{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T05:19:27Z","timestamp":1793164767307,"version":"4.5.0"},"reference-count":63,"publisher":"SPIE-Intl Soc Optical Eng","issue":"04","license":[{"start":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T00:00:00Z","timestamp":1556064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["EY007125"],"award-info":[{"award-number":["EY007125"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NIH Training","award":["EY007125"],"award-info":[{"award-number":["EY007125"]}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY007125"],"award-info":[{"award-number":["EY007125"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IIP-1346453"],"award-info":[{"award-number":["IIP-1346453"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IIP-1534701"],"award-info":[{"award-number":["IIP-1534701"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IIP-1534701"],"award-info":[{"award-number":["IIP-1534701"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2019,4,24]]},"abstract":"<jats:p>To identify the microstructural modification of the corneal layers during the course of the disease, optical technologies have been pushing the boundary of innovation to achieve cellular resolution of deep layers of the cornea. Gabor-domain optical coherence microscopy (GD-OCM), an optical coherence tomography-based technique that can achieve an isotropic of \u223c2-\u03bcm resolution over a volume of 1\u2009\u2009mm\u2009\u2009\u00d7\u2009\u20091\u2009\u2009mm\u2009\u2009\u00d7\u2009\u20091.2\u2009\u2009mm, was developed to investigate the microstructural modifications of corneal layers in four common corneal diseases. Since individual layer visualization without cutting through several layers is challenging due to corneal curvature, a flattening algorithm was developed to remove the global curvature of the endothelial layer and display the full view of the endothelium and Descemet\u2019s membrane in single en face images. As a result, GD-OCM revealed the qualitative changes in size and reflectivity of keratocytes in Fuchs endothelial corneal dystrophy (FECD), which varied by the degree of disease. More importantly, elongated shape and hyperactivation characteristics of keratocytes, associated with the early development of guttae, appeared to start in the posterior stroma very early in the disease process and move toward the anterior stroma during disease progression. This work opens a venue into the pathogenesis of FECD.<\/jats:p>","DOI":"10.1117\/1.jbo.24.4.046002","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T04:43:38Z","timestamp":1556081018000},"page":"1-17","source":"Crossref","is-referenced-by-count":6,"title":["Capabilities of Gabor-domain optical coherence microscopy for the assessment of corneal disease"],"prefix":"10.1117","volume":"24","author":[{"given":"Patrice","family":"Tankam","sequence":"first","affiliation":[{"name":"Indiana Univ."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"He","sequence":"additional","affiliation":[{"name":"Biologie, Ing\u00e9nierie et Imagerie de la Greffe de Corn\u00e9e, Univ. Jean Monnet Saint-Etienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Thuret","sequence":"additional","affiliation":[{"name":"Biologie, Ing\u00e9nierie et Imagerie de la Greffe de Corn\u00e9e, Univ. Jean Monnet Saint-Etienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly B.","family":"Hindman","sequence":"additional","affiliation":[{"name":"The Eye Care Ctr."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4057-4987","authenticated-orcid":false,"given":"Cristina","family":"Canavesi","sequence":"additional","affiliation":[{"name":"LighTopTech Corp."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johana","family":"Coyoc Escudero","sequence":"additional","affiliation":[{"name":"The Institute of Optics, Univ. of Rochester"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-920X","authenticated-orcid":false,"given":"Thierry","family":"L\u00e9pine","sequence":"additional","affiliation":[{"name":"Lab. Hubert Curien, Univ. de Lyon, CNRS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Gain","sequence":"additional","affiliation":[{"name":"Biologie, Ing\u00e9nierie et Imagerie de la Greffe de Corn\u00e9e, Univ. Jean Monnet Saint-Etienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2747-4022","authenticated-orcid":false,"given":"Jannick P.","family":"Rolland","sequence":"additional","affiliation":[{"name":"LighTopTech Corp."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2015.4776"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(76)90247-6"},{"key":"r3","first-page":"597","article-title":"Regenerative capacity of the corneal endothelium in rabbit and cat","author":"Van Horn","year":"1977"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.83.2.185"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2005.12.022"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2009.06.022"},{"key":"r7","first-page":"435","article-title":"Clinical applications of corneal confocal microscopy","author":"Tavakoli","year":"2008"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2008.137430"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2009.11.001"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200003000-00006"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31815892da"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000000698"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1126\/science.1957169"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1117\/12.231361"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.7.071412"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2018.04.002"},{"key":"r17","doi-asserted-by":"publisher","DOI":"10.1038\/86589"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1364\/OL.26.000608"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1364\/OL.27.001800"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.003037"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0584"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1117\/1.2764461"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2014.935444"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1111\/aos.2017.95.issue-4"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.000557"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32814-3"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1364\/OPTICA.5.000409"},{"key":"r28","doi-asserted-by":"publisher","DOI":"10.1364\/OL.19.000590"},{"key":"r29","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.8.000800"},{"key":"r30","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.006569"},{"key":"r31","doi-asserted-by":"publisher","DOI":"10.1364\/OL.28.002064"},{"key":"r32","doi-asserted-by":"publisher","DOI":"10.1117\/12.816930"},{"key":"r33","doi-asserted-by":"publisher","DOI":"10.1364\/OL.34.000145"},{"key":"r34","doi-asserted-by":"publisher","DOI":"10.1364\/OE.18.003632"},{"key":"r35","doi-asserted-by":"publisher","DOI":"10.1364\/OL.41.000508"},{"key":"r36","doi-asserted-by":"publisher","DOI":"10.1364\/OL.40.001113"},{"key":"r37","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.8.002906"},{"key":"r38","doi-asserted-by":"publisher","DOI":"10.1364\/AO.44.004009"},{"key":"r39","doi-asserted-by":"publisher","DOI":"10.1364\/OE.18.023378"},{"key":"r40","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.7.071410"},{"key":"r41","year":"2007"},{"key":"r42","first-page":"36","article-title":"Image processing with ImageJ","author":"Abramoff","year":"2004"},{"key":"r43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2002.282.1.C213"},{"key":"r44","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.001524"},{"key":"r45","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.5.056003"},{"key":"r46","first-page":"171","article-title":"An automatic algorithm for segmentation of the boundaries of corneal layers in optical coherence tomography images using gaussian mixture model","author":"Jahromi","year":"2014"},{"key":"r47","article-title":"Peatdet: peak detection using MATLAB","author":"Billauer","year":"2012"},{"key":"r48","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1978.03910060424004"},{"key":"r49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2015.05.002"},{"key":"r50","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.22.1.010501"},{"key":"r51","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2010.12.037"},{"key":"r52","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(81)34959-8"},{"key":"r53","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000148288.53323.b2"},{"key":"r54","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13194-6"},{"key":"r55","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70232-X"},{"key":"r56","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S83467"},{"key":"r57","doi-asserted-by":"publisher","DOI":"10.1046\/j.1475-1313.1999.00408.x"},{"key":"r58","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0497"},{"key":"r59","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.10.023"},{"key":"r60","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2010.344"},{"key":"r61","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2013.799"},{"key":"r62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2012.09.022"},{"key":"r63","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-18856"}],"container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-24\/issue-04\/046002\/Capabilities-of-Gabor-domain-optical-coherence-microscopy-for-the-assessment\/10.1117\/1.JBO.24.4.046002.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:03:30Z","timestamp":1790294610000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-24\/issue-04\/046002\/Capabilities-of-Gabor-domain-optical-coherence-microscopy-for-the-assessment\/10.1117\/1.JBO.24.4.046002.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,24]]},"references-count":63,"journal-issue":{"issue":"04"},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.24.4.046002","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,24]]}}}