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However, its hydrophobic nature and limited biocompatibility pose challenges for cardiovascular applications, requiring optimal cell-material interactions. This study investigates the surface modification of silk fibroin-derived peptide\/PCL coatings to enhance biocompatibility and cellular response for potential artificial heart applications. PCL substrates were blended with varying concentrations of silk fibroin-derived peptide (0.2%, 0.5%, and 1% w\/v). Surface characterization was performed using water contact angle measurements, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy. Biocompatibility was evaluated through cell viability assays and CD31 expression analysis. Silk fibroin-derived peptide incorporation significantly improved surface hydrophilicity, with contact angles decreasing from 78\u00b0 (bare PCL) to 62\u00b0 (1% silk fibroin-derived peptide). FTIR analysis confirmed successful incorporation of silk fibroin-derived peptides, showing characteristic PCL ester C=O absorption at \u223c1720\u2009cm\u22121 and concentration-dependent amide I contributions at \u223c1630\u20131670\u2009cm\u22121. Cell viability studies demonstrated enhanced cellular response with increasing silk fibroin-derived peptide concentration, particularly evident at day 7. CD31 expression analysis revealed improved endothelial marker expression, with the highest levels observed on 1% silk fibroin-derived peptide-incorporated substrates. The results demonstrate that silk fibroin blended to PCL significantly enhances biocompatibility through improved hydrophilicity and cellular interactions. Taken together, these preliminary results indicate that silk fibroin-derived peptide-incorporated PCL could represent a promising biomaterial platform for artificial heart applications, with the potential to improve endothelial compatibility and support endothelialization.<\/jats:p>","DOI":"10.1116\/6.0005463","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T11:56:37Z","timestamp":1786017397000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced biocompatibility and endothelialization of silk fibroin-derived peptide-based coating for cardiovascular tissue engineering applications"],"prefix":"10.1116","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2735-7923","authenticated-orcid":false,"given":"Juo","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Convergent Biosystems Engineering, College of Life Sciences, Sunchon National University 1 , 255, Jungang-ro, Suncheon-si, Jeollanam-do 57922,","place":["Republic of Korea"]},{"name":"Interdisciplinary Program in IT-Bio Convergence System, Sunchon National University 2 , 255, Jungang-ro, Suncheon-si, Jeollanam-do 57922,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1543-1041","authenticated-orcid":false,"given":"Jae Woon","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Convergent Biosystems Engineering, College of Life Sciences, Sunchon National University 1 , 255, Jungang-ro, Suncheon-si, Jeollanam-do 57922,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7011-1420","authenticated-orcid":false,"given":"Hoon","family":"Seonwoo","sequence":"additional","affiliation":[{"name":"Department of Convergent Biosystems Engineering, College of Life Sciences, Sunchon National University 1 , 255, Jungang-ro, Suncheon-si, Jeollanam-do 57922,","place":["Republic of Korea"]},{"name":"Interdisciplinary Program in IT-Bio Convergence System, Sunchon National University 2 , 255, Jungang-ro, Suncheon-si, Jeollanam-do 57922,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8761-4897","authenticated-orcid":false,"given":"Hae Yong","family":"Kweon","sequence":"additional","affiliation":[{"name":"Division of Industrial Insect and Sericulture, National Institute of Agricultural Sciences, Rural Development Administration 3 , Wanju-gun, Jeollabuk-do 55365,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-2103","authenticated-orcid":false,"given":"You-Young","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biology, Apiculture Division, National Institute of Agricultural Sciences, Rural Development Administration 4 , Wanju-gun, Jeollabuk-do 55365,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5553-2549","authenticated-orcid":false,"given":"Jeong-Min","family":"Oh","sequence":"additional","affiliation":[{"name":"Clinical Research Institute, Daejeon St. Mary's hospital, College of Medicine, The Catholic University of Korea 5 , 64, Daeheung-ro, Jung-gu, Daejeon 34943,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2219-1835","authenticated-orcid":false,"given":"Dae-Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Daejeon St. Mary's hospital, College of Medicine, The Catholic University of Korea 6 , 64, Daeheung-ro, Jung-gu, Daejeon 34943,","place":["Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"20","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"2026080607563442300_c1","doi-asserted-by":"publisher","first-page":"2982","DOI":"10.1016\/j.jacc.2020.11.010","volume":"76","year":"2020","journal-title":"J. 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