{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:25:23Z","timestamp":1792787123192,"version":"4.5.0"},"reference-count":22,"publisher":"SPIE-Intl Soc Optical Eng","issue":"05","funder":[{"name":"National Research Foundation of Korea funded by the Ministry of Education","award":["2013R1A1A2010028"],"award-info":[{"award-number":["2013R1A1A2010028"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2014,1,13]]},"abstract":"<jats:p>The characteristics of a waveguide-coupled bimetallic surface plasmon resonance (WcBiM SPR) sensor using (3-dimethylaminopropyl)-3-ethylcarbodiimide(EDC)-N-hydroxysuccinimide(NHS)-activated protein A was investigated, and the detection of IgG using the EDC-NHS-activated protein A was studied in comparison with protein A and a self-assembled monolayer (SAM). The WcBiM sensor, which has a narrower full width at half maximum (FWHM) and a steeper slope, was selected since it leads to a larger change in the reflectance in the intensity detection mode. A preparation of the EDC-NHS-activated protein A for site-directed immobilization of antibodies was relative easily compared to the engineered protein G and A. In antigen\u2013antibody interactions, the response to IgG at the concentrations of 50, 100, and 150\u2009\u2009ng\/ml  was investigated. The results showed that the sensitivity of the WcBiM sensor using the EDC-NHS-activated protein A, protein A, and SAM was 0.0185 [%\/(ng\/ml) ], 0.0065 [%\/(ng\/ml) ], and 0.0101 [%\/(ng\/ml) ], respectively. The lowest detectable concentrations of IgG with the EDC-NHS-activated protein A, protein A, and SAM were 4.27, 12.83, and 8.24\u2009\u2009ng\/ml , respectively. Therefore, the increased sensitivity and lower detection capability of the WcBiM SPR chip with the EDC-NHS-activated protein A suggests that it could be used in early diagnosis where the trace level concentrations of biomolecules should be detected.<\/jats:p>","DOI":"10.1117\/1.jbo.19.5.051209","type":"journal-article","created":{"date-parts":[[2014,1,13]],"date-time":"2014-01-13T11:30:53Z","timestamp":1389612653000},"page":"1-7","source":"Crossref","is-referenced-by-count":33,"title":["Site-directed immobilization of antibody using EDC-NHS-activated protein A on a bimetallic-based surface plasmon resonance chip"],"prefix":"10.1117","volume":"19","author":[{"given":"Young-Soo","family":"Sohn","sequence":"first","affiliation":[{"name":"Catholic Univ. of Daegu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon Kyung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.2147\/NSA.S13465"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.59.1.1"},{"key":"r3","author":"Turner","year":"1987"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2011.02.001"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2012.05.018"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.3938\/jkps.62.475"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.03.011"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1166\/jno.2011.1179"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.06.034"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4005(98)00321-9"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1080\/02726340801921650"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1007\/s13320-011-0051-2"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1016\/j.mee.2013.02.088"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1002\/(ISSN)1097-0290"},{"key":"r15","first-page":"1695","article-title":"Complete sequence of the staphylococcal gene encoding protein A","author":"Uhlen","year":"1984"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1111\/ejb.1977.78.issue-2"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2009.01.026"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics2030023"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1021\/ac0619231"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1039\/c3an36498d"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1186\/1556-276X-8-344"},{"key":"r22","first-page":"45","article-title":"Surface plasmon resonance (SPR) sensors","author":"Homola","year":"2006"}],"container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-19\/issue-05\/051209\/Site-directed-immobilization-of-antibody-using-EDC-NHS-activated-protein\/10.1117\/1.JBO.19.5.051209.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:38:52Z","timestamp":1790620732000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-19\/issue-05\/051209\/Site-directed-immobilization-of-antibody-using-EDC-NHS-activated-protein\/10.1117\/1.JBO.19.5.051209.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,13]]},"references-count":22,"journal-issue":{"issue":"05"},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.19.5.051209","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,13]]}}}