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Current standard-of-care diagnostic imaging techniques lack the specificity needed to differentiate these states, leading to false positives.AimWe introduce multiparameter interferometric polarization-enhanced (MultiPIPE) imaging as a promising technology to improve the specificity of detection for better biopsy guidance and clinical outcomes.ApproachIn this ex vivo study, we extract tissue attenuation-coefficient-based and birefringence-based parameters from MultiPIPE imaging data, collected with a bench-top system, to develop a classifier for the differentiation of benign and CIS tissues. We also analyze morphological features from second harmonic generation imaging and histology slides and perform imaging-to-morphology correlation analysis.ResultsMultiPIPE enhances specificity to differentiate CIS from benign tissues by nearly 20% and reduces the false-positive rate by more than four-fold over clinical standards. We also show that the MultiPIPE measurements correlate well with changes in morphological features in histological assessments.ConclusionsThe results of our study show the promise of MultiPIPE imaging to be used for better differentiation of bladder inflammation from flat tumors, leading to a fewer number of unnecessary procedures and shorter operating room (OR) time.<\/jats:p>","DOI":"10.1117\/1.jbo.28.10.102907","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T18:08:31Z","timestamp":1691690911000},"page":"1-18","source":"Crossref","is-referenced-by-count":0,"title":["Multiparameter interferometric polarization-enhanced imaging differentiates carcinoma in situ from inflammation of the bladder: an ex vivo study"],"prefix":"10.1117","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3313-0683","authenticated-orcid":false,"given":"Shuang","family":"Chang","sequence":"first","affiliation":[{"name":"Vannderbilt University, Vanderbilt 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