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The device's stiffness was first validated under dynamic loading, followed by a quantitative analysis of tracer concentrations in cartilage using force-flow coupling. Hydrogel scaffolds were then evaluated for their mass transfer performance, with different peak stresses applied to investigate molecular transport mechanisms within cartilage. Experimental results showed that the stress\u2013time curve of the device was consistent with that of the original fixture. Under the same peak stress, tracer concentration during peristaltic pump-driven bone-to-cartilage fluid transport was 1.40 times higher than that without flow. Mass transfer experiments with cartilage-hydrogel constructs further demonstrated that hydrogel scaffolds exhibited higher molar flow rates when the pump was activated compared to the static state, showing superior mass transfer performance relative to native cartilage. Peak stress regulation experiments revealed that dynamic loading enhanced tracer transport into the deep cartilage layer under constant flow conditions. The newly developed device facilitates experimental investigations of both native and artificial cartilage, elucidating the complex interactions between mechanical loading and osteochondral interface fluid dynamics. This technology provides crucial technical support for understanding cartilage repair mechanisms, optimizing drug delivery strategies, and evaluating the mass transfer performance of artificial cartilage under physiological loading conditions.<\/jats:p>","DOI":"10.1115\/1.4069534","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T15:40:42Z","timestamp":1756222842000},"update-policy":"https:\/\/doi.org\/10.1115\/crossmarkpolicy-asme","source":"Crossref","is-referenced-by-count":2,"title":["Development and Application of a Novel Dynamic Loading Device for Molecular Transport in Articular Cartilage"],"prefix":"10.1115","volume":"147","author":[{"given":"Yabo","family":"Wang","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"name":"National Demonstration Center for Experimental Mechanical and Electrical Engineering Education, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanliuxing","family":"Yan","sequence":"additional","affiliation":[{"name":"Konee Co., Ltd , Shenzhen 518118, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weichao","family":"Dai","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Bone Implant Interface Functionalization and Personality Research, Just Medical Equipment (Tianjin) Co., Ltd , Tianjin 300190, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilan","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"name":"National Demonstration Center for Experimental Mechanical and Electrical Engineering Education, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"id":[{"id":"https:\/\/ror.org\/00zbe0w13","id-type":"ROR","asserted-by":"publisher"}],"name":"Tianjin University of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xujie","family":"Meng","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"name":"National Demonstration Center for Experimental Mechanical and Electrical Engineering Education, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"id":[{"id":"https:\/\/ror.org\/00zbe0w13","id-type":"ROR","asserted-by":"publisher"}],"name":"Tianjin University of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianglong","family":"Lin","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control, School of Mechanical Engineering, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"name":"National Demonstration Center for Experimental Mechanical and Electrical Engineering Education, Tianjin University of Technology , Tianjin 300384, China ; , Tianjin 300384, China"},{"id":[{"id":"https:\/\/ror.org\/00zbe0w13","id-type":"ROR","asserted-by":"publisher"}],"name":"Tianjin University of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"33","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"issue":"7","key":"2025092707102680700_bib1","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1002\/art.42089","article-title":"Prevalence Trends of Site-Specific Osteoarthritis From 1990 to 2019: Findings From the Global Burden of Disease Study 2019","volume":"74","year":"2022","journal-title":"Arthritis Rheumatol."},{"issue":"1","key":"2025092707102680700_bib2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nrrheum.2013.159","article-title":"Progress in Intra-Articular Therapy","volume":"10","year":"2014","journal-title":"Nat. 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