{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:55:17Z","timestamp":1788580517883,"version":"build-2803163510"},"reference-count":46,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":1127,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["dmd.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Drug Metabolism and Disposition"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1124\/dmd.121.000559","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T15:50:12Z","timestamp":1632239412000},"page":"1038-1046","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"title":["Ontogeny of Small Intestinal Drug Transporters and Metabolizing Enzymes Based on Targeted Quantitative Proteomics"],"prefix":"10.1016","volume":"49","author":[{"given":"M\u00e1rton","family":"Kiss","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Mbasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Nicola\u00ef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Barnouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apoorva","family":"Kotian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam G.","family":"Mooij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nico","family":"Kist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene M.H.","family":"Wijnen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Lena","family":"Ungell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Cutler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans G.M.","family":"Russel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia N.","family":"de Wildt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/dmd.121.000559_bib1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1021\/acs.molpharmaceut.7b00772","article-title":"High expression of UGT1A1\/1A6 in monkey small intestine: comparison of protein expression levels of cytochromes P450, UDP-glucuronosyltransferases, and transporters in small intestine of cynomolgus monkey and human","volume":"15","author":"Akazawa","year":"2018","journal-title":"Mol Pharm"},{"key":"10.1124\/dmd.121.000559_bib2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/mnfr.201600874","article-title":"Wholegrain oat diet changes the expression of genes associated with intestinal bile acid transport","volume":"61","author":"Andersson","year":"2017","journal-title":"Mol Nutr Food Res"},{"key":"10.1124\/dmd.121.000559_bib3","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1111\/bcp.12268","article-title":"Formulations for children: problems and solutions","volume":"79","author":"Batchelor","year":"2015","journal-title":"Br J Clin Pharmacol"},{"key":"10.1124\/dmd.121.000559_bib4","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1124\/dmd.117.076463","article-title":"Age-dependent protein abundance of cytosolic alcohol and aldehyde dehydrogenases in human liver","volume":"45","author":"Bhatt","year":"2017","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1002\/cpt.1109","article-title":"Age- and genotype-dependent variability in the protein abundance and activity of six major uridine diphosphate-glucuronosyltransferases in human liver","volume":"105","author":"Bhatt","year":"2019","journal-title":"Clin Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib6","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1124\/dmd.116.072652","article-title":"Age-dependent absolute abundance of hepatic carboxylesterases (CES1 and CES2) by LC-MS\/MS proteomics: application to PBPK modeling of oseltamivir in vivo pharmacokinetics in infants","volume":"45","author":"Boberg","year":"2017","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib7","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1124\/dmd.109.029900","article-title":"Identification of human UGT2B7 as the major isoform involved in the O-glucuronidation of chloramphenicol","volume":"38","author":"Chen","year":"2010","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib8","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1124\/mol.105.020792","article-title":"Transactivation of rat apical sodium-dependent bile acid transporter and increased bile acid transport by 1alpha,25-dihydroxyvitamin D3 via the vitamin D receptor","volume":"69","author":"Chen","year":"2006","journal-title":"Mol Pharmacol"},{"key":"10.1124\/dmd.121.000559_bib9","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.tox.2015.02.007","article-title":"Ontogenic expression of human carboxylesterase-2 and cytochrome P450 3A4 in liver and duodenum: postnatal surge and organ-dependent regulation","volume":"330","author":"Chen","year":"2015","journal-title":"Toxicology"},{"key":"10.1124\/dmd.121.000559_bib10","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1002\/cpt.1516","article-title":"A comprehensive analysis of ontogeny of renal drug transporters: mRNA analyses, quantitative proteomics, and localization","volume":"106","author":"Cheung","year":"2019","journal-title":"Clin Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib11","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1021\/acs.molpharmaceut.8b00941","article-title":"Quantification of proteins involved in drug metabolism and disposition in the human liver using label-free global proteomics","volume":"16","author":"Couto","year":"2019","journal-title":"Mol Pharm"},{"key":"10.1124\/dmd.121.000559_bib12","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1124\/dmd.119.089656","article-title":"Quantitative proteomics of clinically relevant drug-metabolizing enzymes and drug transporters and their intercorrelations in the human small intestine","volume":"48","author":"Couto","year":"2020","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib13","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1002\/cpt.967","article-title":"Protein abundance of clinically relevant drug-metabolizing enzymes in the human liver and intestine: a comparative analysis in paired tissue specimens","volume":"104","author":"Drozdzik","year":"2018","journal-title":"Clin Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib14","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1002\/cpt.1301","article-title":"Protein abundance of clinically relevant drug transporters in the human liver and intestine: a comparative analysis in paired tissue specimens","volume":"105","author":"Drozdzik","year":"2019","journal-title":"Clin Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib15","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1021\/mp500330y","article-title":"Protein abundance of clinically relevant multidrug transporters along the entire length of the human intestine","volume":"11","author":"Drozdzik","year":"2014","journal-title":"Mol Pharm"},{"key":"10.1124\/dmd.121.000559_bib16","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1124\/dmd.105.005611","article-title":"Localization and mRNA expression of CYP3A and P-glycoprotein in human duodenum as a function of age","volume":"33","author":"Fakhoury","year":"2005","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib17","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1111\/bcp.12540","article-title":"Paediatric prescribing: why children are not small adults","volume":"79","author":"Ferro","year":"2015","journal-title":"Br J Clin Pharmacol"},{"key":"10.1124\/dmd.121.000559_bib18","doi-asserted-by":"crossref","first-page":"5024","DOI":"10.1073\/pnas.0913290107","article-title":"Developmental hyperbilirubinemia and CNS toxicity in mice humanized with the UDP glucuronosyltransferase 1 (UGT1) locus","volume":"107","author":"Fujiwara","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/dmd.121.000559_bib19","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jpba.2013.07.031","article-title":"LC-MS\/MS-based quantification of clinically relevant intestinal uptake and efflux transporter proteins","volume":"85","author":"Gr\u00f6er","year":"2013","journal-title":"J Pharm Biomed Anal"},{"key":"10.1124\/dmd.121.000559_bib20","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/bdd.2070","article-title":"Optimization of intestinal microsomal preparation in the rat: a systematic approach to assess the influence of various methodologies on metabolic activity and scaling factors","volume":"38","author":"Hatley","year":"2017","journal-title":"Biopharm Drug Dispos"},{"key":"10.1124\/dmd.121.000559_bib21","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/nrd3028","article-title":"Membrane transporters in drug development","volume":"9","author":"Giacomini","year":"2010","journal-title":"Nat Rev Drug Discov"},{"key":"10.1124\/dmd.121.000559_bib22","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1046\/j.1365-2125.2001.01370.x","article-title":"Enterocytic CYP3A4 in a paediatric population: developmental changes and the effect of coeliac disease and cystic fibrosis","volume":"51","author":"Johnson","year":"2001","journal-title":"Br J Clin Pharmacol"},{"key":"10.1124\/dmd.121.000559_bib23","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1007\/s11095-008-9532-4","article-title":"Quantitative atlas of membrane transporter proteins: development and application of a highly sensitive simultaneous LC\/MS\/MS method combined with novel in-silico peptide selection criteria","volume":"25","author":"Kamiie","year":"2008","journal-title":"Pharm Res"},{"key":"10.1124\/dmd.121.000559_bib24","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0022-3476(81)80670-1","article-title":"Pharmacokinetics of chloramphenicol and chloramphenicol succinate in infants and children","volume":"98","author":"Kauffman","year":"1981","journal-title":"J Pediatr"},{"key":"10.1124\/dmd.121.000559_bib25","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1007\/s10735-011-9363-1","article-title":"Differential expression of ABC transporters (MDR1, MRP1, BCRP) in developing human embryos","volume":"42","author":"Konieczna","year":"2011","journal-title":"J Mol Histol"},{"key":"10.1124\/dmd.121.000559_bib26","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.dmpk.2019.01.001","article-title":"Proteomic analysis of small intestinal epithelial cells in antibiotic-treated mice: changes in drug transporters and metabolizing enzymes","volume":"34","author":"Kuno","year":"2019","journal-title":"Drug Metab Pharmacokinet"},{"key":"10.1124\/dmd.121.000559_bib27","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1124\/dmd.119.086462","article-title":"Ontogeny of hepatic sulfotransferases and prediction of age-dependent fractional contribution of sulfation in acetaminophen metabolism","volume":"47","author":"Ladumor","year":"2019","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib28","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1208\/s12248-019-0359-1","article-title":"Optimized renal transporter quantification by using aquaporin 1 and aquaporin 2 as anatomical markers: application in characterizing the ontogeny of renal transporters and its correlation with hepatic transporters in paired human samples","volume":"21","author":"Li","year":"2019","journal-title":"AAPS J"},{"key":"10.1124\/dmd.121.000559_bib29","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/j.ejca.2007.01.002","article-title":"Standard operating procedure for the collection of fresh frozen tissue samples","volume":"43","author":"Mager","year":"2007","journal-title":"Eur J Cancer"},{"key":"10.1124\/dmd.121.000559_bib30","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1124\/dmd.115.068809","article-title":"Human intestinal PEPT1 transporter expression and localization in preterm and term infants","volume":"44","author":"Mooij","year":"2016","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib31","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1124\/dmd.114.056929","article-title":"Ontogeny of human hepatic and intestinal transporter gene expression during childhood: age matters","volume":"42","author":"Mooij","year":"2014","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib32","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1023\/A:1018890106045","article-title":"Evaluation of viability of excised rat intestinal segments in the Ussing chamber: investigation of morphology, electrical parameters, and permeability characteristics","volume":"16","author":"Polentarutti","year":"1999","journal-title":"Pharm Res"},{"key":"10.1124\/dmd.121.000559_bib33","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/cpt.409","article-title":"Ontogeny of hepatic drug transporters as quantified by LC-MS\/MS proteomics","volume":"100","author":"Prasad","year":"2016","journal-title":"Clin Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib34","unstructured":"Ritschel WA (1980) Handbook of Basic Pharmacokinetics, Drug Intelligence Publications, Hamilton, IL 62341."},{"key":"10.1124\/dmd.121.000559_bib35","first-page":"296","article-title":"Use of body surface area for dosing of vancomycin","volume":"24","author":"Sawrey","year":"2019","journal-title":"J Pediatr Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib36","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s00280-012-1981-0","article-title":"Uridine diphosphate glucuronosyl transferase 1 family polypeptide A1 gene (UGT1A1) polymorphisms are associated with toxicity and efficacy in irinotecan monotherapy for refractory pancreatic cancer","volume":"71","author":"Takahara","year":"2013","journal-title":"Cancer Chemother Pharmacol"},{"key":"10.1124\/dmd.121.000559_bib37","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1124\/dmd.116.072231","article-title":"Regional expression levels of drug transporters and metabolizing enzymes along the pig and human intestinal tract and comparison with Caco-2 cells","volume":"45","author":"Vaessen","year":"2017","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.121.000559_bib38","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ejps.2018.08.042","article-title":"Proteomics of human liver membrane transporters: a focus on fetuses and newborn infants","volume":"124","author":"van Groen","year":"2018","journal-title":"Eur J Pharm Sci"},{"key":"10.1124\/dmd.121.000559_bib39","first-page":"1063","article-title":"Multidrug resistance gene (P-glycoprotein) expression in the human fetus","volume":"141","author":"van Kalken","year":"1992","journal-title":"Am J Pathol"},{"key":"10.1124\/dmd.121.000559_bib40","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s00418-020-01884-8","article-title":"Developmental patterns in human blood-brain barrier and blood-cerebrospinal fluid barrier ABC drug transporter expression","volume":"154","author":"Verscheijden","year":"2020","journal-title":"Histochem Cell Biol"},{"key":"10.1124\/dmd.121.000559_bib41","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1056\/NEJM196004212621601","article-title":"Chloramphenicol in the newborn infant. A physiologic explanation of its toxicity when given in excessive doses","volume":"262","author":"Weiss","year":"1960","journal-title":"N Engl J Med"},{"key":"10.1124\/dmd.121.000559_bib42","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/0016-5085(88)90421-0","article-title":"Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms","volume":"94","author":"West","year":"1988","journal-title":"Gastroenterology"},{"key":"10.1124\/dmd.121.000559_bib43","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.apsb.2016.07.012","article-title":"An update on the role of intestinal cytochrome P450 enzymes in drug disposition","volume":"6","author":"Xie","year":"2016","journal-title":"Acta Pharm Sin B"},{"key":"10.1124\/dmd.121.000559_bib44","doi-asserted-by":"crossref","first-page":"9723","DOI":"10.1073\/pnas.1919410117","article-title":"Sample multiplexing for targeted pathway proteomics in aging mice","volume":"117","author":"Yu","year":"2020","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/dmd.121.000559_bib45","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1002\/cpt.1112","article-title":"Transporters in drug development: 2018 ITC recommendations for transporters of emerging clinical importance","volume":"104","author":"Zamek-Gliszczynski","year":"2018","journal-title":"Clin Pharmacol Ther"},{"key":"10.1124\/dmd.121.000559_bib46","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1124\/dmd.120.090738","article-title":"Regional proteomic quantification of clinically relevant non-cytochrome P450 enzymes along the human small intestine","volume":"48","author":"Zhang","year":"2020","journal-title":"Drug Metab Dispos"}],"container-title":["Drug Metabolism and Disposition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0090955624080103?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0090955624080103?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/dmd.121.000559","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T18:04:40Z","timestamp":1740765880000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0090955624080103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":46,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["S0090955624080103"],"URL":"https:\/\/doi.org\/10.1124\/dmd.121.000559","relation":{},"ISSN":["0090-9556"],"issn-type":[{"value":"0090-9556","type":"print"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ontogeny of Small Intestinal Drug Transporters and Metabolizing Enzymes Based on Targeted Quantitative Proteomics","name":"articletitle","label":"Article Title"},{"value":"Drug Metabolism and Disposition","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/dmd.121.000559","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 by The Author(s)","name":"copyright","label":"Copyright"}]}}