{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:08:24Z","timestamp":1790197704475,"version":"4.1.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"03","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PARD 2024"},{"name":"RAEng Chair in Emerging Technologies","id":[{"id":"https:\/\/ror.org\/0008ahc55","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Medical Research Council","award":["MR\/V033964\/1"],"award-info":[{"award-number":["MR\/V033964\/1"]}],"id":[{"id":"https:\/\/ror.org\/03x94j517","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Alzheimer\u2019s Research UK","award":["PPG2023B-019"],"award-info":[{"award-number":["PPG2023B-019"]}],"id":[{"id":"https:\/\/ror.org\/02ymzm013","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Union\u2019s Horizon 2020","award":["H2020-ICT"],"award-info":[{"award-number":["H2020-ICT"]}]},{"name":"DEEPER","award":["101016787"],"award-info":[{"award-number":["101016787"]}]},{"name":"MINING","award":["101125498"],"award-info":[{"award-number":["101125498"]}]},{"name":"European Union\u2019s Horizon Europe","award":["101163003"],"award-info":[{"award-number":["101163003"]}]},{"name":"European Union - NextGenerationEU","award":["ECS00000035"],"award-info":[{"award-number":["ECS00000035"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"PNRR MUR - M4C2 - Investimento 1.5 - Avviso \u2018Ecosistemi dell\u2019Innovazione\u2019 CUP","award":["J33C22001220001"],"award-info":[{"award-number":["J33C22001220001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurophoton."],"published-print":{"date-parts":[[2025,9,23]]},"abstract":"<jats:p>SignificanceCurrent preclinical evaluation of Alzheimer\u2019s disease pathology in mouse models relies on post-mortem analyses, which hinders the development and optimization of therapeutic approaches. Although in vivo methods exist, monitoring amyloid plaque signals across multiple brain regions in freely behaving animals remains a significant challenge.AimWe aim to develop an optical approach to address this challenge.ApproachWe used flat and tapered optical fibers in an Alzheimer\u2019s mouse model.ResultsWe first confirmed that conventional flat fiber-based photometry can detect amyloid plaque signals across multiple brain regions under anesthesia after injecting a blood-brain-barrier-permeable tracer, Methoxy-X04. The depth profile of in vivo fluorescent signals is correlated with histological signals. A machine learning approach could distinguish between in vivo fluorescent signals of mice with and without amyloid plaques. Next, after validating the feasibility of depth-resolved fiber photometry ex vivo, we chronically implanted a tapered fiber to monitor amyloid plaque signals in freely behaving mice. After injecting Methoxy-X04, fluorescent signals increased in a depth-specific manner in Alzheimer\u2019s mice, but not in their wild-type littermates.ConclusionsOur approach expands the capabilities of fiber photometry to monitor molecular pathologies, such as amyloid plaques, even in a freely behaving condition.<\/jats:p>","DOI":"10.1117\/1.nph.12.3.035014","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T18:54:07Z","timestamp":1758653647000},"page":"1-16","source":"Crossref","is-referenced-by-count":3,"title":["Depth-resolved fiber photometry of amyloid plaque signals in freely behaving Alzheimer\u2019s disease mice"],"prefix":"10.1117","volume":"12","author":[{"given":"Nicole","family":"Byron","sequence":"first","affiliation":[{"name":"Univ. of Strathclyde"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0369-8788","authenticated-orcid":false,"given":"Niall","family":"McAlinden","sequence":"additional","affiliation":[{"name":"Univ. of Strathclyde"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Pisano","sequence":"additional","affiliation":[{"name":"Istituto Italiano di Tecnologia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2179-7883","authenticated-orcid":false,"given":"Marco","family":"Pisanello","sequence":"additional","affiliation":[{"name":"OptogeniX S.r.l."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Univ. of Strathclyde"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinzia","family":"Montinaro","sequence":"additional","affiliation":[{"name":"Istituto Italiano di Tecnologia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Mathieson","sequence":"additional","affiliation":[{"name":"Univ. of Strathclyde"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"De Vittorio","sequence":"additional","affiliation":[{"name":"IIstituto Italiano di Tecnologia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1489-7758","authenticated-orcid":false,"given":"Ferruccio","family":"Pisanello","sequence":"additional","affiliation":[{"name":"Istituto Italiano di Tecnologia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6796-411X","authenticated-orcid":false,"given":"Shuzo","family":"Sakata","sequence":"additional","affiliation":[{"name":"Univ.of Strathclyde"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Neurophotonics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/neurophotonics\/volume-12\/issue-03\/035014\/Depth-resolved-fiber-photometry-of-amyloid-plaque-signals-in-freely\/10.1117\/1.NPh.12.3.035014.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:38:45Z","timestamp":1790195925000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/neurophotonics\/volume-12\/issue-03\/035014\/Depth-resolved-fiber-photometry-of-amyloid-plaque-signals-in-freely\/10.1117\/1.NPh.12.3.035014.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,23]]},"references-count":0,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.1117\/1.nph.12.3.035014","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.04.08.647900","asserted-by":"object"}]},"ISSN":["2329-423X","2329-4248"],"issn-type":[{"value":"2329-423X","type":"print"},{"value":"2329-4248","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,23]]}}}