{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T12:30:23Z","timestamp":1774441823969,"version":"3.50.1"},"reference-count":38,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["jpet.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"published-print":{"date-parts":[[2003,10]]},"DOI":"10.1124\/jpet.103.053033","type":"journal-article","created":{"date-parts":[[2003,9,19]],"date-time":"2003-09-19T00:50:21Z","timestamp":1063932621000},"page":"205-212","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Neuronal Necrosis Inhibition by Insulin through Protein Kinase C Activation"],"prefix":"10.1016","volume":"307","author":[{"given":"Wakako","family":"Hamabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryousuke","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1124\/jpet.103.053033_bib1","doi-asserted-by":"crossref","unstructured":"Bal-Price A and Brown GC (2000) Nitric-oxide-induced necrosis and apoptosis in PC12 cells mediated by mitochondria. J Neurochem 75: 1455\u20131464.","DOI":"10.1046\/j.1471-4159.2000.0751455.x"},{"key":"10.1124\/jpet.103.053033_bib2","doi-asserted-by":"crossref","unstructured":"Barber AJ, Nakamura M, Wolpert EB, Reiter CE, Seigel GM, Antonetti DA, and Gardner TW (2001) Insulin rescues retinal neurons from apoptosis by a phosphatidylinositol 3-kinase\/Akt-mediated mechanism that reduces the activation of caspase-3. J Biol Chem 276: 32814\u201332821.","DOI":"10.1074\/jbc.M104738200"},{"key":"10.1124\/jpet.103.053033_bib3","doi-asserted-by":"crossref","unstructured":"Bose A, Guilherme A, Robida SI, Nicoloro SM, Zhou QL, Jiang ZY, Pomerleau DP, and Czech MP (2002) Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c. Nature (Lond) 420: 821\u2013824.","DOI":"10.1038\/nature01246"},{"key":"10.1124\/jpet.103.053033_bib4","doi-asserted-by":"crossref","unstructured":"Carlson NG, Wieggel WA, Chen J, Bacchi A, Rogers SW, and Gahring LC (1999) Inflammatory cytokines IL-1 alpha, IL-1 beta, IL-6 and TNF-alpha impart neuroprotection to an excitotoxin through distinct pathways. J Immunol 163: 3963\u20133968.","DOI":"10.4049\/jimmunol.163.7.3963"},{"key":"10.1124\/jpet.103.053033_bib5","doi-asserted-by":"crossref","unstructured":"Chibalin AV, Kovalenko MV, Ryder JW, Feraille E, Wallberg-Henriksson H, and Zierath JR (2001) Insulin- and glucose-induced phosphorylation of the Na(+), K(+)-adenosine triphosphatase alpha-subunits in rat skeletal muscle. Endocrinology 142: 3474\u20133482.","DOI":"10.1210\/endo.142.8.8294"},{"key":"10.1124\/jpet.103.053033_bib6","doi-asserted-by":"crossref","unstructured":"Colicos MA and Dash PK (1996) Apoptotic morphology of dentate gyrus granule cells following experimental cortical impact injury in rats: possible role in spatial memory deficits. Brain Res 739: 120\u2013131.","DOI":"10.1016\/S0006-8993(96)00824-4"},{"key":"10.1124\/jpet.103.053033_bib7","doi-asserted-by":"crossref","unstructured":"Dirnagl U, Iadecola C, and Moskowitz MA (1999) Pathobiology of ischaemic stroke: an integrated view. Trends Neurosci 22: 391\u2013397.","DOI":"10.1016\/S0166-2236(99)01401-0"},{"key":"10.1124\/jpet.103.053033_bib8","doi-asserted-by":"crossref","unstructured":"Eichhorn J, Kayali AG, Resor L, Austin DA, Rose DW, and Webster NJ (2002) PLC-gamma1 enzyme activity is required for insulin-induced DNA synthesis. Endocrinology 143: 655\u2013664.","DOI":"10.1210\/endo.143.2.8621"},{"key":"10.1124\/jpet.103.053033_bib9","doi-asserted-by":"crossref","unstructured":"Fox GB, Fan L, Levasseur RA, and Faden AI (1998) Sustained sensory\/motor and cognitive deficits with neuronal apoptosis following controlled cortical impact brain injury in the mouse. J Neurotrauma 15: 599\u2013614.","DOI":"10.1089\/neu.1998.15.599"},{"key":"10.1124\/jpet.103.053033_bib10","doi-asserted-by":"crossref","unstructured":"Friedlander RM, Gagliardini V, Hara H, Fink KB, Li W, MacDonald G, Fishman MC, Greenberg AH, Moskowitz MA, and Yuan J (1997) Expression of a dominant negative mutant of interleukin-1 beta converting enzyme in transgenic mice prevents neuronal cell death induced by trophic factor withdrawal and ischemic brain injury. J Exp Med 185: 933\u2013940.","DOI":"10.1084\/jem.185.5.933"},{"key":"10.1124\/jpet.103.053033_bib11","doi-asserted-by":"crossref","unstructured":"Fujita R and Ueda H (2003) Protein kinase C-mediated necrosis-apoptosis switch of cortical neurons by conditioned medium factors secreted under the serum-free stress. Cell Death Diff 10: 782\u2013790.","DOI":"10.1038\/sj.cdd.4401239"},{"key":"10.1124\/jpet.103.053033_bib12","doi-asserted-by":"crossref","unstructured":"Fujita R, Yoshida A, Mizuno K, and Ueda H (2001) Cell density-dependent death mode switch of cultured cortical neurons under serum-free starvation stress. Cell Mol Neurobiol 21: 317\u2013324.","DOI":"10.1023\/A:1012645920229"},{"key":"10.1124\/jpet.103.053033_bib13","doi-asserted-by":"crossref","unstructured":"Gauthier R, Harnois C, Drolet JF, Reed JC, Vezina A, and Vachon PH (2001) Human intestinal epithelial cell survival: differentiation state-specific control mechanisms. Am J Physiol Cell Physiol 280: C1540\u2013C1554.","DOI":"10.1152\/ajpcell.2001.280.6.C1540"},{"key":"10.1124\/jpet.103.053033_bib14","doi-asserted-by":"crossref","unstructured":"Green DR and Reed JC (1998) Mitochondria and apoptosis. Science (Wash DC) 281: 1309\u20131312.","DOI":"10.1126\/science.281.5381.1309"},{"key":"10.1124\/jpet.103.053033_bib15","doi-asserted-by":"crossref","unstructured":"Ha HC and Snyder SH (1999) Poly (ADP-ribose) polymerase is a mediator of necrotic cell death by ATP depletion. Proc Natl Acad Sci USA 96: 13978\u201313982.","DOI":"10.1073\/pnas.96.24.13978"},{"key":"10.1124\/jpet.103.053033_bib16","doi-asserted-by":"crossref","unstructured":"Halestrap AP, McStay GP, and Clarke SJ (2002) The permeability transition pore complex: another view. Biochimie (Paris) 84: 153\u2013166.","DOI":"10.1016\/S0300-9084(02)01375-5"},{"key":"10.1124\/jpet.103.053033_bib17","doi-asserted-by":"crossref","unstructured":"Hamabe W, Fujita R, Yasusa T, Yoneda F, Yoshida A, and Ueda H (2000) (\u2013)1-(Benzofuran-2-yl)-2-propylaminopentane shows survival effect on cortical neurons under serum-free condition through sigma receptors. Cell Mol Neurobiol 20: 695\u2013702.","DOI":"10.1023\/A:1007050808754"},{"key":"10.1124\/jpet.103.053033_bib18","doi-asserted-by":"crossref","unstructured":"Hara H, Fink K, Endres M, Friedlander RM, Gagliardini V, Yuan J, and Moskowitz MA (1997) Attenuation of transient focal cerebral ischemic injury in transgenic mice expressing a mutant ICE inhibitory protein. J Cereb Blood Flow Metab 17: 370\u2013375.","DOI":"10.1097\/00004647-199704000-00002"},{"key":"10.1124\/jpet.103.053033_bib19","doi-asserted-by":"crossref","unstructured":"Hetman M, Cavanaugh JE, Kimelman D, and Xia Z (2000) Role of glycogen synthase kinase-3beta in neuronal apoptosis induced by trophic withdrawal. J Neurosci 20: 2567\u20132574.","DOI":"10.1523\/JNEUROSCI.20-07-02567.2000"},{"key":"10.1124\/jpet.103.053033_bib20","doi-asserted-by":"crossref","unstructured":"Hetman M, Kanning K, Cavanaugh JE, and Xia Z (1999) Neuroprotection by brain-derived neurotrophic factor is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3-kinase. J Biol Chem 274: 22569\u201322580.","DOI":"10.1074\/jbc.274.32.22569"},{"key":"10.1124\/jpet.103.053033_bib21","doi-asserted-by":"crossref","unstructured":"Huck S (1983) Serum-free medium for cultures of the postnatal mouse cerebellum: only insulin is essential. Brain Res Bull 10: 667\u2013674.","DOI":"10.1016\/0361-9230(83)90036-9"},{"key":"10.1124\/jpet.103.053033_bib22","doi-asserted-by":"crossref","unstructured":"Johnson SA and Denton RM (2003) Insulin stimulation of pyruvate dehydrogenase in adipocytes involves two distinct signalling pathways. Biochem J 369: 351\u2013356.","DOI":"10.1042\/bj20020920"},{"key":"10.1124\/jpet.103.053033_bib23","doi-asserted-by":"crossref","unstructured":"Koivisto UM, Martinez-Valdez H, Bilan PJ, Burdett E, Ramlal T, and Klip A (1991) Differential regulation of the GLUT-1 and GLUT-4 glucose transport systems by glucose and insulin in L6 muscle cells in culture. J Biol Chem 266: 2615\u20132621.","DOI":"10.1016\/S0021-9258(18)52289-4"},{"key":"10.1124\/jpet.103.053033_bib24","doi-asserted-by":"crossref","unstructured":"Lorenzo M, Teruel T, Hernandez R, Kayali AG, and Webster NJ (2002) PLCgamma participates in insulin stimulation of glucose uptake through activation of PKC-zeta in brown adipocytes. Exp Cell Res 278: 146\u2013157.","DOI":"10.1006\/excr.2002.5570"},{"key":"10.1124\/jpet.103.053033_bib25","unstructured":"Malis CD and Bonventre JV (1988) Susceptibility of mitochondrial membranes to calcium and reactive oxygen species: implications for ischemic and toxic tissue damage. Prog Clin Biol Res 282: 235\u2013259."},{"key":"10.1124\/jpet.103.053033_bib26","unstructured":"Mosinger Ogilvie J, Deckwerth TL, Knudson CM, and Korsmeyer SJ (1998) Suppression of developmental retinal cell death but not of photoreceptor degeneration in Bax-deficient mice. Investig Ophthalmol Vis Sci 39: 1713\u20131720."},{"key":"10.1124\/jpet.103.053033_bib27","doi-asserted-by":"crossref","unstructured":"Pessin JE and Saltiel AR (2000) Signaling pathways in insulin action: molecular targets of insulin resistance. J Clin Investig 106: 165\u2013169.","DOI":"10.1172\/JCI10582"},{"key":"10.1124\/jpet.103.053033_bib28","doi-asserted-by":"crossref","unstructured":"Raff M (1998) Cell suicide for beginners. Nature (Lond) 396: 119\u2013122.","DOI":"10.1038\/24055"},{"key":"10.1124\/jpet.103.053033_bib29","doi-asserted-by":"crossref","unstructured":"Ryu BR, Ko HW, Jou I, Noh JS, and Gwag BJ (1999) Phosphatidylinositol 3-kinase-mediated regulation of neuronal apoptosis and necrosis by insulin and IGF-I. J Neurobiol 39: 536\u2013546.","DOI":"10.1002\/(SICI)1097-4695(19990615)39:4<536::AID-NEU7>3.0.CO;2-J"},{"key":"10.1124\/jpet.103.053033_bib30","doi-asserted-by":"crossref","unstructured":"Salamanca MC and Mathers DA (1987) Mouse spinal cord neurons in serum-free culture media: suitability for patch clamp studies on chemical and electrical excitability. Dev Neurosci 9: 174\u2013182.","DOI":"10.1159\/000111620"},{"key":"10.1124\/jpet.103.053033_bib31","doi-asserted-by":"crossref","unstructured":"Saltiel AR (2001) New perspectives into the molecular pathogenesis and treatment of type 2 diabetes. Cell 104: 517\u2013529.","DOI":"10.1016\/S0092-8674(01)00239-2"},{"key":"10.1124\/jpet.103.053033_bib32","doi-asserted-by":"crossref","unstructured":"Sasaki Y, Fukushima N, Yoshida A, and Ueda H (1998) Low-density induced apoptosis of cortical neurons is inhibited by serum factors. Cell Mol Neurobiol 18: 487\u2013496.","DOI":"10.1023\/A:1026375225275"},{"key":"10.1124\/jpet.103.053033_bib33","doi-asserted-by":"crossref","unstructured":"Skaper SD, Selak I, Manthorpe M, and Varon S (1984) Chemically defined requirements for the survival of cultured 8-day chick embryo ciliary ganglion neurons. Brain Res 302: 281\u2013290.","DOI":"10.1016\/0006-8993(84)90241-5"},{"key":"10.1124\/jpet.103.053033_bib34","doi-asserted-by":"crossref","unstructured":"Slaaby R, Du G, Altshuller YM, Frohman MA, and Seedorf K (2000) Insulin-induced phospholipase D1 and phospholipase D2 activity in human embryonic kidney-293 cells mediated by the phospholipase C gamma and protein kinase C alpha signalling cascade. Biochem J 351: 613\u2013619.","DOI":"10.1042\/bj3510613"},{"key":"10.1124\/jpet.103.053033_bib35","doi-asserted-by":"crossref","unstructured":"Snyder EY and Kim SU (1980) Insulin: is it a nerve survival factor? Brain Res 196: 565\u2013571.","DOI":"10.1016\/0006-8993(80)90426-6"},{"key":"10.1124\/jpet.103.053033_bib36","doi-asserted-by":"crossref","unstructured":"Sweeney G, Niu W, Canfield VA, Levenson R, and Klip A (2001) Insulin increases plasma membrane content and reduces phosphorylation of Na+-K+ pump \u03b11-subunit in HEK-293 cells. Am J Physiol Cell Physiol 281: C1797\u2013C1803.","DOI":"10.1152\/ajpcell.2001.281.6.C1797"},{"key":"10.1124\/jpet.103.053033_bib37","doi-asserted-by":"crossref","unstructured":"Watson RT and Pessin JE (2001) Intracellular organization of insulin signaling and GLUT4 translocation. Recent Prog Horm Res 56: 175\u2013193.","DOI":"10.1210\/rp.56.1.175"},{"key":"10.1124\/jpet.103.053033_bib38","doi-asserted-by":"crossref","unstructured":"Xiao AY, Wei L, Xia S, Rothman S, and Yu SP (2002) Ionic mechanism of ouabain-induced concurrent apoptosis and necrosis in individual cultured cortical neurons. J Neurosci 22: 1350\u20131362.","DOI":"10.1523\/JNEUROSCI.22-04-01350.2002"}],"container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524309462?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524309462?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/jpet.103.053033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T14:32:55Z","timestamp":1739975575000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022356524309462"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,10]]}},"alternative-id":["S0022356524309462"],"URL":"https:\/\/doi.org\/10.1124\/jpet.103.053033","relation":{},"ISSN":["0022-3565"],"issn-type":[{"value":"0022-3565","type":"print"}],"subject":[],"published":{"date-parts":[[2003,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Neuronal Necrosis Inhibition by Insulin through Protein Kinase C Activation","name":"articletitle","label":"Article Title"},{"value":"The Journal of Pharmacology and Experimental Therapeutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/jpet.103.053033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2003 The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}