee on Zika Virus and Observed Increase in Neurological Disorders and Neonatal Malformations","author":"WHO","year":"2016"},{"issue":"1","key":"2019100618285986600_bib13","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.tmaid.2015.07.005","article-title":"A Bibliometric Analysis of Global Zika Research","volume":"14","year":"2016","journal-title":"Travel Med. Infect. Dis."},{"issue":"2","key":"2019100618285986600_bib14","doi-asserted-by":"publisher","first-page":"e000087","DOI":"10.1136\/bmjgh-2016-000087","article-title":"Epidemiology of Zika Virus, 1947-2007","volume":"1","year":"2016","journal-title":"BMJ Glob. Health"},{"issue":"7","key":"2019100618285986600_bib15","doi-asserted-by":"publisher","first-page":"e119","DOI":"10.1016\/S1473-3099(16)30010-X","article-title":"Zika Virus: History of a Newly Emerging Arbovirus","volume":"16","year":"2016","journal-title":"Lancet Infect. Dis."},{"issue":"24","key":"2019100618285986600_bib16","doi-asserted-by":"publisher","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika Virus Outbreak on Yap Island, Federated States of Micronesia","volume":"360","year":"2009","journal-title":"N. Engl. J. Med."},{"issue":"10","key":"2019100618285986600_bib17","doi-asserted-by":"publisher","first-page":"O595","DOI":"10.1111\/1469-0691.12707","article-title":"Rapid Spread of Emerging Zika Virus in the Pacific Area","volume":"20","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"issue":"10107","key":"2019100618285986600_bib18","doi-asserted-by":"publisher","first-page":"2099","DOI":"10.1016\/S0140-6736(17)31450-2","article-title":"An Update on Zika Virus Infection","volume":"390","year":"2017","journal-title":"Lancet"},{"issue":"5","key":"2019100618285986600_bib19","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1590\/0037-8682-0328-2016","article-title":"One Year After the Zika Virus Outbreak in Brazil: From Hypotheses to Evidence","volume":"49","year":"2016","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"2019100618285986600_bib20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jaut.2016.02.006","article-title":"The Zika Outbreak of the 21st Century","volume":"68","year":"2016","journal-title":"J. Autoimmun."},{"issue":"7","key":"2019100618285986600_bib21","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/j.apjtm.2016.05.010","article-title":"Zika Virus Infections: An Overview of Current Scenario","volume":"9","year":"2016","journal-title":"Asian Pac. J. Trop. Med."},{"issue":"9","key":"2019100618285986600_bib22","doi-asserted-by":"publisher","first-page":"675","DOI":"10.2471\/BLT.16.171082","article-title":"Zika: The Origin and Spread of a Mosquito-Borne Virus","volume":"94","year":"2016","journal-title":"Bull. World Health Organ."},{"issue":"13","key":"2019100618285986600_bib23","doi-asserted-by":"publisher","first-page":"20751","DOI":"10.2807\/1560-7917.ES2014.19.13.20751","article-title":"Evidence of Perinatal Transmission of Zika Virus, French Polynesia, December 2013 and February 2014","volume":"19","year":"2014","journal-title":"Eurosurveillance"},{"issue":"5","key":"2019100618285986600_bib24","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1136\/sextrans-2015-052189","article-title":"Zika: Another Sexually Transmitted Infection?","volume":"91","year":"2015","journal-title":"Sex Transm. Infect."},{"key":"2019100618285986600_bib25","doi-asserted-by":"publisher","first-page":"110","DOI":"10.3389\/fmicb.2017.00110","article-title":"Zika Virus: Transmission, Detection, Control, and Prevention","volume":"8","year":"2017","journal-title":"Front. Microbiol."},{"issue":"13","key":"2019100618285986600_bib26","article-title":"Evidence of Perinatal Transmission of Zika Virus, French","volume":"19","year":"2014","journal-title":"Euro Surveill."},{"issue":"1","key":"2019100618285986600_bib27","doi-asserted-by":"publisher","first-page":"69","DOI":"10.3390\/v6010069","article-title":"Flavivirus Entry Receptors: An Update","volume":"6","year":"2013","journal-title":"Viruses"},{"key":"2019100618285986600_bib28","doi-asserted-by":"publisher","first-page":"496","DOI":"10.3389\/fmicb.2016.00496","article-title":"Zika Virus: The Latest Newcomer","volume":"7","year":"2016","journal-title":"Front. Microbiol."},{"key":"2019100618285986600_bib29","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00055-16","article-title":"The Antigenic Structure of Zika Virus and Its Relation to Other Flaviviruses: Implications for Infection and Immunoprophylaxis","volume":"81","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"2019100618285986600_bib30","article-title":"Zika Virus: The Emerging Global Health Challenge","volume":"13","journal-title":"Diversity and Equality in Health and Care"},{"issue":"8","key":"2019100618285986600_bib31","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and Serologic Properties of Zika Virus Associated With an Epidemic, Yap State, Micronesia, 2007","volume":"14","year":"2008","journal-title":"Emerg. Infect. Dis."},{"issue":"9","key":"2019100618285986600_bib32","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1002\/jmv.24735","article-title":"Molecular Detection of Zika Virus in Blood and RNA Load Determination During the French Polynesian Outbreak","volume":"89","year":"2017","journal-title":"J. Med. Virol."},{"issue":"6","key":"2019100618285986600_bib33","doi-asserted-by":"publisher","first-page":"e0004816","DOI":"10.1371\/journal.pntd.0004816","article-title":"Isolation of Infective Zika Virus From Urine and Saliva of Patients in Brazil","volume":"10","year":"2016","journal-title":"PLOS Neglected Trop. Dis."},{"issue":"3","key":"2019100618285986600_bib34","doi-asserted-by":"publisher","first-page":"e731737","DOI":"10.1016\/j.urology.2014.04.048","article-title":"The Influence of Urinary pH on Antibiotic Efficacy Against Bacterial Uropathogens","volume":"84","year":"2014","journal-title":"Urology"},{"issue":"4","key":"2019100618285986600_bib35","doi-asserted-by":"publisher","first-page":"461","DOI":"10.4103\/0972-124X.118317","article-title":"Salivary pH: A Diagnostic Biomarker","volume":"17","year":"2013","journal-title":"J. Indian Soc. Periodontol."},{"issue":"Pt. 10","key":"2019100618285986600_bib36","doi-asserted-by":"publisher","first-page":"2755","DOI":"10.1099\/vir.0.82210-0","article-title":"Flavivirus Membrane Fusion","volume":"87","year":"2006","journal-title":"J. Gen. Virol."},{"issue":"5","key":"2019100618285986600_bib37","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1097\/QCO.0000000000000301","article-title":"Zika Virus Infection: Epidemiology, Clinical Manifestations and Diagnosis","volume":"29","year":"2016","journal-title":"Curr. Opin. Infect. Dis."},{"key":"2019100618285986600_bib38","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.ebiom.2017.03.006","article-title":"A Rapid Zika Diagnostic Assay to Measure Neutralizing Antibodies in Patients","volume":"17","year":"2017","journal-title":"EBioMedicine"},{"key":"2019100618285986600_bib39","article-title":"Aptima Zika Virus Assay","author":"Hologic","year":"2018"},{"key":"2019100618285986600_bib40","article-title":"Gene-RADAR Zika Virus Test Instructions for Use","author":"Nanobiosym Diagnostics","year":"2017"},{"key":"2019100618285986600_bib41","article-title":"RealStar\u00ae Zika Virus RT-PCR Kit U.S. Instructions for Use","author":"Altona Diagnostics","year":"2017"},{"key":"2019100618285986600_bib42","article-title":"Real Time Zika Instructions for Use","author":"Abbott","year":"2016"},{"key":"2019100618285986600_bib43","article-title":"Sentosa\u00ae SA ZIKV RT-PCR Test Instructions for Use","author":"Vela Diagnostics","year":"2016"},{"key":"2019100618285986600_bib44","article-title":"TaqPath Zika Virus Kit Instructions for Use","author":"Thermo Fisher Scientific","year":"2017"},{"key":"2019100618285986600_bib45","article-title":"Trioplex Real-Time RT-PCR Assay","author":"CDC","year":"2017"},{"key":"2019100618285986600_bib46","article-title":"VERSANT Zika RNA 1.0 Assay Kit","author":"Siemens","year":"2016"},{"key":"2019100618285986600_bib47","article-title":"xMAP\u00ae MultiFLEX\u2122 Zika RNA Assay Instructions for Use","author":"Luminex","year":"2017"},{"key":"2019100618285986600_bib48","article-title":"Zika Virus Detection by RT-PCR Test Instructions for Use","author":"ARUP Laboratories","year":"2016"},{"key":"2019100618285986600_bib49","article-title":"Zika Virus Real-Time RT-PCR Test Instructions for Use","author":"Viracor Eurofins","year":"2017"},{"key":"2019100618285986600_bib50","article-title":"Zika Virus RNA Qualitative Real-Time RT-PCR Instructions for Use","author":"Quest Diagnostics","year":"2017"},{"key":"2019100618285986600_bib51","article-title":"Zika ELITe MGB Kit Instructions for Use","author":"ELITechGroup,","year":"2016"},{"key":"2019100618285986600_bib52","article-title":"ADVIA Centaur Zika Test Instructions for Use","author":"Siemens Diagnostics","year":"2017"},{"key":"2019100618285986600_bib53","article-title":"DPP Zika IgM Assay System Instructions for Use","author":"Chembio Diagnostic","year":"2018"},{"key":"2019100618285986600_bib54","article-title":"LIAISON XL Zika Capture IgM Assay Instructions for Use","author":"DiaSorin,","year":"2017"},{"key":"2019100618285986600_bib55","article-title":"Zika MAC-ELISA Instructions for Use","author":"CDC","year":"2017"},{"key":"2019100618285986600_bib56","article-title":"ZIKV Detect IgM Capture ELISA Instructions for Use","author":"InBios International","year":"2017"},{"key":"2019100618285986600_bib57","article-title":"Types of Zika Virus Test","author":"U.S. CDC","year":"2017"},{"key":"2019100618285986600_bib58","article-title":"Trioplex Real-Time RT-PCR Assay","author":"U.S. CDC","year":"2017"},{"issue":"6","key":"2019100618285986600_bib59","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1080\/1040841X.2017.1313814","article-title":"Strategies in Ebola Virus Disease (EVD) Diagnostics at the Point of Care","volume":"43","year":"2017","journal-title":"Crit. Rev. Microbiol."},{"issue":"4","key":"2019100618285986600_bib60","doi-asserted-by":"publisher","first-page":"441","DOI":"10.3109\/07388551.2016.1167667","article-title":"Advances in Candida Detection Platforms for Clinical and Point-of-Care Applications","volume":"37","year":"2017","journal-title":"Crit. Rev. Biotechnol."},{"issue":"8","key":"2019100618285986600_bib61","doi-asserted-by":"publisher","first-page":"574","DOI":"10.2471\/BLT.16.171207","article-title":"Background Review for Diagnostic Test Development for Zika Virus Infection","volume":"94","year":"2016","journal-title":"Bull World Health Organ."},{"key":"2019100618285986600_bib62","article-title":"Zika Mac-ELISA Instructions for Use","author":"U.S FDA","year":"2018"},{"issue":"28","key":"2019100618285986600_bib63","doi-asserted-by":"publisher","first-page":"7852","DOI":"10.1073\/pnas.1607931113","article-title":"Human Antibody Responses After Dengue Virus Infection Are Highly Cross-Reactive to Zika Virus","volume":"113","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2019100618285986600_bib64","first-page":"7","article-title":"Future Developments in Biosensors for Field-Ready Zika Virus Diagnostics","volume-title":"J. Biol. Eng","year":"2017"},{"issue":"3","key":"2019100618285986600_bib65","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01591-17","article-title":"Development of Zika Virus Serological Testing Strategies in New York State","volume":"56","year":"2018","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"2019100618285986600_bib66","doi-asserted-by":"publisher","first-page":"3028","DOI":"10.1128\/JCM.00975-17","article-title":"Evaluation of a Novel Reporter Virus Neutralization Test for Serological Diagnosis of Zika and Dengue Virus Infection","volume":"55","year":"2017","journal-title":"J. Clin. Microbiol."},{"issue":"20","key":"2019100618285986600_bib67","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00834-17","article-title":"Development of Virus-Like-Particle Vaccine and Reporter Assay for Zika Virus","volume":"91","year":"2017","journal-title":"J. Virol."},{"key":"2019100618285986600_bib68","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.ebiom.2017.01.008","article-title":"A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis","volume":"16","year":"2017","journal-title":"EBioMedicine"},{"issue":"1","key":"2019100618285986600_bib69","doi-asserted-by":"publisher","first-page":"8719","DOI":"10.1038\/srep08719","article-title":"Paper and Flexible Substrates as Materials for Biosensing Platforms to Detect Multiple Biotargets","volume":"5","year":"2015","journal-title":"Sci. Rep."},{"issue":"4","key":"2019100618285986600_bib70","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1080\/14737159.2017.1285228","article-title":"Based Analytical Devices for Clinical Diagnosis: Recent Advances in the Fabrication Techniques and Sensing Mechanisms","volume":"17","year":"2017","journal-title":"Expert Rev. Mol. Diagn."},{"issue":"2","key":"2019100618285986600_bib71","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1007\/s00216-008-2287-2","article-title":"Lateral Flow (Immuno) Assay: Its Strengths, Weaknesses, Opportunities and Threats. A Literature Survey","volume":"393","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"key":"2019100618285986600_bib72","volume-title":"Diagnostics for the Developing World: Microfluidic Paper-Based Analytical Devices","year":"2009"},{"issue":"9","key":"2019100618285986600_bib73","doi-asserted-by":"publisher","first-page":"1520","DOI":"10.1002\/jmv.24806","article-title":"Paper-Based Point-of-Care Testing for Cost-Effective Diagnosis of Acute Flavivirus Infections","volume":"89","year":"2017","journal-title":"J. Med. Virol."},{"issue":"26","key":"2019100618285986600_bib74","doi-asserted-by":"publisher","first-page":"1707161","DOI":"10.1002\/adfm.201707161","article-title":"Hybrid Paper\u2013Plastic Microchip for Flexible and High\u2010Performance Point\u2010of\u2010Care Diagnostics","volume":"28","year":"2018","journal-title":"Adv. Funct. Mater."},{"issue":"8","key":"2019100618285986600_bib75","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1002\/anie.200603817","article-title":"Patterned Paper as a Platform for Inexpensive, Low-Volume, Portable Bioassays","volume":"46","year":"2007","journal-title":"Angew. Chem. Int. Ed. Engl."},{"issue":"11","key":"2019100618285986600_bib76","doi-asserted-by":"publisher","first-page":"5728","DOI":"10.1021\/cr4000623","article-title":"Advances in Plasmonic Technologies for Point of Care Applications","volume":"114","year":"2014","journal-title":"Chem. Rev."},{"key":"2019100618285986600_bib77","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1016\/j.bios.2017.03.046","article-title":"Localized Surface Plasmon Resonance-Mediated Fluorescence Signals in Plasmonic Nanoparticle-Quantum Dot Hybrids for Ultrasensitive Zika Virus RNA Detection Via Hairpin Hybridization Assays","volume":"94","year":"2017","journal-title":"Biosens. Bioelectron."},{"issue":"5","key":"2019100618285986600_bib78","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1016\/j.cell.2016.04.059","article-title":"Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components","volume":"165","year":"2016","journal-title":"Cell"},{"issue":"3","key":"2019100618285986600_bib79","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1021\/acsbiomaterials.5b00449","article-title":"Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection","volume":"2","year":"2016","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"3","key":"2019100618285986600_bib80","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1373\/clinchem.2016.263665","article-title":"Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection","volume":"63","year":"2017","journal-title":"Clin. Chem."},{"issue":"14","key":"2019100618285986600_bib81","doi-asserted-by":"publisher","first-page":"7289","DOI":"10.1021\/acs.analchem.6b01632","article-title":"Instrument-Free Point-of-Care Molecular Detection of Zika Virus","volume":"88","year":"2016","journal-title":"Anal. Chem."},{"issue":"388","key":"2019100618285986600_bib82","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aag0538","article-title":"Rapid and Specific Detection of Asian- and African-Lineage Zika Viruses","volume":"9","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"2019100618285986600_bib83","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.jviromet.2016.10.010","article-title":"Rapid and Sensitive Detection of Zika Virus by Reverse Transcription Loop-Mediated Isothermal Amplification","volume":"238","year":"2016","journal-title":"J. Virol. Methods"},{"key":"2019100618285986600_bib84","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.mex.2014.08.009","article-title":"New Closed Tube Loop Mediated Isothermal Amplification Assay for Prevention of Product Cross-Contamination","volume":"1","year":"2014","journal-title":"MethodsX"},{"key":"2019100618285986600_bib85","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.ab.2018.01.008","article-title":"Moving Toward Rapid and Low-Cost Point-of-Care Molecular Diagnostics With a Repurposed 3D Printer and RPA","volume":"545","year":"2018","journal-title":"Anal. Biochem."},{"issue":"1","key":"2019100618285986600_bib86","doi-asserted-by":"publisher","first-page":"38223","DOI":"10.1038\/srep38223","article-title":"Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus","volume":"6","year":"2016","journal-title":"Sci. Rep."},{"key":"2019100618285986600_bib87","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.03.007","article-title":"Circulating Tumor Cell Isolation, Culture, and Downstream Molecular Analysis","volume":"36","year":"2018","journal-title":"Biotechnol. Adv."},{"issue":"1","key":"2019100618285986600_bib88","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1111\/andr.12441","article-title":"Emerging Technologies for Home\u2010Based Semen Analysis","volume":"6","year":"2018","journal-title":"Andrology"},{"issue":"4","key":"2019100618285986600_bib89","first-page":"10","article-title":"Image Sensor Road Map and Solid-State Imaging Devices","volume":"1","year":"2017","journal-title":"NanoWorld"},{"issue":"17","key":"2019100618285986600_bib90","doi-asserted-by":"publisher","first-page":"2910","DOI":"10.1039\/C7LC00273D","article-title":"Rapid, Label-Free CD4 Testing Using a Smartphone Compatible Device","volume":"17","year":"2017","journal-title":"Lab Chip"},{"key":"2019100618285986600_bib91","doi-asserted-by":"publisher","DOI":"10.9734\/BJMMR\/2014\/7392","article-title":"Cell Elasticity-Based Microfluidic Label-Free Isolation of Metastatic Tumor Cells","volume-title":"British Journal of Medicine and Medical Research","year":"2014"},{"issue":"18","key":"2019100618285986600_bib92","doi-asserted-by":"publisher","first-page":"7166","DOI":"10.1039\/C4AY00781F","article-title":"Electrophysiological Analysis of Biopsy Samples Using Elasticity as an Inherent Cell Marker for Cancer Detection","volume":"6","year":"2014","journal-title":"Anal. Methods"},{"issue":"1","key":"2019100618285986600_bib93","doi-asserted-by":"publisher","first-page":"21163","DOI":"10.1038\/srep21163","article-title":"Engineering Long Shelf Life Multi-Layer Biologically Active Surfaces on Microfluidic Devices for Point of Care Applications","volume":"6","year":"2016","journal-title":"Sci. Rep."},{"issue":"3","key":"2019100618285986600_bib94","doi-asserted-by":"publisher","first-page":"030911","DOI":"10.1115\/1.4001664","article-title":"Rapid Nanomanufacturing of Metallic Break Junctions Using Focused Ion Beam Scratching and Electromigration","volume":"132","year":"2010","journal-title":"ASME J. Manuf. Sci. Eng."},{"key":"2019100618285986600_bib95","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/j.bios.2014.06.048","article-title":"Parallel Recognition of Cancer Cells Using an Addressable Array of Solid-State Micropores","volume":"62","year":"2014","journal-title":"Biosens. Bioelectron."},{"issue":"6","key":"2019100618285986600_bib96","doi-asserted-by":"publisher","first-page":"2110","DOI":"10.1073\/pnas.1323998111","article-title":"Digital Microfluidic Assay for Protein Detection","volume":"111","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"6","key":"2019100618285986600_bib97","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1007\/s10544-015-0022-2","article-title":"PicoMolar Level Detection of Protein Biomarkers Based on Electronic Sizing of Bead Aggregates: Theoretical and Experimental Considerations","volume":"17","year":"2015","journal-title":"Biomed Microdev."},{"issue":"10","key":"2019100618285986600_bib98","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1039\/C8LC00033F","article-title":"A Microfluidic Biochip Platform for Electrical Quantification of Proteins","volume":"18","year":"2018","journal-title":"Lab Chip"}],"container-title":["Journal of Medical Devices"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/asmedigitalcollection.asme.org\/medicaldevices\/article-pdf\/doi\/10.1115\/1.4041086\/6241115\/med_012_04_040802.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/asmedigitalcollection.asme.org\/medicaldevices\/article-pdf\/doi\/10.1115\/1.4041086\/6241115\/med_012_04_040802.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T22:29:07Z","timestamp":1570400947000},"score":1,"resource":{"primary":{"URL":"https:\/\/asmedigitalcollection.asme.org\/medicaldevices\/article\/doi\/10.1115\/1.4041086\/368713\/Advances-in-Diagnostic-Methods-for-Zika-Virus"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,5]]},"references-count":98,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,12,1]]}},"URL":"https:\/\/doi.org\/10.1115\/1.4041086","relation":{},"ISSN":["1932-6181","1932-619X"],"issn-type":[{"value":"1932-6181","type":"print"},{"value":"1932-619X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,5]]},"article-number":"040802"}}