{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:18:38Z","timestamp":1785403118685,"version":"3.56.0"},"reference-count":38,"publisher":"ASME International","issue":"6","license":[{"start":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T00:00:00Z","timestamp":1710892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.asme.org\/publications-submissions\/publishing-information\/legal-policies"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR071718, T32AR007132, P30AR069619"],"award-info":[{"award-number":["R01AR071718, T32AR007132, P30AR069619"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asmedigitalcollection.asme.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>During pregnancy and breastfeeding, women undergo hormonal fluctuations required for fetal development, parturition, and infant growth. These changes have secondary consequences on the maternal musculoskeletal system, increasing the risk for joint pain and osteoporosis. Though hormone levels return to prepregnancy levels postpartum, women may experience lasting musculoskeletal pain. Sex disparities exist in the prevalence of musculoskeletal disorders, but it remains unclear how reproductive history may impact sex differences. Specifically, the effects of both reproductive history and sex on the rotator cuff have not been studied. Pregnancy and lactation affect bone microstructure, suggesting possible impairments at the enthesis of rotator cuff tendons, where tears commonly occur. Therefore, our objective was to evaluate how reproductive history affects sex differences of the supraspinatus tendon and proximal humerus using male, virgin female, and female rats with a history of reproduction (referred to as reproductive females). We hypothesized tendon mechanical properties and humeral bone microstructure would be inferior in reproductive females compared to virgin females. Results showed sex differences independent of reproductive history, including greater tendon midsubstance modulus but lower subchondral bone mineral density (BMD) in females. When considering reproductive history, reproductive rats exhibited reduced tendon insertion site modulus and trabecular bone micro-architecture compared to virgin females with no differences from males. Overall, our study identified long-term changes in supraspinatus tendon mechanical and humeral trabecular bone properties that result following pregnancy and lactation, highlighting the importance of considering reproductive history in investigations of sex differences in the physiology and pathology of rotator cuff injuries.<\/jats:p>","DOI":"10.1115\/1.4063628","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T12:13:15Z","timestamp":1696421595000},"update-policy":"https:\/\/doi.org\/10.1115\/crossmarkpolicy-asme","source":"Crossref","is-referenced-by-count":3,"title":["Long-Term Effects of Reproduction and Lactation on the Rat Supraspinatus Tendon and Proximal Humerus"],"prefix":"10.1115","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0287-6287","authenticated-orcid":false,"given":"Ashley K.","family":"Fung","sequence":"first","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"name":"Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihan","family":"Li","sequence":"additional","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"name":"Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"id":[{"id":"https:\/\/ror.org\/00b30xv10","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Wang","sequence":"additional","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"name":"Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"id":[{"id":"https:\/\/ror.org\/00b30xv10","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Leahy","sequence":"additional","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"name":"Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104 ; Philadelphia, PA 19104"},{"id":[{"id":"https:\/\/ror.org\/00b30xv10","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snehal S.","family":"Shetye","sequence":"additional","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. Sherry","family":"Liu","sequence":"additional","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, 332A Stemmler Hall, Philadelphia, PA 19104-6081 ; , 3450 Hamilton Walk, Philadelphia, PA 19104-6081"},{"name":"Department of Bioengineering, University of Pennsylvania 332A Stemmler Hall, Philadelphia, PA 19104-6081 ; , 3450 Hamilton Walk, Philadelphia, PA 19104-6081"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis J.","family":"Soslowsky","sequence":"additional","affiliation":[{"name":"McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, 307A Stemmler Hall, Philadelphia, PA 19104-6081 ; , 3450 Hamilton Walk, Philadelphia, PA 19104-6081"},{"name":"Department of Bioengineering, University of Pennsylvania 307A Stemmler Hall, Philadelphia, PA 19104-6081 ; , 3450 Hamilton Walk, Philadelphia, PA 19104-6081"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"33","published-online":{"date-parts":[[2024,3,20]]},"reference":[{"issue":"1","key":"2024032016341291700_bib1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.12891\/ceog1809.2015","article-title":"The Prevalence of Primary Neck and Shoulder Pain, and Its Related Factors in Japanese Postpartum Women","volume":"42","year":"2015","journal-title":"Clin. 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