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Instead of developing a method to classify all chromosome classes, we develop an automatic scheme to detect abnormal metaphase cells by identifying a specific class of chromosomes (class 22) and prescreen for suspicious chronic myeloid leukemia (CML). The scheme includes three steps: (1) iteratively segment randomly distributed individual chromosomes, (2) process segmented chromosomes and compute image features to identify the candidates, and (3) apply an adaptive matching template to identify chromosomes of class 22. An image data set of 451 metaphase cells extracted from bone marrow specimens of 30 positive and 30 negative cases for CML is selected to test the scheme's performance. The overall case-based classification accuracy is 93.3% (100% sensitivity and 86.7% specificity). The results demonstrate the feasibility of applying an automated scheme to detect or prescreen the suspicious cancer cases.<\/jats:p>","DOI":"10.1117\/1.3476336","type":"journal-article","created":{"date-parts":[[2010,8,6]],"date-time":"2010-08-06T18:08:40Z","timestamp":1281118120000},"page":"1-12","source":"Crossref","is-referenced-by-count":13,"title":["Automated identification of abnormal metaphase chromosome cells for the detection of chronic myeloid leukemia using microscopic images"],"prefix":"10.1117","volume":"15","author":[{"given":"Xingwei","family":"Wang","sequence":"first","affiliation":[{"name":"Univ. of Pittsburgh Medical Ctr."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Univ. of Pittsburgh Medical Ctr."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shibo","family":"Li","sequence":"additional","affiliation":[{"name":"Oklahoma Medical Research Foundation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Mulvihill","sequence":"additional","affiliation":[{"name":"The Univ. of Oklahoma Health Sciences Ctr."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Chen","sequence":"additional","affiliation":[{"name":"The Univ. of Oklahoma Bioengineering Ctr."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"The Univ. of Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","reference":[{"key":"10.1117\/1.3476336_r1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-5223.1956.tb03010.x"},{"key":"10.1117\/1.3476336_r2","doi-asserted-by":"publisher","DOI":"10.1088\/0022-3727\/38\/15\/003"},{"key":"10.1117\/1.3476336_r3","first-page":"1497","volume":"142","author":"Nowell","year":"1960","journal-title":"Science","ISSN":"https:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"key":"10.1117\/1.3476336_r4","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.5-5-405"},{"key":"10.1117\/1.3476336_r5","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990010210"},{"key":"10.1117\/1.3476336_r6","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/38\/7\/006"},{"key":"10.1117\/1.3476336_r7","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990160104"},{"key":"10.1117\/1.3476336_r8","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3233\/THC-1996-3403","volume":"3","author":"Beksac","year":"1996","journal-title":"Technol. 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