{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:28:02Z","timestamp":1792898882824,"version":"4.5.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["dmd.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Drug Metabolism and Disposition"],"published-print":{"date-parts":[[2003,8]]},"DOI":"10.1124\/dmd.31.8.1035","type":"journal-article","created":{"date-parts":[[2003,7,16]],"date-time":"2003-07-16T16:26:32Z","timestamp":1058372792000},"page":"1035-1042","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":712,"title":["COMPARISON OF PRIMARY HUMAN HEPATOCYTES AND HEPATOMA CELL LINE HEPG2 WITH REGARD TO THEIR BIOTRANSFORMATION PROPERTIES"],"prefix":"10.1016","volume":"31","author":[{"given":"Stefan","family":"Wilkening","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Stahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustinus","family":"Bader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/dmd.31.8.1035_bib1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1089\/dna.1998.17.231","article-title":"Quantification of multiple human cytochrome P450 mRNA molecules using competitive reverse transcriptase-PCR.","volume":"17","author":"Andersen","year":"1998","journal-title":"DNA Cell Biol"},{"key":"10.1124\/dmd.31.8.1035_bib2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1021\/bp00015a007","article-title":"A stable long-term hepatocyte culture system for studies of physiologic processes: cytokine stimulation of the acute phase response in rat and human hepatocytes.","volume":"8","author":"Bader","year":"1992","journal-title":"Biotechnol Prog"},{"key":"10.1124\/dmd.31.8.1035_bib3","doi-asserted-by":"crossref","first-page":"623","DOI":"10.3109\/00498259409043265","article-title":"Use of organotypical cultures of primary hepatocytes to analyse drug biotransformation in man and animals.","volume":"24","author":"Bader","year":"1994","journal-title":"Xenobiotica"},{"key":"10.1124\/dmd.31.8.1035_bib4","doi-asserted-by":"crossref","first-page":"4315","DOI":"10.1074\/jbc.M008895200","article-title":"Adenovirus-mediated overexpression of catalase in the cytosolic or mitochondrial compartment protects against cytochrome P450 2E1-dependent toxicity in HepG2 cells.","volume":"276","author":"Bai","year":"2001","journal-title":"J Biol Chem"},{"key":"10.1124\/dmd.31.8.1035_bib5","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1089\/dna.1990.9.193","article-title":"Human arylamine N-acetyltransferase genes: isolation, chromosomal localization and functional expression.","volume":"9","author":"Blum","year":"1990","journal-title":"DNA Cell Biol"},{"key":"10.1124\/dmd.31.8.1035_bib6","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0027-5107(97)00296-0","article-title":"The pitfall of detoxifying enzymes.","volume":"402","author":"Cantelli-Forti","year":"1998","journal-title":"Mutat Res"},{"key":"10.1124\/dmd.31.8.1035_bib7","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1093\/carcin\/19.11.1969","article-title":"Metabolism of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine by human cytochrome P4501A1, P4501A2 and P4501B1.","volume":"19","author":"Crofts","year":"1998","journal-title":"Carcinogenesis"},{"key":"10.1124\/dmd.31.8.1035_bib8","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/0092-8674(91)90531-3","article-title":"A liver-enriched transcriptional activator protein, LAP and a transcriptional inhibitory protein, LIP, are translated from the same mRNA.","volume":"67","author":"Descombes","year":"1991","journal-title":"Cell"},{"key":"10.1124\/dmd.31.8.1035_bib9","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1080\/02652039609374439","article-title":"Dietary intakes of nitrate, nitrite and NDMA in the Finnish Mobile Clinic Health Examination Survey.","volume":"13","author":"Dich","year":"1996","journal-title":"Food Addit Contam"},{"key":"10.1124\/dmd.31.8.1035_bib10","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1006\/abio.1993.1381","article-title":"A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates.","volume":"213","author":"Donato","year":"1993","journal-title":"Anal Biochem"},{"key":"10.1124\/dmd.31.8.1035_bib11","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0300-483X(99)00215-2","article-title":"Bioflavonoids: selective substrates and inhibitors for cytochrome P450 CYP1A and CYP1B1.","volume":"144","author":"Doostdar","year":"2000","journal-title":"Toxicology"},{"key":"10.1124\/dmd.31.8.1035_bib12","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1096\/fasebj.3.2.2914628","article-title":"Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration.","volume":"3","author":"Dunn","year":"1989","journal-title":"FASEB J"},{"key":"10.1124\/dmd.31.8.1035_bib13","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1083\/jcb.54.3.626","article-title":"Collagen substrata for studies on cell behavior.","volume":"54","author":"Elsdale","year":"1972","journal-title":"J Cell Biol"},{"key":"10.1124\/dmd.31.8.1035_bib14","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1097\/00008571-199612000-00002","article-title":"Contribution of human cytochrome P450 to benzo[a]pyrene and benzo[a]pyrene-7,8-dihydrodiol metabolism, as predicted from heterologous expression in yeast.","volume":"6","author":"Gautier","year":"1996","journal-title":"Pharmacogenetics"},{"key":"10.1124\/dmd.31.8.1035_bib15","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1124\/jpet.300.2.355","article-title":"The ontogeny of human drug-metabolizing enzymes: phase I oxidative enzymes.","volume":"300","author":"Hines","year":"2002","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/dmd.31.8.1035_bib16","first-page":"125","article-title":"N-Nitrosodimethylamine.","volume":"17","year":"1978","journal-title":"IARC Monogr Eval Carcinog Risk Chem Hum"},{"key":"10.1124\/dmd.31.8.1035_bib17","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/0006-2952(96)00052-4","article-title":"Multiple forms of human P450 expressed in Saccharomyces cerevisiae. Systematic characterization and comparison with those of the rat.","volume":"51","author":"Imaoka","year":"1996","journal-title":"Biochem Pharmacol"},{"key":"10.1124\/dmd.31.8.1035_bib18","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0027-5107(97)00032-8","article-title":"Carcinogenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in the rat.","volume":"376","author":"Ito","year":"1997","journal-title":"Mutat Res"},{"key":"10.1124\/dmd.31.8.1035_bib19","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1093\/carcin\/17.3.435","article-title":"Autoregulation of human CYP1A1 gene promotor activity in HepG2 and MCF-7 cells.","volume":"17","author":"Jorgensen","year":"1996","journal-title":"Carcinogenesis"},{"key":"10.1124\/dmd.31.8.1035_bib20","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1053\/jhep.2001.22176","article-title":"Cytochrome P450 regulation by hepatocyte nuclear factor 4 in human hepatocytes: a study using adenovirus-mediated anti-sense targeting.","volume":"33","author":"Jover","year":"2001","journal-title":"Hepatology"},{"key":"10.1124\/dmd.31.8.1035_bib21","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/978-1-4615-4853-9_12","article-title":"Food heating and the formation of heterocyclic aromatic amine and polycyclic aromatic hydrocarbon mutagens\/carcinogens.","volume":"459","author":"Knize","year":"1999","journal-title":"Adv Exp Med Biol"},{"key":"10.1124\/dmd.31.8.1035_bib22","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1038\/15248","article-title":"The functional relevance of passenger leukocytes and microchimerism for heart allograft acceptance in the rat.","volume":"5","author":"Ko","year":"1999","journal-title":"Nat Med"},{"key":"10.1124\/dmd.31.8.1035_bib23","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1016\/S0006-2952(01)00763-8","article-title":"Involvement of a post-transcriptional mechanism in the inhibition of CYP1A1 expression by resveratrol in breast cancer cells.","volume":"62","author":"Lee","year":"2001","journal-title":"Biochem Pharmacol"},{"key":"10.1124\/dmd.31.8.1035_bib24","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1006\/abbi.2000.2115","article-title":"The human cytochrome P450 1A1 mRNA is rapidly degraded in HepG2 cells.","volume":"384","author":"Lekas","year":"2000","journal-title":"Arch Biochem Biophys"},{"key":"10.1124\/dmd.31.8.1035_bib25","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1093\/mutage\/6.5.399","article-title":"Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens\/carcinogens.","volume":"6","author":"Natarajan","year":"1991","journal-title":"Mutagenesis"},{"key":"10.1124\/dmd.31.8.1035_bib26","series-title":"Toxicology the Basic Science of Poisons.","author":"Parkinson","year":"1996"},{"key":"10.1124\/dmd.31.8.1035_bib27","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1016\/0006-2952(92)90694-E","article-title":"DT-diaphorase and cancer chemotherapy.","volume":"43","author":"Riley","year":"1992","journal-title":"Biochem Pharmacol"},{"key":"10.1124\/dmd.31.8.1035_bib28","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1080\/00498250210128675","article-title":"Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells.","volume":"32","author":"Rodriguez-Antona","year":"2002","journal-title":"Xenobiotica"},{"key":"10.1124\/dmd.31.8.1035_bib29","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1006\/abbi.2000.1697","article-title":"Quantitative RT-PCR measurement of human cytochrome P-450s: application to drug induction studies.","volume":"376","author":"Rodriguez-Antona","year":"2000","journal-title":"Arch Biochem Biophys"},{"key":"10.1124\/dmd.31.8.1035_bib30","series-title":"Molecular Cloning: A Laboratory Manual.","author":"Sambrook","year":"2001"},{"key":"10.1124\/dmd.31.8.1035_bib31","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1097\/00008571-199402000-00002","article-title":"Selective expression of cytochrome P450 CYP3A mRNAs in embryonic and adult human liver.","volume":"4","author":"Schuetz","year":"1994","journal-title":"Pharmacogenetics"},{"key":"10.1124\/dmd.31.8.1035_bib32","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0091-679X(08)61797-5","article-title":"Preparation of isolated rat liver cells.","volume":"13","author":"Seglen","year":"1976","journal-title":"Methods Cell Biol"},{"key":"10.1124\/dmd.31.8.1035_bib33","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/252326a0","article-title":"Metabolic activation of benzo-(a)pyrene proceeds by a diol-epoxide.","volume":"252","author":"Sims","year":"1974","journal-title":"Nature (Lond)"},{"key":"10.1124\/dmd.31.8.1035_bib34","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0014-4827(88)90265-0","article-title":"A simple technique for quantitation of low levels of DNA damage in individual cells.","volume":"175","author":"Singh","year":"1988","journal-title":"Exp Cell Res"},{"key":"10.1124\/dmd.31.8.1035_bib35","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1124\/mol.52.2.212","article-title":"Differential expression of the UGT1A locus in human liver, biliary and gastric tissue: identification of UGT1A7 and UGT1A10 transcripts in extrahepatic tissue.","volume":"52","author":"Strassburg","year":"1997","journal-title":"Mol Pharmacol"},{"key":"10.1124\/dmd.31.8.1035_bib36","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S1383-5718(99)00050-9","article-title":"Single-cell gel electrophoresis assays with human-derived hepatoma (Hep G2) cells.","volume":"441","author":"Uhl","year":"1999","journal-title":"Mutat Res"},{"key":"10.1124\/dmd.31.8.1035_bib37","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1152\/physiolgenomics.2000.2.3.143","article-title":"Comparison of human adult and fetal expression and identification of 535 housekeeping\/maintenance genes.","volume":"2","author":"Warrington","year":"2000","journal-title":"Physiol Genomics"},{"key":"10.1124\/dmd.31.8.1035_bib38","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0090-9556(25)08140-1","article-title":"Drug metabolism by isolated fetal human hepatocytes in suspension and primary culture.","volume":"13","author":"Wiebkin","year":"1985","journal-title":"Drug Metab Dispos"},{"key":"10.1124\/dmd.31.8.1035_bib39","series-title":"Detoxification Mechanisms.","author":"Williams","year":"1971"},{"key":"10.1124\/dmd.31.8.1035_bib40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/10408449209145319","article-title":"The human hepatic cytochromes P450 involved in drug metabolism.","volume":"22","author":"Wrighton","year":"1992","journal-title":"Crit Rev Toxicol"},{"key":"10.1124\/dmd.31.8.1035_bib41","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0006-2952(95)02178-7","article-title":"7-Ethoxycoumarin O-deethylation catalyzed by cytochromes P450 1A2 and 2E1 in human liver microsomes.","volume":"51","author":"Yamazaki","year":"1996","journal-title":"Biochem Pharmacol"}],"container-title":["Drug Metabolism and Disposition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0090955624027417?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0090955624027417?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/dmd.31.8.1035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T10:12:02Z","timestamp":1739873522000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0090955624027417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["S0090955624027417"],"URL":"https:\/\/doi.org\/10.1124\/dmd.31.8.1035","relation":{},"ISSN":["0090-9556"],"issn-type":[{"value":"0090-9556","type":"print"}],"subject":[],"published":{"date-parts":[[2003,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"COMPARISON OF PRIMARY HUMAN HEPATOCYTES AND HEPATOMA CELL LINE HEPG2 WITH REGARD TO THEIR BIOTRANSFORMATION PROPERTIES","name":"articletitle","label":"Article Title"},{"value":"Drug Metabolism and Disposition","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/dmd.31.8.1035","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2003 The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}