{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:05:05Z","timestamp":1790208305693,"version":"4.1.0"},"reference-count":40,"publisher":"SPIE-Intl Soc Optical Eng","issue":"12","license":[{"start":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T00:00:00Z","timestamp":1734912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2024,12,23]]},"abstract":"<jats:p>SignificanceAlzheimer\u2019s disease (AD) is a predominant form of dementia that can lead to a decline in the quality of life and mortality. The understanding of the pathological changes requires monitoring of multiple cerebral biomarkers simultaneously with high resolution. Photoacoustic microscopy resolves single capillaries, allowing investigations into the most affected types of vessels. Combined with confocal fluorescence microscopy, the relationship between plaque deposition and small vessel pathology could be better understood.AimWe aim to introduce a dual-modality imaging system combining photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) to provide a comprehensive view of both cerebral cortical vessels and amyloid-\u03b2 (A\u03b2) plaque in AD mouse model in vivo and to identify the pathological changes of these two biomarkers.ApproachWe developed a dual-modality imaging system to image both cerebral vessel structure and A\u03b2 plaque on groups of mice with different ages and phenotypes. Vessel imaging is enabled by PAM, whereas A\u03b2 plaque is imaged by CFM with the aid of fluorescent dye.ResultsThe small vessel density in the AD group was significantly lower than in the control group, whereas the A\u03b2 plaque density in the AD group was not only higher but also increased with age.ConclusionsThis dual-modality system provides a powerful platform for biomarker monitoring of AD expressing multi-dimensional pathological changes.<\/jats:p>","DOI":"10.1117\/1.jbo.29.12.126002","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T08:16:33Z","timestamp":1734941793000},"page":"1-13","source":"Crossref","is-referenced-by-count":5,"title":["Photoacoustic and fluorescence dual-modality imaging of cerebral biomarkers in Alzheimer\u2019s disease rodent model"],"prefix":"10.1117","volume":"29","author":[{"given":"Tianqu","family":"Zhai","sequence":"first","affiliation":[{"name":"University of 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