{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:02:40Z","timestamp":1790294560695,"version":"4.1.0"},"reference-count":27,"publisher":"SPIE-Intl Soc Optical Eng","issue":"08","license":[{"start":{"date-parts":[[2019,8,24]],"date-time":"2019-08-24T00:00:00Z","timestamp":1566604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSF CBET","award":["1402345"],"award-info":[{"award-number":["1402345"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2019,8,24]]},"abstract":"<jats:p>&lt;p&gt;Angularly resolved elastic light scattering is an established technique for probing the average size of organelles in biological tissue and cellular ensembles. Focusing of the incident light to illuminate no more than one cell at a time restricts the minimum forward-scattering angle \u03b8&lt;sub&gt;min&lt;\/sub&gt; that can be detected. Series of simulated single-cell angular-scattering patterns have been generated to explore how size estimates vary as a function of \u03b8&lt;sub&gt;min&lt;\/sub&gt;. At a setting of \u03b8&lt;sub&gt;min&lt;\/sub&gt;\u2009\u2009=\u2009\u200920\u2009\u2009deg, the size estimates hop unstably between multiple minima in the solution space as simulated noise (mimicking experimentally observed levels) is varied. As \u03b8&lt;sub&gt;min&lt;\/sub&gt; is reduced from 20\u00a0deg to 10\u00a0deg, the instability vanishes, and the variance of estimates near the correct answer also decreases. The simulations thus suggest that robust Mie theory fits to single-cell scattering at 785\u00a0nm excitation require measurements down to at least 15\u00a0deg. Notably, no such instability was observed at \u03b8&lt;sub&gt;min&lt;\/sub&gt;\u2009\u2009=\u2009\u200920\u2009\u2009deg for narrow bead distributions. Accurate sizing of traditional calibration beads is, therefore, insufficient proof that an angular-scattering system is capable of robust analysis of single cells. Experimental support for the simulation results is also presented using measurements on cells fixed with formaldehyde.&lt;\/p&gt;<\/jats:p>","DOI":"10.1117\/1.jbo.24.8.086502","type":"journal-article","created":{"date-parts":[[2019,8,24]],"date-time":"2019-08-24T02:32:37Z","timestamp":1566613957000},"page":"1-12","source":"Crossref","is-referenced-by-count":5,"title":["Angularly resolved, finely sampled elastic scattering measurements of single cells: requirements for robust organelle size extractions"],"prefix":"10.1117","volume":"24","author":[{"given":"Ashley E.","family":"Cannaday","sequence":"first","affiliation":[{"name":"The Institute of Optics, Univ. of Rochester"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0071-8509","authenticated-orcid":false,"given":"Janet","family":"Sorrells","sequence":"additional","affiliation":[{"name":"Univ. of Rochester"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5090-1198","authenticated-orcid":false,"given":"Andrew J.","family":"Berger","sequence":"additional","affiliation":[{"name":"The Institute of Optics, Univ. of Rochester"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.80.627"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1109\/2944.796325"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1109\/JSTQE.2003.814183"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.048736"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1002\/(ISSN)1521-3889"},{"key":"r6","author":"Bohren","year":"1983"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1088\/0150-536X\/16\/2\/004"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1088\/0150-536X\/16\/5\/007"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1364\/AO.25.003539"},{"key":"r10","doi-asserted-by":"publisher","DOI":"10.1364\/JOSAA.7.000998"},{"key":"r11","doi-asserted-by":"publisher","DOI":"10.1364\/OL.33.002452"},{"key":"r12","doi-asserted-by":"publisher","DOI":"10.1364\/OE.16.005926"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1117\/1.1483317"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.054528"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1117\/1.2437765"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1364\/OL.32.002348"},{"key":"r17","article-title":"Holographic angular domain elastic scattering of single biological cells","author":"Shipp","year":"2014"},{"key":"r18","doi-asserted-by":"publisher","DOI":"10.1364\/AO.37.003586"},{"key":"r19","doi-asserted-by":"publisher","DOI":"10.1117\/1.429979"},{"key":"r20","doi-asserted-by":"publisher","DOI":"10.1364\/OL.30.002442"},{"key":"r21","doi-asserted-by":"publisher","DOI":"10.20982\/tqmp.10.2.p107"},{"key":"r22","doi-asserted-by":"publisher","DOI":"10.1364\/AO.48.00D109"},{"key":"r23","doi-asserted-by":"publisher","DOI":"10.1364\/OE.11.003473"},{"key":"r24","doi-asserted-by":"publisher","DOI":"10.1117\/1.3462933"},{"key":"r25","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.019674"},{"key":"r26","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.101.238102"},{"key":"r27","doi-asserted-by":"publisher","DOI":"10.1364\/OL.33.001596"}],"container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-24\/issue-08\/086502\/Angularly-resolved-finely-sampled-elastic-scattering-measurements-of-single-cells\/10.1117\/1.JBO.24.8.086502.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:48:56Z","timestamp":1790293736000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-24\/issue-08\/086502\/Angularly-resolved-finely-sampled-elastic-scattering-measurements-of-single-cells\/10.1117\/1.JBO.24.8.086502.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,24]]},"references-count":27,"journal-issue":{"issue":"08"},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.24.8.086502","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,24]]}}}