{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T17:47:17Z","timestamp":1758044837433,"version":"3.44.0"},"reference-count":35,"publisher":"ASME International","issue":"11","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.asme.org\/publications-submissions\/publishing-information\/legal-policies"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR071431"],"award-info":[{"award-number":["R01AR071431"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR071431-03S1"],"award-info":[{"award-number":["R01AR071431-03S1"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-1842473"],"award-info":[{"award-number":["DGE-1842473"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asmedigitalcollection.asme.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study aimed to integrate deeplabcut (dlc) with our Automatic Gait Analysis Through Hues and Areas (AGATHA) algorithm. Prior work with AGATHA shows that it can be used to understand spatiotemporal gait adaptations in multiple disease models. However, AGATHA cannot detect kinematic variables, like joint angles, which dlc was designed to measure. Here, these two approaches are integrated, and the gait variables that can be achieved with both methods are compared. To train dlc, hand digitization of high-speed videos was conducted to estimate the location of several key anatomical markers; then a neural network within dlc was used to automate the digitization of these same points in subsequent videos. A matlab pipeline was developed to calculate average stride profiles for toe height, back angle, and midfoot angle from dlc coordinates. Then, 418 videos of na\u00efve Sprague-Dawley rats (12 w.o., n\u2009=\u200918) walking unprompted across an arena were collected. These videos were analyzed using dlc and AGATHA. For velocity, hind limb duty factor, stride length, and step width, variability was larger in dlc than in AGATHA. However, when used in conjunction, dlc and AGATHA have strong complementary datasets, where AGATHA can provide spatiotemporal and dynamic measures, and dlc can provide kinematic measures that AGATHA cannot currently measure. Decisions on whether to use AGATHA alone, dlc alone, or AGATHA\u2013dlc in tandem are thus dependent on the gait variables being evaluated.<\/jats:p>","DOI":"10.1115\/1.4069390","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T15:26:11Z","timestamp":1755098771000},"update-policy":"https:\/\/doi.org\/10.1115\/crossmarkpolicy-asme","source":"Crossref","is-referenced-by-count":0,"title":["Pipeline Validation for Rodent Gait Analysis Using <scp>deeplabcut<\/scp>\n                  <scp\/>"],"prefix":"10.1115","volume":"147","author":[{"given":"L.","family":"Savannah Dewberry","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, School of Medicine, Duke University , Durham, NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7424-9867","authenticated-orcid":false,"given":"Carlos J.","family":"Cruz","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida , Gainesville, FL 32611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4926-9998","authenticated-orcid":false,"given":"Kaitlin M.","family":"Southern","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida , Gainesville, FL 32611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin J.","family":"Otto","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering, College of Engineering, Purdue University , West Lafayette, IN 47907"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle D.","family":"Allen","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida , Gainesville, FL 32611 ; , Gainesville, FL 32611"},{"name":"Department of Orthopedic Surgery and Sports Medicine, College of Medicine, Pain Research and Intervention Center of Excellence, University of Florida , Gainesville, FL 32611 ; , Gainesville, FL 32611"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"33","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"issue":"3","key":"2025091211294023200_bib1","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.gaitpost.2010.12.010","article-title":"Foot and Ankle Joint Kinematics in Rheumatoid Arthritis Cannot Only Be Explained by Alteration in Walking Speed","volume":"33","year":"2011","journal-title":"Gait Posture"},{"issue":"3","key":"2025091211294023200_bib2","doi-asserted-by":"crossref","first-page":"bjgpopen19X101654","DOI":"10.3399\/bjgpopen19X101654","article-title":"Understanding Sciatica: Illness and Treatment Beliefs in a Lumbar Radicular Pain Population. 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