{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:02:35Z","timestamp":1790838155690,"version":"4.1.0"},"reference-count":40,"publisher":"SPIE-Intl Soc Optical Eng","issue":"01","license":[{"start":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T00:00:00Z","timestamp":1580342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2020,1,30]]},"abstract":"<jats:p>&lt;p&gt;Two-photon microscopes have been successfully translated into clinical imaging tools to obtain high-resolution optical biopsies for &lt;italic&gt;in vivo&lt;\/italic&gt; histology. We report on clinical multiphoton coherent anti-Stokes Raman spectroscopy (CARS) tomography based on two tunable ultrashort near-infrared laser beams for label-free &lt;italic&gt;in vivo&lt;\/italic&gt; multimodal skin imaging. The multiphoton biopsies were obtained with the compact tomograph \u201cMPTflex-CARS\u201d using a photonic crystal fiber, an optomechanical articulated arm, and a four-detector-360\u00a0deg measurement head. The multiphoton tomograph has been employed to patients in a hospital with diseased skin. The clinical study involved 16 subjects, 8 patients with atopic dermatitis, 4 patients with psoriasis vulgaris, and 4 volunteers served as control. Two-photon cellular autofluorescence lifetime, second harmonic generation (SHG) of collagen, and CARS of intratissue lipids\/proteins have been detected with single-photon sensitivity, submicron spatial resolution, and picosecond temporal resolution. The most important signal was the autofluorescence from nicotinamide adenine dinucleotide [NAD(P)H]. The SHG signal from collagen was mainly used to detect the epidermal\u2013dermal junction and to calculate the ratio elastin\/collagen. The CARS\/Raman signal provided add-on information. Based on this view on the disease-affected skin on a subcellular level, skin areas affected by dermatitis and by psoriasis could be clearly identified. Multimodal multiphoton tomographs may become important label-free clinical high-resolution imaging tools for &lt;italic&gt;in vivo&lt;\/italic&gt; skin histology to realize rapid early diagnosis as well as treatment control.&lt;\/p&gt;<\/jats:p>","DOI":"10.1117\/1.jbo.25.1.014515","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T11:21:41Z","timestamp":1580383301000},"page":"1-12","source":"Crossref","is-referenced-by-count":46,"title":["Translation of two-photon microscopy to the clinic: multimodal multiphoton CARS tomography of in vivo human skin"],"prefix":"10.1117","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-9705","authenticated-orcid":false,"given":"Karsten","family":"K\u00f6nig","sequence":"first","affiliation":[{"name":"JenLab GmbH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Georg","family":"Breunig","sequence":"additional","affiliation":[{"name":"JenLab 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