{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:10:55Z","timestamp":1753881055435,"version":"3.41.2"},"reference-count":42,"publisher":"American Vacuum Society","issue":"1","funder":[{"DOI":"10.13039\/501100001871","name":"Ministry of Education and Science | Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/82556\/2011"],"award-info":[{"award-number":["SFRH\/BD\/82556\/2011"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"EC | European Regional Development Fund","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000019","NORTE-01-0145-FEDER-000024"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000019","NORTE-01-0145-FEDER-000024"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pubs.aip.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,2,1]]},"abstract":"<jats:p>The use of targeted nanoparticles for magnetic hyperthermia (MHT) increases MHT selectivity, but often at the expense of its effectiveness. Consequently, targeted MHT is typically used in combination with other treatment modalities. This work describes an implementation of a highly effective monotherapeutic in vitro MHT treatment based on two populations of magnetic particles. Cells were sequentially incubated with two populations of magnetic particles: nonfunctionalized superparamagnetic nanoparticles and anti-CXCR4-functionalized particles. After removing the excess of free particles, an alternating magnetic field (AMF) was applied to produce MHT. The induced cytotoxicity was assessed at different time-points after AMF application. Complete loss of cell viability was observed 72\u2009h after MHT when the iron loading of the anti-CXCR4-functionalized particles was boosted by that of a nontargeted population. Additionally, induction of necrosis resulted in more efficient cell death than did induction of apoptosis. Achieving a uniquely high effectiveness in monotherapeutic MHT demonstrates the potential of this approach to achieve complete loss of viability of cancer cells while avoiding the side effects of dual-treatment strategies that use MHT only as a sensitizing therapy.<\/jats:p>","DOI":"10.1116\/1.5009989","type":"journal-article","created":{"date-parts":[[2018,2,5]],"date-time":"2018-02-05T15:04:16Z","timestamp":1517843056000},"update-policy":"https:\/\/doi.org\/10.1063\/aip-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Combining CXCR4-targeted and nontargeted nanoparticles for effective unassisted <i>in vitro<\/i> magnetic hyperthermia"],"prefix":"10.1116","volume":"13","author":[{"given":"V\u00e2nia","family":"Vilas-Boas","sequence":"first","affiliation":[{"name":"UCIBIO-REQUIMTE, Laboratory of Toxicology, Biological Sciences Department, Faculty of Pharmacy, University of Porto , Rua de Jorge Viterbo Ferreira, 228, 4050\u2013313 Porto, Portugal and , Av. 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Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]},{"given":"Jos\u00e9 A.","family":"Duarte","sequence":"additional","affiliation":[{"name":"CIAFEL, Faculty of Sports, University of Porto , Rua Dr. Pl\u00e1cido da Costa 91, 4200-450 Porto, Portugal"}]},{"given":"Ver\u00f3nica C.","family":"Martins","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory , Av. Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]},{"given":"Dmitri Y.","family":"Petrovykh","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory , Av. Mestre Jos\u00e9 Veiga, 4715-330 Braga, Portugal"}]},{"given":"Paulo P.","family":"Freitas","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory , Av. 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