{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:29:18Z","timestamp":1792718958924,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["jpet.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"published-print":{"date-parts":[[2008,9]]},"DOI":"10.1124\/jpet.107.135798","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T01:04:22Z","timestamp":1213319062000},"page":"829-837","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"title":["The Protein Kinase A Pathway Contributes to Hg2+-Induced Alterations in Phosphorylation and Subcellular Distribution of Occludin Associated with Increased Tight Junction Permeability of Salivary Epithelial Cell Monolayers"],"prefix":"10.1016","volume":"326","author":[{"given":"Jitesh D.","family":"Kawedia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Kulkarni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly E.","family":"Waechter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl S.","family":"Matlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni M.","family":"Pauletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil G.","family":"Menon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/jpet.107.135798_bib1","doi-asserted-by":"crossref","unstructured":"Adson A, Raub TJ, Burton PS, Barsuhn CL, Hilgers AR, Audus KL, and Ho NF (1994) Quantitative approaches to delineate paracellular diffusion in cultured epithelial cell monolayers. J Pharm Sci 83: 1529-1536.","DOI":"10.1002\/jps.2600831103"},{"key":"10.1124\/jpet.107.135798_bib2","doi-asserted-by":"crossref","unstructured":"B\u00f6hme M, Diener M, Mestres P, and Rummel W (1992) Direct and indirect actions of HgCl2 and methyl mercury chloride on permeability and chloride secretion across the rat colonic mucosa. Toxicol Appl Pharmacol 114: 285-294.","DOI":"10.1016\/0041-008X(92)90079-8"},{"key":"10.1124\/jpet.107.135798_bib3","doi-asserted-by":"crossref","unstructured":"Burghardt B, Nielsen S, and Steward MC (2006) The role of aquaporin water channels in fluid secretion by the exocrine pancreas. J Membr Biol 210: 143-153.","DOI":"10.1007\/s00232-005-0852-6"},{"key":"10.1124\/jpet.107.135798_bib4","doi-asserted-by":"crossref","unstructured":"Cordenonsi M, Mazzon E, De Rigo L, Baraldo S, Meggio F, and Citi S (1997) Occludin dephosphorylation in early development of Xenopus laevis. J Cell Sci 110: 3131-3139.","DOI":"10.1242\/jcs.110.24.3131"},{"key":"10.1124\/jpet.107.135798_bib5","doi-asserted-by":"crossref","unstructured":"El-Sayed S and Nelson N (1996) Adjuvant and adjunctive chemotherapy in the management of squamous cell carcinoma of the head and neck region. A meta-analysis of prospective and randomized trials. J Clin Oncol 14: 838-847.","DOI":"10.1200\/JCO.1996.14.3.838"},{"key":"10.1124\/jpet.107.135798_bib6","doi-asserted-by":"crossref","unstructured":"Feldman GJ, Mullin JM, and Ryan MP (2005) Occludin: structure, function and regulation. Adv Drug Deliv Rev 57: 883-917.","DOI":"10.1016\/j.addr.2005.01.009"},{"key":"10.1124\/jpet.107.135798_bib7","doi-asserted-by":"crossref","unstructured":"Fey EG, Wan KM, and Penman S (1984) Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition. J Cell Biol 98: 1973-1984.","DOI":"10.1083\/jcb.98.6.1973"},{"key":"10.1124\/jpet.107.135798_bib8","doi-asserted-by":"crossref","unstructured":"Folkesson HG, Matthay MA, Hasegawa H, Kheradmand F, and Verkman AS (1994) Transcellular water transport in lung alveolar epithelium through mercury-sensitive water channels. Proc Natl Acad Sci U S A 91: 4970-4974.","DOI":"10.1073\/pnas.91.11.4970"},{"key":"10.1124\/jpet.107.135798_bib9","doi-asserted-by":"crossref","unstructured":"Harhaj NS, Felinski EA, Wolpert EB, Sundstrom JM, Gardner TW, and Antonetti DA (2006) VEGF activation of protein kinase C stimulates occludin phosphorylation and contributes to endothelial permeability. Invest Ophthalmol Vis Sci 47: 5106-5115.","DOI":"10.1167\/iovs.06-0322"},{"key":"10.1124\/jpet.107.135798_bib10","doi-asserted-by":"crossref","unstructured":"Hecht G, Pothoulakis C, LaMont JT, and Madara JL (1988) Clostridium difficile toxin A perturbs cytoskeletal structure and tight junction permeability of cultured human intestinal epithelial monolayers. J Clin Invest 82: 1516-1524.","DOI":"10.1172\/JCI113760"},{"key":"10.1124\/jpet.107.135798_bib11","doi-asserted-by":"crossref","unstructured":"Hirase T, Kawashima S, Wong EY, Ueyama T, Rikitake Y, Tsukita S, Yokoyama M, and Staddon JM (2001) Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms. J Biol Chem 276: 10423-10431.","DOI":"10.1074\/jbc.M007136200"},{"key":"10.1124\/jpet.107.135798_bib12","doi-asserted-by":"crossref","unstructured":"Kawedia JD, Nieman ML, Boivin GP, Melvin JE, Kikuchi K, Hand AR, Lorenz JN, and Menon AG (2007) Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex. Proc Natl Acad Sci U S A 104: 3621-3626.","DOI":"10.1073\/pnas.0608384104"},{"key":"10.1124\/jpet.107.135798_bib13","doi-asserted-by":"crossref","unstructured":"Kevil CG, Oshima T, Alexander B, Coe LL, and Alexander JS (2000) H(2)O(2)-mediated permeability: role of MAPK and occludin. Am J Physiol Cell Physiol 279: C21-C30.","DOI":"10.1152\/ajpcell.2000.279.1.C21"},{"key":"10.1124\/jpet.107.135798_bib14","doi-asserted-by":"crossref","unstructured":"Klingler C, Kniesel U, Bamforth SD, Wolburg H, Engelhardt B, and Risau W (2000) Disruption of epithelial tight junctions is prevented by cyclic nucleotide-dependent protein kinase inhibitors. Histochem Cell Biol 113: 349-361.","DOI":"10.1007\/s004180000143"},{"key":"10.1124\/jpet.107.135798_bib15","doi-asserted-by":"crossref","unstructured":"Ko SB, Naruse S, Kitagawa M, Ishiguro H, Furuya S, Mizuno N, Wang Y, Yoshikawa T, Suzuki A, Shimano S, et al. (2002) Aquaporins in rat pancreatic interlobular ducts. Am J Physiol Gastrointest Liver Physiol 282: G324-G331.","DOI":"10.1152\/ajpgi.00198.2001"},{"key":"10.1124\/jpet.107.135798_bib16","doi-asserted-by":"crossref","unstructured":"Lindmark T, Nikkil\u00e4 T, and Artursson P (1995) Mechanisms of absorption enhancement by medium chain fatty acids in intestinal epithelial Caco-2 cell monolayers. J Pharmacol Exp Ther 275: 958-964.","DOI":"10.1016\/S0022-3565(25)12150-2"},{"key":"10.1124\/jpet.107.135798_bib17","doi-asserted-by":"crossref","unstructured":"Ma TY, Hoa NT, Tran DD, Bui V, Pedram A, Mills S, and Merryfield M (2000) Cytochalasin B modulation of Caco-2 tight junction barrier: role of myosin light chain kinase. Am J Physiol Gastrointest Liver Physiol 279: G875-G885.","DOI":"10.1152\/ajpgi.2000.279.5.G875"},{"key":"10.1124\/jpet.107.135798_bib18","doi-asserted-by":"crossref","unstructured":"Macey RI (1984) Transport of water and urea in red blood cells. Am J Physiol Cell Physiol 246: C195-C203.","DOI":"10.1152\/ajpcell.1984.246.3.C195"},{"key":"10.1124\/jpet.107.135798_bib19","doi-asserted-by":"crossref","unstructured":"Pauletti GM, Gangwar S, Okumu FW, Siahaan TJ, Stella VJ, and Borchardt RT (1996) Esterase-sensitive cyclic prodrugs of peptides: evaluation of an acyloxy-alkoxy promoiety in a model hexapeptide. Pharm Res 13: 1615-1623.","DOI":"10.1023\/A:1016472119387"},{"key":"10.1124\/jpet.107.135798_bib20","doi-asserted-by":"crossref","unstructured":"Preston GM, Jung JS, Guggino WB, and Agre P (1993) The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel. J Biol Chem 268: 17-20.","DOI":"10.1016\/S0021-9258(18)54108-9"},{"key":"10.1124\/jpet.107.135798_bib21","doi-asserted-by":"crossref","unstructured":"Prichard MN, Daily SL, Jefferson GM, Perry AL, and Kern ER (2007) A rapid DNA hybridization assay for the evaluation of antiviral compounds against Epstein-Barr virus. J Virol Methods 144: 86-90.","DOI":"10.1016\/j.jviromet.2007.04.001"},{"key":"10.1124\/jpet.107.135798_bib22","doi-asserted-by":"crossref","unstructured":"Quissell DO, Barzen KA, Gruenert DC, Redman RS, Camden JM, and Turner JT (1997) Development and characterization of SV40 immortalized rat submandibular acinar cell lines. In Vitro Cell Dev Biol Anim 33: 164-173.","DOI":"10.1007\/s11626-997-0137-8"},{"key":"10.1124\/jpet.107.135798_bib23","doi-asserted-by":"crossref","unstructured":"Rajasekaran SA, Barwe SP, Gopal J, Ryazantsev S, Schneeberger EE, and Rajasekaran AK (2007) Na-K-ATPase regulates tight junction permeability through occludin phosphorylation in pancreatic epithelial cells. Am J Physiol Gastrointest Liver Physiol 292: G124-G133.","DOI":"10.1152\/ajpgi.00297.2006"},{"key":"10.1124\/jpet.107.135798_bib24","doi-asserted-by":"crossref","unstructured":"Rao RK, Basuroy S, Rao VU, Karnaky KJ Jr, and Gupta A (2002) Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-beta-catenin complexes from the cytoskeleton by oxidative stress. Biochem J 368: 471-481.","DOI":"10.1042\/bj20011804"},{"key":"10.1124\/jpet.107.135798_bib25","unstructured":"Renkin EM (1954) Filtration, diffusion, and molecular sieving through porous cellulose membranes. J Gen Physiol 38: 225-243."},{"key":"10.1124\/jpet.107.135798_bib26","doi-asserted-by":"crossref","unstructured":"Roberts SK, Yano M, Ueno Y, Pham L, Alpini G, Agre P, and LaRusso NF (1994) Cholangiocytes express the aquaporin CHIP and transport water via a channel-mediated mechanism. Proc Natl Acad Sci U S A 91: 13009-13013.","DOI":"10.1073\/pnas.91.26.13009"},{"key":"10.1124\/jpet.107.135798_bib27","doi-asserted-by":"crossref","unstructured":"Sakakibara A, Furuse M, Saitou M, Ando-Akatsuka Y, and Tsukita S (1997) Possible involvement of phosphorylation of occludin in tight junction formation. J Cell Biol 137: 1393-1401.","DOI":"10.1083\/jcb.137.6.1393"},{"key":"10.1124\/jpet.107.135798_bib28","doi-asserted-by":"crossref","unstructured":"Savage DF and Stroud RM (2007) Structural basis of aquaporin inhibition by mercury. J Mol Biol 368: 607-617.","DOI":"10.1016\/j.jmb.2007.02.070"},{"key":"10.1124\/jpet.107.135798_bib29","doi-asserted-by":"crossref","unstructured":"Schnitzer JE and Oh P (1996) Aquaporin-1 in plasma membrane and caveolae provides mercury-sensitive water channels across lung endothelium. Am J Physiol Heart Circ Physiol 270: H416-H422.","DOI":"10.1152\/ajpheart.1996.270.1.H416"},{"key":"10.1124\/jpet.107.135798_bib30","doi-asserted-by":"crossref","unstructured":"Shen L, Black ED, Witkowski ED, Lencer WI, Guerriero V, Schneeberger EE, and Turner JR (2006) Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure. J Cell Sci 119: 2095-2106.","DOI":"10.1242\/jcs.02915"},{"key":"10.1124\/jpet.107.135798_bib31","doi-asserted-by":"crossref","unstructured":"Stuart RO, Sun A, Bush KT, and Nigam SK (1996) Dependence of epithelial intercellular junction biogenesis on thapsigargin-sensitive intracellular calcium stores. J Biol Chem 271: 13636-13641.","DOI":"10.1074\/jbc.271.23.13636"},{"key":"10.1124\/jpet.107.135798_bib32","doi-asserted-by":"crossref","unstructured":"Stuart RO, Sun A, Panichas M, Hebert SC, Brenner BM, and Nigam SK (1994) Critical role for intracellular calcium in tight junction biogenesis. J Cell Physiol 159: 423-433.","DOI":"10.1002\/jcp.1041590306"},{"key":"10.1124\/jpet.107.135798_bib33","doi-asserted-by":"crossref","unstructured":"van der Reijden WA, Vissink A, Veerman EC, and Amerongen AV (1999) Treatment of oral dryness related complaints (xerostomia) in Sj\u00f6gren\u2019s syndrome. Ann Rheum Dis 58: 465-474.","DOI":"10.1136\/ard.58.8.465"},{"key":"10.1124\/jpet.107.135798_bib34","doi-asserted-by":"crossref","unstructured":"Wachtel M, Frei K, Ehler E, Fontana A, Winterhalter K, and Gloor SM (1999) Occludin proteolysis and increased permeability in endothelial cells through tyrosine phosphatase inhibition. J Cell Sci 112: 4347-4356.","DOI":"10.1242\/jcs.112.23.4347"},{"key":"10.1124\/jpet.107.135798_bib35","doi-asserted-by":"crossref","unstructured":"Wang Q, Luo W, Zheng W, Liu Y, Xu H, Zheng G, Dai Z, Zhang W, Chen Y, and Chen J (2007) Iron supplement prevents lead-induced disruption of the blood-brain barrier during rat development. Toxicol Appl Pharmacol 219: 33-41.","DOI":"10.1016\/j.taap.2006.11.035"},{"key":"10.1124\/jpet.107.135798_bib36","doi-asserted-by":"crossref","unstructured":"Whittembury G (1962) Action of antidiuretic hormone on the equivalent pore radius at both surfaces of the epithelium of the isolated toad skin. J Gen Physiol 46: 117-130.","DOI":"10.1085\/jgp.46.1.117"},{"key":"10.1124\/jpet.107.135798_bib37","doi-asserted-by":"crossref","unstructured":"Wong V (1997) Phosphorylation of occludin correlates with occludin localization and function at the tight junction. Am J Physiol Cell Physiol 273: C1859-C1867.","DOI":"10.1152\/ajpcell.1997.273.6.C1859"},{"key":"10.1124\/jpet.107.135798_bib38","doi-asserted-by":"crossref","unstructured":"Wong V and Gumbiner BM (1997) A synthetic peptide corresponding to the extracellular domain of occludin perturbs the tight junction permeability barrier. J Cell Biol 136: 399-409.","DOI":"10.1083\/jcb.136.2.399"},{"key":"10.1124\/jpet.107.135798_bib39","doi-asserted-by":"crossref","unstructured":"Yang B, Kim JK, and Verkman AS (2006) Comparative efficacy of HgCl2 with candidate aquaporin-1 inhibitors DMSO, gold, TEA+ and acetazolamide. FEBS Lett 580: 6679-6684.","DOI":"10.1016\/j.febslet.2006.11.025"},{"key":"10.1124\/jpet.107.135798_bib40","doi-asserted-by":"crossref","unstructured":"Zolotarevsky Y, Hecht G, Koutsouris A, Gonzalez DE, Quan C, Tom J, Mrsny RJ, and Turner JR (2002) A membrane-permeant peptide that inhibits MLC kinase restores barrier function in in vitro models of intestinal disease. Gastroenterology 123: 163-172.","DOI":"10.1053\/gast.2002.34235"}],"container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524345823?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524345823?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/jpet.107.135798","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T22:44:07Z","timestamp":1740005047000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022356524345823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["S0022356524345823"],"URL":"https:\/\/doi.org\/10.1124\/jpet.107.135798","relation":{},"ISSN":["0022-3565"],"issn-type":[{"value":"0022-3565","type":"print"}],"subject":[],"published":{"date-parts":[[2008,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The Protein Kinase A Pathway Contributes to Hg2+-Induced Alterations in Phosphorylation and Subcellular Distribution of Occludin Associated with Increased Tight Junction Permeability of Salivary Epithelial Cell Monolayers","name":"articletitle","label":"Article Title"},{"value":"The Journal of Pharmacology and Experimental Therapeutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/jpet.107.135798","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2008 The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}