{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:17:57Z","timestamp":1780510677248,"version":"3.54.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["jpet.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"published-print":{"date-parts":[[2008,6]]},"DOI":"10.1124\/jpet.107.132944","type":"journal-article","created":{"date-parts":[[2008,3,6]],"date-time":"2008-03-06T02:40:28Z","timestamp":1204771228000},"page":"824-832","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":33,"title":["Differential Effect of the Protein Synthesis Inhibitors Puromycin and Cycloheximide on Vascular Smooth Muscle Cell Viability"],"prefix":"10.1016","volume":"325","author":[{"given":"Valerie","family":"Croons","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Martinet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold G.","family":"Herman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido R.Y.","family":"De Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/jpet.107.132944_bib1","doi-asserted-by":"crossref","unstructured":"Azzam ME and Algranati ID (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent protein chains from polysomes. Proc Natl Acad Sci U S A 70: 3866-3869.","DOI":"10.1073\/pnas.70.12.3866"},{"key":"10.1124\/jpet.107.132944_bib2","doi-asserted-by":"crossref","unstructured":"Booth RF, Martin JF, Honey AC, Hassall DG, Beesley JE, and Moncada S (1989) Rapid development of atherosclerotic lesions in the rabbit carotid artery induced by perivascular manipulation. Atherosclerosis 76: 257-268.","DOI":"10.1016\/0021-9150(89)90109-3"},{"key":"10.1124\/jpet.107.132944_bib3","doi-asserted-by":"crossref","unstructured":"Boyle JJ, Bowyer DE, Weissberg PL, and Bennett MR (2001) Human blood-derived macrophages induce apoptosis in human plaque-derived vascular smooth muscle cells by Fas-ligand\/Fas interactions. Arterioscler Thromb Vasc Biol 21: 1402-1407.","DOI":"10.1161\/hq0901.094279"},{"key":"10.1124\/jpet.107.132944_bib4","doi-asserted-by":"crossref","unstructured":"Croons V, Martinet W, Herman AG, Timmermans JP, and De Meyer GRY (2007) Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide. J Pharmacol Exp Ther 320: 986-993.","DOI":"10.1124\/jpet.106.113944"},{"key":"10.1124\/jpet.107.132944_bib5","doi-asserted-by":"crossref","unstructured":"De Meyer GRY, Kockx MM, Cromheeke KM, Seye CI, Herman AG, and Bult H (2000) Periadventitial inducible nitric oxide synthase expression and intimal thickening. Arterioscler Thromb Vasc Biol 20: 1896-1902.","DOI":"10.1161\/01.ATV.20.8.1896"},{"key":"10.1124\/jpet.107.132944_bib6","doi-asserted-by":"crossref","unstructured":"De Meyer GRY, Kockx MM, Knaapen MW, Martinet W, De Cleen DM, Bult H, and Herman AG (2003) Nitric oxide donor molsidomine favors features of atherosclerotic plaque stability during cholesterol lowering in rabbits. J Cardiovasc Pharmacol 41: 970-978.","DOI":"10.1097\/00005344-200306000-00021"},{"key":"10.1124\/jpet.107.132944_bib7","doi-asserted-by":"crossref","unstructured":"De Meyer GRY, Van Put DJ, Kockx MM, Van Schil P, Bosmans R, Bult H, Buyssens N, Vanmaele R, and Herman AG (1997) Possible mechanisms of collar-induced intimal thickening. Arterioscler Thromb Vasc Biol 17: 1924-1930.","DOI":"10.1161\/01.ATV.17.10.1924"},{"key":"10.1124\/jpet.107.132944_bib8","doi-asserted-by":"crossref","unstructured":"Galis ZS, Sukhova GK, Lark MW, and Libby P (1994) Increased expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques. J Clin Invest 94: 2493-2503.","DOI":"10.1172\/JCI117619"},{"key":"10.1124\/jpet.107.132944_bib9","doi-asserted-by":"crossref","unstructured":"Garcia-Mata R, Gao YS, and Sztul E (2002) Hassles with taking out the garbage: aggravating aggresomes. Traffic 3: 388-396.","DOI":"10.1034\/j.1600-0854.2002.30602.x"},{"key":"10.1124\/jpet.107.132944_bib10","doi-asserted-by":"crossref","unstructured":"Hashimoto M, Rockenstein E, Crews L, and Masliah E (2003) Role of protein aggregation in mitochondrial dysfunction and neurodegeneration in Alzheimer\u2019s and Parkinson\u2019s diseases. Neuromolecular Med 4: 21-36.","DOI":"10.1385\/NMM:4:1-2:21"},{"key":"10.1124\/jpet.107.132944_bib11","doi-asserted-by":"crossref","unstructured":"Ito K, Kiyosawa N, Kumagai K, Manabe S, Matsunuma N, and Yamoto T (2006) Molecular mechanism investigation of cycloheximide-induced hepatocyte apoptosis in rat livers by morphological and microarray analysis. Toxicology 219: 175-186.","DOI":"10.1016\/j.tox.2005.11.017"},{"key":"10.1124\/jpet.107.132944_bib12","doi-asserted-by":"crossref","unstructured":"Kockx MM, De Meyer GRY, Jacob WA, Bult H, and Herman AG (1992) Triphasic sequence of neointimal formation in the cuffed carotid artery of the rabbit. Arterioscler Thromb 12: 1447-1457.","DOI":"10.1161\/01.ATV.12.12.1447"},{"key":"10.1124\/jpet.107.132944_bib13","doi-asserted-by":"crossref","unstructured":"Koenig W and Khuseyinova N (2007) Biomarkers of atherosclerotic plaque instability and rupture. Arterioscler Thromb Vasc Biol 27: 15-26.","DOI":"10.1161\/01.ATV.0000251503.35795.4f"},{"key":"10.1124\/jpet.107.132944_bib14","doi-asserted-by":"crossref","unstructured":"Kumagai K, Ando Y, Kiyosawa N, Ito K, Kawai R, Yamoto T, Manabe S, and Teranishi M (2006) Toxicoproteomic investigation of the molecular mechanisms of cycloheximide-induced hepatocellular apoptosis in rat liver. Toxicology 228: 299-309.","DOI":"10.1016\/j.tox.2006.09.015"},{"key":"10.1124\/jpet.107.132944_bib15","doi-asserted-by":"crossref","unstructured":"Lafont A (2003) Basic aspects of plaque vulnerability. Heart 89: 1262-1267.","DOI":"10.1136\/heart.89.10.1262"},{"key":"10.1124\/jpet.107.132944_bib16","doi-asserted-by":"crossref","unstructured":"Lamkanfi M, Kalai M, and Vandenabeele P (2004) Caspase-12: an overview. Cell Death Differ 11: 365-368.","DOI":"10.1038\/sj.cdd.4401364"},{"key":"10.1124\/jpet.107.132944_bib17","doi-asserted-by":"crossref","unstructured":"Lelouard H, Ferrand V, Marguet D, Bania J, Camosseto V, David A, Gatti E, and Pierre P (2004) Dendritic cell aggresome-like induced structures are dedicated areas for ubiquitination and storage of newly synthesized defective proteins. J Cell Biol 164: 667-675.","DOI":"10.1083\/jcb.200312073"},{"key":"10.1124\/jpet.107.132944_bib18","doi-asserted-by":"crossref","unstructured":"Libby P and Theroux P (2005) Pathophysiology of coronary artery disease. Circulation 111: 3481-3488.","DOI":"10.1161\/CIRCULATIONAHA.105.537878"},{"key":"10.1124\/jpet.107.132944_bib19","doi-asserted-by":"crossref","unstructured":"L\u00f6wik CW, Alblas MJ, van de RM, Papapoulos SE, and van der Pluijm G (1993) Quantification of adherent and nonadherent cells cultured in 96-well plates using the supravital stain neutral red. Anal Biochem 213: 426-433.","DOI":"10.1006\/abio.1993.1442"},{"key":"10.1124\/jpet.107.132944_bib20","doi-asserted-by":"crossref","unstructured":"Lutgens E, van Suylen RJ, Faber BC, Gijbels MJ, Eurlings PM, Bijnens AP, Cleutjens KB, Heeneman S, and Daemen MJ (2003) Atherosclerotic plaque rupture: local or systemic process? Arterioscler Thromb Vasc Biol 23: 2123-2130.","DOI":"10.1161\/01.ATV.0000097783.01596.E2"},{"key":"10.1124\/jpet.107.132944_bib21","doi-asserted-by":"crossref","unstructured":"Martinet W, Croons V, Timmermans JP, Herman AG, and De Meyer GRY (2007a) Nitric oxide selectively depletes macrophages in atherosclerotic plaques via induction of endoplasmic reticulum stress. Br J Pharmacol 152: 493-500.","DOI":"10.1038\/sj.bjp.0707426"},{"key":"10.1124\/jpet.107.132944_bib22","doi-asserted-by":"crossref","unstructured":"Martinet W and De Meyer GRY (2007) Selective depletion of macrophages in atherosclerotic plaques: myth, hype, or reality? Circ Res 100: 751-753.","DOI":"10.1161\/01.RES.0000263397.14481.96"},{"key":"10.1124\/jpet.107.132944_bib23","doi-asserted-by":"crossref","unstructured":"Martinet W and Kockx MM (2001) Apoptosis in atherosclerosis: focus on oxidized lipids and inflammation. Curr Opin Lipidol 12: 535-541.","DOI":"10.1097\/00041433-200110000-00009"},{"key":"10.1124\/jpet.107.132944_bib24","doi-asserted-by":"crossref","unstructured":"Martinet W, Verheye S, and De Meyer GRY (2007b) Selective depletion of macrophages in atherosclerotic plaques via macrophage-specific initiation of cell death. Trends Cardiovasc Med 17: 69-75.","DOI":"10.1016\/j.tcm.2006.12.004"},{"key":"10.1124\/jpet.107.132944_bib25","doi-asserted-by":"crossref","unstructured":"McCullough KD, Martindale JL, Klotz LO, Aw TY, and Holbrook NJ (2001) Gadd153 sensitizes cells to endoplasmic reticulum stress by down-regulating Bcl2 and perturbing the cellular redox state. Mol Cell Biol 21: 1249-1259.","DOI":"10.1128\/MCB.21.4.1249-1259.2001"},{"key":"10.1124\/jpet.107.132944_bib26","doi-asserted-by":"crossref","unstructured":"Momoi T (2004) Caspases involved in ER stress-mediated cell death. J Chem Neuroanat 28: 101-105.","DOI":"10.1016\/j.jchemneu.2004.05.008"},{"key":"10.1124\/jpet.107.132944_bib27","doi-asserted-by":"crossref","unstructured":"Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, and Yuan J (2000) Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 403: 98-103.","DOI":"10.1038\/47513"},{"key":"10.1124\/jpet.107.132944_bib28","doi-asserted-by":"crossref","unstructured":"Newby AC (2005) Dual role of matrix metalloproteinases (matrixins) in intimal thickening and atherosclerotic plaque rupture. Physiol Rev 85: 1-31.","DOI":"10.1152\/physrev.00048.2003"},{"key":"10.1124\/jpet.107.132944_bib29","doi-asserted-by":"crossref","unstructured":"Okada K, Minamino T, Tsukamoto Y, Liao Y, Tsukamoto O, Takashima S, Hirata A, Fujita M, Nagamachi Y, Nakatani T, et al. (2004) Prolonged endoplasmic reticulum stress in hypertrophic and failing heart after aortic constriction: possible contribution of endoplasmic reticulum stress to cardiac myocyte apoptosis. Circulation 110: 705-712.","DOI":"10.1161\/01.CIR.0000137836.95625.D4"},{"key":"10.1124\/jpet.107.132944_bib30","doi-asserted-by":"crossref","unstructured":"Patterson C, Ike C, Willis PW, Stouffer GA, and Willis MS (2007) The bitter end: the ubiquitin-proteasome system and cardiac dysfunction. Circulation 115: 1456-1463.","DOI":"10.1161\/CIRCULATIONAHA.106.649863"},{"key":"10.1124\/jpet.107.132944_bib31","doi-asserted-by":"crossref","unstructured":"Qi X, Hosoi T, Okuma Y, Kaneko M, and Nomura Y (2004) Sodium 4-phenylbutyrate protects against cerebral ischemic injury. Mol Pharmacol 66: 899-908.","DOI":"10.1124\/mol.104.001339"},{"key":"10.1124\/jpet.107.132944_bib32","doi-asserted-by":"crossref","unstructured":"Raught B, Gingras AC, and Sonenberg N (2001) The target of rapamycin (TOR) proteins. Proc Natl Acad Sci U S A 98: 7037-7044.","DOI":"10.1073\/pnas.121145898"},{"key":"10.1124\/jpet.107.132944_bib33","doi-asserted-by":"crossref","unstructured":"Rutkowski DT and Kaufman RJ (2004) A trip to the ER: coping with stress. Trends Cell Biol 14: 20-28.","DOI":"10.1016\/j.tcb.2003.11.001"},{"key":"10.1124\/jpet.107.132944_bib34","doi-asserted-by":"crossref","unstructured":"Schrijvers DM, De Meyer GRY, Kockx M, Herman AG, and Martinet W (2006) Comparison of apoptosis detection markers combined with macrophage immunostaining to study phagocytosis of apoptotic cells in situ. Biomark Insights 2: 193-200.","DOI":"10.1177\/117727190600100018"},{"key":"10.1124\/jpet.107.132944_bib35","doi-asserted-by":"crossref","unstructured":"Shah PK (2003) Mechanisms of plaque vulnerability and rupture. J Am Coll Cardiol 41: 15S-22S.","DOI":"10.1016\/S0735-1097(02)02834-6"},{"key":"10.1124\/jpet.107.132944_bib36","doi-asserted-by":"crossref","unstructured":"Shen X, Zhang K, and Kaufman RJ (2004) The unfolded protein response\u2013a stress signaling pathway of the endoplasmic reticulum. J Chem Neuroanat 28: 79-92.","DOI":"10.1016\/j.jchemneu.2004.02.006"},{"key":"10.1124\/jpet.107.132944_bib37","doi-asserted-by":"crossref","unstructured":"Sidhu JS and Omiecinski CJ (1998) Protein synthesis inhibitors exhibit a nonspecific effect on phenobarbital-inducible cytochrome P450 gene expression in primary rat hepatocytes. J Biol Chem 273: 4769-4775.","DOI":"10.1074\/jbc.273.8.4769"},{"key":"10.1124\/jpet.107.132944_bib38","doi-asserted-by":"crossref","unstructured":"St\u00f6ckleinW and Piepersberg W (1980) Binding of cycloheximide to ribosomes from wild-type and mutant strains of Saccharomyces cerevisiae. Antimicrob Agents Chemother 18: 863-867.","DOI":"10.1128\/AAC.18.6.863"},{"key":"10.1124\/jpet.107.132944_bib39","doi-asserted-by":"crossref","unstructured":"Verheye S, Martinet W, Kockx MM, Knaapen MW, Salu K, Timmermans JP, Ellis JT, Kilpatrick DL, and De Meyer GRY (2007) Selective clearance of macrophages in atherosclerotic plaques by autophagy. J Am Coll Cardiol 49: 706-715.","DOI":"10.1016\/j.jacc.2006.09.047"},{"key":"10.1124\/jpet.107.132944_bib40","doi-asserted-by":"crossref","unstructured":"Xu C, Bailly-Maitre B, and Reed JC (2005) Endoplasmic reticulum stress: cell life and death decisions. J Clin Invest 115: 2656-2664.","DOI":"10.1172\/JCI26373"}],"container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524344544?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524344544?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/jpet.107.132944","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T22:38:31Z","timestamp":1740004711000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022356524344544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["S0022356524344544"],"URL":"https:\/\/doi.org\/10.1124\/jpet.107.132944","relation":{},"ISSN":["0022-3565"],"issn-type":[{"value":"0022-3565","type":"print"}],"subject":[],"published":{"date-parts":[[2008,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Differential Effect of the Protein Synthesis Inhibitors Puromycin and Cycloheximide on Vascular Smooth Muscle Cell Viability","name":"articletitle","label":"Article Title"},{"value":"The Journal of Pharmacology and Experimental Therapeutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/jpet.107.132944","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2008 The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}