{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:25:34Z","timestamp":1792787134543,"version":"4.5.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"03","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICMR-Indian Council of Medical Research","award":["56\/2\/2020-Hae\/BMS"],"award-info":[{"award-number":["56\/2\/2020-Hae\/BMS"]}],"id":[{"id":"https:\/\/ror.org\/0492wrx28","id-type":"ROR","asserted-by":"crossref"}]},{"name":"DBT-Department of Biotechnology","award":["BT\/PR44547\/MED\/32\/791\/2021"],"award-info":[{"award-number":["BT\/PR44547\/MED\/32\/791\/2021"]}],"id":[{"id":"https:\/\/ror.org\/03tjsyq23","id-type":"ROR","asserted-by":"crossref"}]},{"name":"ANRF-Anusandhan National Research Foundation","award":["CRG\/2023\/003278"],"award-info":[{"award-number":["CRG\/2023\/003278"]}],"id":[{"id":"https:\/\/ror.org\/00d4e4338","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2026,3,2]]},"abstract":"<jats:p>SignificanceAnemia is a global health concern, prompting the need for rapid, accurate, and noninvasive diagnostic tools. This has led to significant interest in the development of various optical tools, including photoacoustic (PA) spectroscopy for monitoring and quantification of clinically relevant blood parameters.AimEstimating the blood lysis level (LL) and oxygenation (SO2) is essential for the detection of various hemolytic conditions, including anemia. The PA spectroscopy is explored here for quantifying hemolytic blood samples.ApproachIn vitro PA measurements on human blood samples were validated through simulation studies involving discrete dipole approximation, Monte Carlo, and k-Wave methods. Blood hematocrit (H), LL, and SO2 levels are determined from simulated and experimental PA signals.ResultsThe wavelength pairs 700-905 and 700-1000\u00a0nm are found to be optimal for the estimation of H and SO2 with high accuracy (&gt;90%). The correlation coefficient between the actual and evaluated LLs is calculated to be \u22480.90.ConclusionsResults show that PA measurements with a suitable combination of optical wavelengths can be used for determining the important blood parameters accurately and simultaneously. Further investigation is needed to apply the developed method under an in vivo setting.<\/jats:p>","DOI":"10.1117\/1.jbo.31.3.035001","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T03:19:42Z","timestamp":1772421582000},"page":"1-23","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive study on the characterization of lysed blood samples using dual-wavelength photoacoustics"],"prefix":"10.1117","volume":"31","author":[{"given":"Subhadip","family":"Paul","sequence":"first","affiliation":[{"name":"Indian Institute of Information Technology, Allahabad"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari Shankar","family":"Patel","sequence":"additional","affiliation":[{"name":"Raja Ramanna Centre for Advanced Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vatsala","family":"Misra","sequence":"additional","affiliation":[{"name":"Moti Lal Nehru Medical College"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Rani","sequence":"additional","affiliation":[{"name":"Moti Lal Nehru Medical College"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaresh K.","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Indian Institute of Information Technology, Allahabad"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7274-6707","authenticated-orcid":false,"given":"Ratan K.","family":"Saha","sequence":"additional","affiliation":[{"name":"Indian Institute of Information Technology, Allahabad"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-31\/issue-03\/035001\/Comprehensive-study-on-the-characterization-of-lysed-blood-samples-using\/10.1117\/1.JBO.31.3.035001.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:18:02Z","timestamp":1790194682000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-31\/issue-03\/035001\/Comprehensive-study-on-the-characterization-of-lysed-blood-samples-using\/10.1117\/1.JBO.31.3.035001.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,2]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2026,3,1]]}},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.31.3.035001","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,2]]}}}