{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:00:26Z","timestamp":1790276426332,"version":"4.1.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"03","license":[{"start":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T00:00:00Z","timestamp":1583452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CBET 1751554"],"award-info":[{"award-number":["CBET 1751554"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Cancer Institute","award":["1R15 CA202662"],"award-info":[{"award-number":["1R15 CA202662"]}],"id":[{"id":"https:\/\/ror.org\/040gcmg81","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Arkansas Biosciences Institute","award":["Fulbright Faculty Development Award"],"award-info":[{"award-number":["Fulbright Faculty Development Award"]}],"id":[{"id":"https:\/\/ror.org\/04ptmx344","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2020,3,6]]},"abstract":"<jats:p>Significance: Many studies in colorectal cancer (CRC) use murine ectopic tumor models to determine response to treatment. However, these models do not replicate the tumor microenvironment of CRC. Physiological information of treatment response derived via diffuse reflectance spectroscopy (DRS) from murine primary CRC tumors provide a better understanding for the development of new drugs and dosing strategies in CRC.\nAim: Tumor response to chemotherapy in a primary CRC model was quantified via DRS to extract total hemoglobin content (tHb), oxygen saturation (StO2), oxyhemoglobin, and deoxyhemoglobin in tissue.\nApproach: A multimodal DRS and imaging probe (0.78\u00a0mm outside diameter) was designed and validated to acquire diffuse spectra longitudinally\u2014via endoscopic guidance\u2014in developing colon tumors under 5-fluoruracil (5-FU) maximum-tolerated (MTD) and metronomic regimens. A filtering algorithm was developed to compensate for positional uncertainty in DRS measurements\nResults: A maximum increase in StO2 was observed in both MTD and metronomic chemotherapy-treated murine primary CRC tumors at week 4 of neoadjuvant chemotherapy, with 21\u2009\u2009\u00b1\u2009\u20096\u2009\u2009%\u2009\u2009 and 17\u2009\u2009\u00b1\u2009\u20096\u2009\u2009%\u2009\u2009 fold changes, respectively. No significant changes were observed in tHb.\nConclusion: Our study demonstrates the feasibility of DRS to quantify response to treatment in primary CRC models.<\/jats:p>","DOI":"10.1117\/1.jbo.25.3.035002","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T22:37:18Z","timestamp":1583447838000},"page":"1-16","source":"Crossref","is-referenced-by-count":11,"title":["Diffuse reflectance spectroscopy to monitor murine colorectal tumor progression and therapeutic response"],"prefix":"10.1117","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6014-4538","authenticated-orcid":false,"given":"Ariel I.","family":"Mundo","sequence":"first","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5274-9932","authenticated-orcid":false,"given":"Gage J.","family":"Greening","sequence":"additional","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Fahr","sequence":"additional","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9379-3147","authenticated-orcid":false,"given":"Lawrence N.","family":"Hale","sequence":"additional","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7736-0536","authenticated-orcid":false,"given":"Elizabeth A.","family":"Bullard","sequence":"additional","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1224-8567","authenticated-orcid":false,"given":"Narasimhan","family":"Rajaram","sequence":"additional","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Muldoon","sequence":"additional","affiliation":[{"name":"Univ. of Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-25\/issue-03\/035002\/Diffuse-reflectance-spectroscopy-to-monitor-murine-colorectal-tumor-progression-and\/10.1117\/1.JBO.25.3.035002.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:16:44Z","timestamp":1790273804000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-25\/issue-03\/035002\/Diffuse-reflectance-spectroscopy-to-monitor-murine-colorectal-tumor-progression-and\/10.1117\/1.JBO.25.3.035002.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,6]]},"references-count":0,"journal-issue":{"issue":"03"},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.25.3.035002","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,6]]}}}