{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:04:30Z","timestamp":1790197470078,"version":"4.1.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"11","license":[{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["#RGPIN-2015-04672"],"award-info":[{"award-number":["#RGPIN-2015-04672"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000156","name":"Fonds de Recherche du Qu\u00e9bec\u2014Sant\u00e9","doi-asserted-by":"publisher","award":["#32600"],"award-info":[{"award-number":["#32600"]}],"id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2025,11,3]]},"abstract":"<jats:p>SignificanceIn vivo optical coherence tomography (OCT) of the mouse vitreoretinal vasculature in full depth is technically challenging. Conventional OCT techniques employ axial confocal gating, which induces signal drop-off and limits spatial resolution outside the Rayleigh range.AimOur aim is to develop a multifocal OCT imaging approach using a tunable lens and a registration method that allows the generation of a composite image of the vitreoretinal vasculature while preserving high and uniform lateral spatial resolution, signal intensity, and image contrast in full depth.ApproachA calibration target was developed to characterize the multifocal optical system and quantify the signal intensity, contrast, and resolution. These optical specifications were used to image mice at postnatal day 14. Intra- and inter-volume registration methods were necessary to correct for motion and generate a composite image from single-focus images using weighted averaging.ResultsIn the calibration target, signal intensity and contrast were 20\u00a0dB higher in the composite compared with single-focus images. Lateral resolution remained uniform (4 to 6 \u03bcm). In animals, signal intensity and contrast were 10 to 15\u00a0dB higher in the composite compared with single-focus images and highest in the hyaloid vasculature.ConclusionsThis technique is promising in studying the mouse vitreoretinal vasculature during eye development and disease.<\/jats:p>","DOI":"10.1117\/1.jbo.30.11.116002","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T19:07:17Z","timestamp":1762196837000},"page":"1-13","source":"Crossref","is-referenced-by-count":0,"title":["Multifocal optical coherence tomography of the mouse eye to image the vitreoretinal vasculature in full depth"],"prefix":"10.1117","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6519-8423","authenticated-orcid":false,"given":"Simon","family":"Brais-Brunet","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Montr\u00e9al"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00ebl","family":"Maltais-Tariant","sequence":"additional","affiliation":[{"name":"Polytechnique Montr\u00e9al"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2145-9086","authenticated-orcid":false,"given":"Caroline","family":"Boudoux","sequence":"additional","affiliation":[{"name":"CHU Sainte-Justine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9852-6761","authenticated-orcid":false,"given":"Mathieu","family":"Dehaes","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Montr\u00e9al"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-30\/issue-11\/116002\/Multifocal-optical-coherence-tomography-of-the-mouse-eye-to-image\/10.1117\/1.JBO.30.11.116002.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:33:01Z","timestamp":1790195581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-30\/issue-11\/116002\/Multifocal-optical-coherence-tomography-of-the-mouse-eye-to-image\/10.1117\/1.JBO.30.11.116002.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,3]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11,1]]}},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.30.11.116002","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,3]]}}}