{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:02:56Z","timestamp":1790816576060,"version":"4.1.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"01","license":[{"start":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T00:00:00Z","timestamp":1390780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2014,1,27]]},"abstract":"<jats:p>Purpose: For generating preclinical pharmacokinetics (PKs) of compounds, blood is drawn at different time points and levels are quantified by different analytical methods. In order to receive statistically meaningful data, 3 to 5 animals are used for each time point to get serum peak-level and half-life of the compound. Both characteristics are determined by data interpolation, which may influence the accuracy of these values. We provide a method that allows continuous monitoring of blood levels noninvasively by measuring the fluorescence intensity of labeled compounds in the eye and other body regions of anesthetized mice. Procedures: The method evaluation was performed with four different fluorescent compounds: (i) indocyanine green, a nontargeting dye; (ii) OsteoSense750, a bone targeting agent; (iii) tumor targeting Trastuzumab-Alexa750; and (iv) its F(ab \u2032 ) 2  -alxea750 fragment. The latter was used for a direct comparison between fluorescence imaging and classical blood analysis using enzyme-linked immunosorbent assay (ELISA). Results: We found an excellent correlation between blood levels measured by noninvasive eye imaging with the results generated by classical methods. A strong correlation between eye imaging and ELISA was demonstrated for the F(ab \u2032 ) 2   fragment. Whole body imaging revealed a compound accumulation in the expected regions (e.g., liver, bone). Conclusions: The combination of eye and whole body fluorescence imaging enables the simultaneous measurement of blood PKs and biodistribution of fluorescent-labeled compounds.<\/jats:p>","DOI":"10.1117\/1.jbo.19.1.016022","type":"journal-article","created":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T10:17:38Z","timestamp":1390904258000},"page":"1-9","source":"Crossref","is-referenced-by-count":10,"title":["Noninvasive measurement of pharmacokinetics by near-infrared fluorescence imaging in the eye of mice"],"prefix":"10.1117","volume":"19","author":[{"given":"Michael","family":"Dobosz","sequence":"first","affiliation":[{"name":"Roche Diagnostics GmbH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Strobel","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay-Gunnar","family":"Stubenrauch","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Osl","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Scheuer","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-19\/issue-01\/016022\/Noninvasive-measurement-of-pharmacokinetics-by-near-infrared-fluorescence-imaging-in\/10.1117\/1.JBO.19.1.016022.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:31:59Z","timestamp":1790814719000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-19\/issue-01\/016022\/Noninvasive-measurement-of-pharmacokinetics-by-near-infrared-fluorescence-imaging-in\/10.1117\/1.JBO.19.1.016022.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,27]]},"references-count":0,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2014,1,1]]}},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.19.1.016022","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,27]]}}}