{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:59:30Z","timestamp":1790207970355,"version":"4.1.0"},"reference-count":0,"publisher":"SPIE-Intl Soc Optical Eng","issue":"S2","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01NS108472-04"],"award-info":[{"award-number":["R01NS108472-04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurophoton."],"published-print":{"date-parts":[[2025,8,12]]},"abstract":"<jats:p>SignificanceCapillaries are the critical site of vascular exchange with the local tissue, with continuous flow to meet the brain\u2019s unique and steep energetic demands. However, transient stalls in capillary flow have been observed and at elevated levels in preclinical models of disease. Systematic measurements have not been made to quantify the acute effects of individual capillary stalls on local oxygen.AimWe aim to quantify oxygen dynamics around capillary stalls as they occur in vivo.ApproachWe use high-resolution two-photon phosphorescent lifetime microscopy (2PLM) to monitor capillary flux and pO2 in the mouse cortex, allowing us to capture acute oxygen dynamics around capillary stalling.ResultsAll stalls cause rapid drops in intra-capillary oxygen that likely extend to local tissue based on estimates using the erythrocyte-associated transient (EAT). This includes a subset of capillaries, which reach critically hypoxic levels (&lt;10\u00a0mmHg), which could not be predicted by the capillaries\u2019 normal flux and oxygen levels, nor local vessel density or proximity to diving arterioles and venules.ConclusionOur findings indicate that a subset of capillary stalls reach extremely low local oxygen, resulting in transient hypoxia in the surrounding tissue. This reveals a new potential pathological mechanism due to stalled capillary flow.<\/jats:p>","DOI":"10.1117\/1.nph.12.s2.s22803","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T08:19:36Z","timestamp":1754986776000},"page":"1-13","source":"Crossref","is-referenced-by-count":1,"title":["Brief disruptions in capillary flow result in rapid onset of hypoxia"],"prefix":"10.1117","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4483-2971","authenticated-orcid":false,"given":"John T.","family":"Giblin","sequence":"first","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8071-3259","authenticated-orcid":false,"given":"Sreekanth","family":"Kura","sequence":"additional","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fclce","family":"K\u00fcreli","sequence":"additional","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Jiang","sequence":"additional","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7248-8728","authenticated-orcid":false,"given":"K\u0131v\u0131lc\u0131m","family":"K\u0131l\u0131\u00e7","sequence":"additional","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Charlestown, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sava","family":"Sakad\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Charlestown, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5143-3960","authenticated-orcid":false,"given":"Anna","family":"Devor","sequence":"additional","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4995-293X","authenticated-orcid":false,"given":"David A.","family":"Boas","sequence":"additional","affiliation":[{"name":"Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","container-title":["Neurophotonics"],"original-title":[],"link":[{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/neurophotonics\/volume-12\/issue-S2\/S22803\/Brief-disruptions-in-capillary-flow-result-in-rapid-onset-of\/10.1117\/1.NPh.12.S2.S22803.full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:01:15Z","timestamp":1790204475000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/journals\/neurophotonics\/volume-12\/issue-S2\/S22803\/Brief-disruptions-in-capillary-flow-result-in-rapid-onset-of\/10.1117\/1.NPh.12.S2.S22803.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,12]]},"references-count":0,"journal-issue":{"issue":"S2","published-online":{"date-parts":[[2025,6,1]]}},"URL":"https:\/\/doi.org\/10.1117\/1.nph.12.s2.s22803","relation":{},"ISSN":["2329-423X","2329-4248"],"issn-type":[{"value":"2329-423X","type":"print"},{"value":"2329-4248","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,12]]}}}