{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:40:27Z","timestamp":1792294827759,"version":"4.4.0"},"reference-count":34,"publisher":"SPIE","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,4]]},"DOI":"10.1117\/12.3081819","type":"proceedings-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T23:15:55Z","timestamp":1772666155000},"page":"12","source":"Crossref","is-referenced-by-count":0,"title":["Combined excitation scan-based hyperspectral imaging and digital deconvolution for the assessment of the kinetic and spatial distributions of cAMP signals"],"prefix":"10.1117","author":[{"given":"Naga","family":"Annamdevula","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Tang-Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Ledoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Kate","family":"Driver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliott","family":"Franklin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Britain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Rich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silas J.","family":"Leavesley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"189","reference":[{"key":"c1","doi-asserted-by":"publisher","DOI":"10.1038\/42264"},{"key":"c2","doi-asserted-by":"publisher","DOI":"10.1038\/384166a0"},{"key":"c3","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90237-6"},{"key":"c4","doi-asserted-by":"publisher","DOI":"10.1146\/neuro.1989.12.issue-1"},{"key":"c5","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"c6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400302200"},{"key":"c7","first-page":"e0122513","article-title":"Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity,\u201d PLoS On","volume":"e10","author":"Klarenbeek","year":"2015"},{"key":"c8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400290"},{"key":"c9","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15031"},{"key":"c10","doi-asserted-by":"publisher","DOI":"10.1038\/71345"},{"key":"c11","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20220223"},{"key":"c12","first-page":"16513","article-title":"Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments","volume-title":"Proc. Natl. Acad. Sci. USA","volume":"101","author":"DiPilato","year":"2004"},{"key":"c13","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2022.07.007"},{"key":"c14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.02.011"},{"key":"c15","doi-asserted-by":"publisher","DOI":"10.1042\/BST20190245"},{"key":"c16","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.v69a:8"},{"key":"c17","doi-asserted-by":"publisher","DOI":"10.1042\/BST20230352"},{"key":"c18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188789"},{"key":"c19","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.4.046010"},{"key":"c20","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.v5.1"},{"key":"c21","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.v93.10"},{"key":"c22","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201600227"},{"key":"c23","first-page":"10.3791\/59448","article-title":"Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals","volume":"150","author":"Deal","year":"2019","journal-title":"J. Vis. Exp."},{"key":"c24","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.24.2.021207"},{"key":"c25","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.v13.2"},{"key":"c26","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.21.10.104003"},{"key":"c27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0284394"},{"key":"c28","first-page":"13049","article-title":"A uniform extracellular stimulus triggers distinct cAMP signals in different compartments of a simple cell","volume-title":"Proceedings of the National Academy of Sciences","volume":"98","author":"Rich","year":"2001"},{"key":"c29","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000134629.84732.11"},{"key":"c30","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000218493.09370.8e"},{"key":"c31","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.124891"},{"key":"c32","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00332"},{"key":"c33","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00361.2011"},{"key":"c34","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200605050"}],"event":{"name":"Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXIII","location":"San Francisco, United States","start":{"date-parts":[[2026,1,17]]},"end":{"date-parts":[[2026,1,23]]}},"container-title":["Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXIII"],"original-title":[],"deposited":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:03:14Z","timestamp":1792292594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.spiedigitallibrary.org\/conference-proceedings-of-spie\/13857\/3081819\/Combined-excitation-scan-based-hyperspectral-imaging-and-digital-deconvolution-for\/10.1117\/12.3081819.full"}},"subtitle":[],"editor":[{"given":"Thomas G.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]},{"given":"Tony","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]},{"given":"Laura","family":"Waller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,3,4]]},"references-count":34,"URL":"https:\/\/doi.org\/10.1117\/12.3081819","relation":{},"subject":[],"published":{"date-parts":[[2026,3,4]]}}}