{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:42:14Z","timestamp":1787244134687,"version":"build-2736575974"},"reference-count":30,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["dmd.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Drug Metabolism and Disposition"],"published-print":{"date-parts":[[2022,9]]},"DOI":"10.1124\/dmd.122.000900","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T12:01:29Z","timestamp":1661860889000},"page":"1182-1189","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["High-Throughput Production of Diverse Xenobiotic Metabolites with Cytochrome P450\u2013Transduced Huh7 Hepatoma Cell Lines"],"prefix":"10.1016","volume":"50","author":[{"given":"Choon-myung","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken H.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant","family":"Singer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean P.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4273-2261","authenticated-orcid":false,"given":"Edward T.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/dmd.122.000900_bib1","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1021\/acs.analchem.8b04698","article-title":"Structure Annotation of All Mass Spectra in Untargeted Metabolomics","volume":"91","author":"Bla\u017eenovi\u0107","year":"2019","journal-title":"Anal Chem"},{"key":"10.1124\/dmd.122.000900_bib2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1124\/dmd.116.070763","article-title":"Prevalence of non-cytochrome p450-mediated metabolism in food and drug administration-approved oral and intravenous drugs: 2006-2015","volume":"44","author":"Cerny","year":"2016","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib3","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1124\/dmd.119.089961","article-title":"Nitric oxide mediated degradation of CYP2A6 via the ubiquitin-proteasome pathway in human hepatoma cells","volume":"48","author":"Cerrone","year":"2020","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib4","first-page":"399","article-title":"Coexpression of cytochrome P4502A6 and human NADPH-P450 oxidoreductase in the baculovirus system","volume":"25","author":"Chen","year":"1997","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1080\/00498250802613620","article-title":"Characterization of increased drug metabolism activity in dimethyl sulfoxide (DMSO)-treated Huh7 hepatoma cells","volume":"39","author":"Choi","year":"2009","journal-title":"Xenobiotica"},{"key":"10.1124\/dmd.122.000900_bib6","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0009-2797(85)80103-4","article-title":"Xenobiotic metabolism and mutation in a human lymphoblastoid cell line","volume":"53","author":"Crespi","year":"1985","journal-title":"Chem Biol Interact"},{"key":"10.1124\/dmd.122.000900_bib7","first-page":"97","article-title":"The development of a panel of human cell lines expressing specific human cytochrome P450 cDNAs","volume":"340B","author":"Crespi","year":"1990","journal-title":"Prog Clin Biol Res"},{"key":"10.1124\/dmd.122.000900_bib8","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13321-018-0324-5","article-title":"BioTransformer: a comprehensive computational tool for small molecule metabolism prediction and metabolite identification","volume":"11","author":"Djoumbou-Feunang","year":"2019","journal-title":"J Cheminform"},{"key":"10.1124\/dmd.122.000900_bib9","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1124\/dmd.32.7.699","article-title":"Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes","volume":"32","author":"Donato","year":"2004","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib10","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1002\/bdd.374","article-title":"Rapid determination of enzyme activities of recombinant human cytochromes P450, human liver microsomes and hepatocytes","volume":"24","author":"Ghosal","year":"2003","journal-title":"Biopharm Drug Dispos"},{"key":"10.1124\/dmd.122.000900_bib11","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/S0026-895X(24)12046-9","article-title":"CYP2B6 mediates the in vitro hydroxylation of bupropion: potential drug interactions with other antidepressants","volume":"28","author":"Hesse","year":"2000","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib12","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1002\/j.1552-4604.1997.tb04361.x","article-title":"Pharmacokinetics of bupropion and its metabolites in cigarette smokers versus nonsmokers","volume":"37","author":"Hsyu","year":"1997","journal-title":"J Clin Pharmacol"},{"key":"10.1124\/dmd.122.000900_bib13","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.toxrep.2015.11.009","article-title":"Sequencing the exposome: A call to action","volume":"3","author":"Jones","year":"2016","journal-title":"Toxicol Rep"},{"key":"10.1124\/dmd.122.000900_bib14","first-page":"443","article-title":"Pazopanib: the newest tyrosine kinase inhibitor for the treatment of advanced or metastatic renal cell carcinoma","volume":"71","author":"Keisner","year":"2011","journal-title":"Drugs"},{"key":"10.1124\/dmd.122.000900_bib15","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1124\/molpharm.120.000020","article-title":"Tyrosine nitration contributes to nitric oxide-stimulated degradation of CYP2B6","volume":"98","author":"Lee","year":"2020","journal-title":"Mol Pharmacol"},{"key":"10.1124\/dmd.122.000900_bib16","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1124\/jpet.105.090928","article-title":"Enantiomer\/enantiomer interactions between the S- and R- isomers of omeprazole in human cytochrome P450 enzymes: major role of CYP2C19 and CYP3A4","volume":"315","author":"Li","year":"2005","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/dmd.122.000900_bib17","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1124\/jpet.300.2.399","article-title":"Amodiaquine clearance and its metabolism to N-desethylamodiaquine is mediated by CYP2C8: a new high affinity and turnover enzyme-specific probe substrate","volume":"300","author":"Li","year":"2002","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/dmd.122.000900_bib18","doi-asserted-by":"crossref","first-page":"5418","DOI":"10.1038\/s41467-021-25698-x","article-title":"Large scale enzyme based xenobiotic identification for exposomics","volume":"12","author":"Liu","year":"2021","journal-title":"Nat Commun"},{"key":"10.1124\/dmd.122.000900_bib19","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1186\/s12936-019-2860-5","article-title":"A non-lethal malarial infection results in reduced drug metabolizing enzyme expression and drug clearance in mice","volume":"18","author":"Mimche","year":"2019","journal-title":"Malar J"},{"key":"10.1124\/dmd.122.000900_bib20","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2012.0431","article-title":"Human cytochromes P450 in health and disease","volume":"368","author":"Nebert","year":"2013","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"10.1124\/dmd.122.000900_bib21","doi-asserted-by":"crossref","first-page":"3241","DOI":"10.1042\/BCJ20170459","article-title":"Nitric oxide stimulates cellular degradation of human CYP51A1, the highly conserved lanosterol 14\u03b1-demethylase","volume":"474","author":"Park","year":"2017","journal-title":"Biochem J"},{"key":"10.1124\/dmd.122.000900_bib22","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0300-483X(99)00200-0","article-title":"CYP2A6: a human coumarin 7-hydroxylase","volume":"144","author":"Pelkonen","year":"2000","journal-title":"Toxicology"},{"key":"10.1124\/dmd.122.000900_bib23","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1002\/rcm.3117","article-title":"Identification of bupropion urinary metabolites by liquid chromatography\/mass spectrometry","volume":"21","author":"Petsalo","year":"2007","journal-title":"Rapid Commun Mass Spectrom"},{"key":"10.1124\/dmd.122.000900_bib24","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1186\/1471-2105-11-395","article-title":"MZmine 2: modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data","volume":"11","author":"Pluskal","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"10.1124\/dmd.122.000900_bib25","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1124\/dmd.114.058636","article-title":"Regioselective hydroxylation of omeprazole enantiomers by bacterial CYP102A1 mutants","volume":"42","author":"Ryu","year":"2014","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib26","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1021\/acs.analchem.6b01214","article-title":"xMSannotator: an R package for network-based annotation of high-resolution metabolomics data","volume":"89","author":"Uppal","year":"2017","journal-title":"Anal Chem"},{"key":"10.1124\/dmd.122.000900_bib27","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1021\/acs.chemrestox.6b00179","article-title":"Computational metabolomics: a framework for the million metabolome","volume":"29","author":"Uppal","year":"2016","journal-title":"Chem Res Toxicol"},{"key":"10.1124\/dmd.122.000900_bib28","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S0090-9556(24)15373-1","article-title":"CYP2C19 participates in tolbutamide hydroxylation by human liver microsomes","volume":"28","author":"Wester","year":"2000","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.122.000900_bib29","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/S0022-3565(24)37072-7","article-title":"Different contributions of cytochrome P450 2C19 and 3A4 in the oxidation of omeprazole by human liver microsomes: effects of contents of these two forms in individual human samples","volume":"283","author":"Yamazaki","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/dmd.122.000900_bib30","first-page":"1514","article-title":"Comparative contribution to dextromethorphan metabolism by cytochrome P450 isoforms in vitro: can dextromethorphan be used as a dual probe for both CTP2D6 and CYP3A activities?","volume":"29","author":"Yu","year":"2001","journal-title":"Drug Metab Dispos"}],"container-title":["Drug Metabolism and Disposition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0090955624008663?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0090955624008663?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/dmd.122.000900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T03:57:25Z","timestamp":1739937445000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0090955624008663"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["S0090955624008663"],"URL":"https:\/\/doi.org\/10.1124\/dmd.122.000900","relation":{},"ISSN":["0090-9556"],"issn-type":[{"value":"0090-9556","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-Throughput Production of Diverse Xenobiotic Metabolites with Cytochrome P450\u2013Transduced Huh7 Hepatoma Cell Lines","name":"articletitle","label":"Article Title"},{"value":"Drug Metabolism and Disposition","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/dmd.122.000900","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 by The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}