{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:02:35Z","timestamp":1790197355598,"version":"4.1.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"03","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R01CA288379"],"award-info":[{"award-number":["R01CA288379"]}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RP240289"],"award-info":[{"award-number":["RP240289"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Opt."],"published-print":{"date-parts":[[2026,3,18]]},"abstract":"<jats:p>SignificanceEarly detection of Alzheimer\u2019s diseases, diabetic retinopathy, or macular degeneration with advanced retinal imaging technologies can help improve patient care and treatment outcome.AimWe aim to create a high-resolution hyperspectral imaging (HSI) system for the retina. Retinal vessel diameter and oxygenation rate will be extracted simultaneously from HSI data.ApproachOur hyperspectral retinal imaging system consists of a snapshot hyperspectral camera, a high-resolution RGB camera, a beamsplitter, and an imaging endoscope. Multiple pansharpening algorithms, including deep learning methods, were developed to generate high-resolution hyperspectral images that were further used for the measurement of vessel size and oxygenation rate in mice.ResultsThe hyperspectral retinal imaging system was tested for its spatial resolution and spectral fidelity in retina phantoms. In vivo imaging experiments were performed in mice. The deep learning-based pansharpening algorithm achieved a root mean square error (RMSE) of 2.15\u00b10.64, a correlation coefficient (CC) of 0.96\u00b10.05, a spectral angle score of 0.06\u00b10.03 radians, and an error relative global dimensionless synthesis (ERGAS) score of 2.37\u00b11.71. Oxygen saturation (sO2) and lumen diameters of blood vessels were measured in the retina. The average lumen diameter of the venules was 45.7\u00b113.6 \u03bcm, whereas the average lumen diameter of the arterioles was 31.5\u00b18.7 \u03bcm. The average arteriole sO2 was 98%, whereas the average venule sO2 was 58%.ConclusionsA high-resolution hyperspectral imaging system was developed and validated for retina imaging and measurement of blood vessels and oxygen saturation.<\/jats:p>","DOI":"10.1117\/1.jbo.31.3.036006","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:41:24Z","timestamp":1773837684000},"page":"1-19","source":"Crossref","is-referenced-by-count":1,"title":["Development and validation of a high-resolution hyperspectral imaging system for the retina"],"prefix":"10.1117","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9936-9654","authenticated-orcid":false,"given":"Minh H.","family":"Tran","sequence":"first","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelden","family":"Pruitt","sequence":"additional","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3739-1314","authenticated-orcid":false,"given":"Michelle","family":"Bryarly","sequence":"additional","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isioma","family":"Emordi","sequence":"additional","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arrsh","family":"Ali","sequence":"additional","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4352-5697","authenticated-orcid":false,"given":"Ling","family":"Ma","sequence":"additional","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9123-9484","authenticated-orcid":false,"given":"Baowei","family":"Fei","sequence":"additional","affiliation":[{"name":"University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Journal of Biomedical Optics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-31\/issue-03\/036006\/Development-and-validation-of-a-high-resolution-hyperspectral-imaging-system\/10.1117\/1.JBO.31.3.036006.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:15:46Z","timestamp":1790194546000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/journal-of-biomedical-optics\/volume-31\/issue-03\/036006\/Development-and-validation-of-a-high-resolution-hyperspectral-imaging-system\/10.1117\/1.JBO.31.3.036006.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2026,3,1]]}},"URL":"https:\/\/doi.org\/10.1117\/1.jbo.31.3.036006","relation":{},"ISSN":["1083-3668","1560-2281"],"issn-type":[{"value":"1083-3668","type":"print"},{"value":"1560-2281","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]}}}