{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:01:58Z","timestamp":1791050518559,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["jpet.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"published-print":{"date-parts":[[2009,11]]},"DOI":"10.1124\/jpet.109.156513","type":"journal-article","created":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T00:45:44Z","timestamp":1250815544000},"page":"456-469","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":51,"title":["Increased Sensitivity of Glutathione S-Transferase P-Null Mice to Cyclophosphamide-Induced Urinary Bladder Toxicity"],"prefix":"10.1016","volume":"331","author":[{"given":"Daniel J.","family":"Conklin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Haberzettl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Francois","family":"Lesgards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell A.","family":"Prough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruni","family":"Bhatnagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/jpet.109.156513_bib1","doi-asserted-by":"crossref","unstructured":"Alarcon RA (1968) Fluorometric determination of acrolein and related compounds with m-aminophenol. Anal Chem 40:1704\u20131708.","DOI":"10.1021\/ac60267a019"},{"key":"10.1124\/jpet.109.156513_bib2","doi-asserted-by":"crossref","unstructured":"Batista CK, Brito GA, Souza ML, Leit\u00e3o BT, Cunha FQ, and Ribeiro RA (2006) A model of hemorrhagic cystitis induced with acrolein in mice. Braz J Med Biol Res 39:1475\u20131481.","DOI":"10.1590\/S0100-879X2006001100011"},{"key":"10.1124\/jpet.109.156513_bib3","doi-asserted-by":"crossref","unstructured":"Batista CK, Mota JM, Souza ML, Leit\u00e3o BT, Souza MH, Brito GA, Cunha FQ, and Ribeiro RA (2007) Amifostine and glutathione prevent ifosfamide- and acrolein-induced hemorrhagic cystitis. Cancer Chemother Pharmacol 59:71\u201377.","DOI":"10.1007\/s00280-006-0248-z"},{"key":"10.1124\/jpet.109.156513_bib4","doi-asserted-by":"crossref","unstructured":"Beauchamp RO, Jr, Andjelkovich DA, Kligerman AD, Morgan KT, and Heck HD (1985) A critical review of the literature on acrolein toxicity. Crit Rev Toxicol 14:309\u2013380.","DOI":"10.3109\/10408448509037461"},{"key":"10.1124\/jpet.109.156513_bib5","doi-asserted-by":"crossref","unstructured":"Berhane K, Widersten M, Engstr\u00f6m A, Kozarich JW, and Mannervik B (1994) Detoxication of base propenals and other alpha, beta-unsaturated aldehyde products of radical reactions and lipid peroxidation by human glutathione transferases. Proc Natl Acad Sci U S A 91:1480\u20131484.","DOI":"10.1073\/pnas.91.4.1480"},{"key":"10.1124\/jpet.109.156513_bib6","doi-asserted-by":"crossref","unstructured":"Bjorling DE, Elkahwaji JE, Bushman W, Janda LM, Boldon K, Hopkins WJ, and Wang ZY (2007) Acute acrolein-induced cystitis in mice. BJU Int 99:1523\u20131529.","DOI":"10.1111\/j.1464-410X.2007.06773.x"},{"key":"10.1124\/jpet.109.156513_bib7","doi-asserted-by":"crossref","unstructured":"Bjorling DE, Jerde TJ, Zine MJ, Busser BW, Saban MR, and Saban R (1999) Mast cells mediate the severity of experimental cystitis in mice. J Urol 162:231\u2013236.","DOI":"10.1097\/00005392-199907000-00073"},{"key":"10.1124\/jpet.109.156513_bib8","doi-asserted-by":"crossref","unstructured":"Board PG (2007) The use of glutathione transferase-knockout mice as pharmacological and toxicological models. Expert Opin Drug Metab Toxicol 3:421\u2013433.","DOI":"10.1517\/17425255.3.3.421"},{"key":"10.1124\/jpet.109.156513_bib9","doi-asserted-by":"crossref","unstructured":"Bon K, Lichtensteiger CA, Wilson SG, and Mogil JS (2003) Characterization of cyclophosphamide cystitis, a model of visceral and referred pain, in the mouse: species and strain differences. J Urol 170:1008\u20131012.","DOI":"10.1097\/01.ju.0000079766.49550.94"},{"key":"10.1124\/jpet.109.156513_bib10","doi-asserted-by":"crossref","unstructured":"Carmella SG, Chen M, Zhang Y, Zhang S, Hatsukami DK, and Hecht SS (2007) Quantitation of acrolein-derived (3-hydroxypropyl)mercapturic acid in human urine by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry: effects of cigarette smoking. Chem Res Toxicol 20:986\u2013990.","DOI":"10.1021\/tx700075y"},{"key":"10.1124\/jpet.109.156513_bib11","doi-asserted-by":"crossref","unstructured":"Chaviano AH, Gill WB, Ruggiero KJ, and Vermeulen CW (1985) Experimental cytoxan cystitis and prevention by acetylcysteine. J Urol 134:598\u2013600.","DOI":"10.1016\/S0022-5347(17)47309-5"},{"key":"10.1124\/jpet.109.156513_bib12","doi-asserted-by":"crossref","unstructured":"Conklin DJ, Haberzettl P, Prough RA, and Bhatnagar A (2009) Glutathione-S-transferase P protects against endothelial dysfunction induced by exposure to tobacco smoke. Am J Physiol Heart Circ Physiol 296:H1586\u2013H1597.","DOI":"10.1152\/ajpheart.00867.2008"},{"key":"10.1124\/jpet.109.156513_bib13","doi-asserted-by":"crossref","unstructured":"Cox PJ (1979) Cyclophosphamide cystitis\u2014identification of acrolein as the causative agent. Biochem Pharmacol 28:2045\u20132049.","DOI":"10.1016\/0006-2952(79)90222-3"},{"key":"10.1124\/jpet.109.156513_bib14","doi-asserted-by":"crossref","unstructured":"Ekhart C, Rodenhuis S, Smits PH, Beijnen JH, and Huitema AD (2008) Relations between polymorphisms in drug-metabolising enzymes and toxicity of chemotherapy with cyclophosphamide, thiotepa and carboplatin. Pharmacogenet Genomics 18:1009\u20131015.","DOI":"10.1097\/FPC.0b013e328313aaa4"},{"key":"10.1124\/jpet.109.156513_bib15","doi-asserted-by":"crossref","unstructured":"Elsby R, Kitteringham NR, Goldring CE, Lovatt CA, Chamberlain M, Henderson CJ, Wolf CR, and Park BK (2003) Increased constitutive c-Jun N-terminal kinase signaling in mice lacking glutathione S-transferase Pi. J Biol Chem 278:22243\u201322249.","DOI":"10.1074\/jbc.M301211200"},{"key":"10.1124\/jpet.109.156513_bib16","doi-asserted-by":"crossref","unstructured":"Gate L, Majumdar RS, Lunk A, and Tew KD (2004) Increased myeloproliferation in glutathione S-transferase pi-deficient mice is associated with a deregulation of JNK and Janus kinase\/STAT pathways. J Biol Chem 279:8608\u20138616.","DOI":"10.1074\/jbc.M308613200"},{"key":"10.1124\/jpet.109.156513_bib17","doi-asserted-by":"crossref","unstructured":"Green N, Weech M, and Walters E (2005) Localization and characterization of glutathione-S-transferase isozymes alpha, mu, and pi within the mouse vomeronasal organ. Neurosci Lett 375:198\u2013202.","DOI":"10.1016\/j.neulet.2004.11.037"},{"key":"10.1124\/jpet.109.156513_bib18","unstructured":"Gurtoo HL, Marinello AJ, Berrigan MJ, Bansal SK, Paul B, Pavelic ZP, and Struck RF (1983) Effect of thiols on toxicity and carcinostatic activity of cyclophosphamide. Semin Oncol 10:35\u201345."},{"key":"10.1124\/jpet.109.156513_bib19","doi-asserted-by":"crossref","unstructured":"Habig WH, Pabst MJ, and Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130\u20137139.","DOI":"10.1016\/S0021-9258(19)42083-8"},{"key":"10.1124\/jpet.109.156513_bib20","doi-asserted-by":"crossref","unstructured":"Hader JE, Marzella L, Myers RA, Jacobs SC, and Naslund MJ (1993) Hyperbaric oxygen treatment for experimental cyclophosphamide-induced hemorrhagic cystitis. J Urol 149:1617\u20131621.","DOI":"10.1016\/S0022-5347(17)36462-5"},{"key":"10.1124\/jpet.109.156513_bib21","doi-asserted-by":"crossref","unstructured":"Henderson CJ, Smith AG, Ure J, Brown K, Bacon EJ, and Wolf CR (1998) Increased skin tumorigenesis in mice lacking pi class glutathione S-transferases. Proc Natl Acad Sci U S A 95:5275\u20135280.","DOI":"10.1073\/pnas.95.9.5275"},{"key":"10.1124\/jpet.109.156513_bib22","doi-asserted-by":"crossref","unstructured":"Henderson CJ, Wolf CR, Kitteringham N, Powell H, Otto D, and Park BK (2000) Increased resistance to acetaminophen hepatotoxicity in mice lacking glutathione S-transferase Pi. Proc Natl Acad Sci U S A 97:12741\u201312745.","DOI":"10.1073\/pnas.220176997"},{"key":"10.1124\/jpet.109.156513_bib23","doi-asserted-by":"crossref","unstructured":"Horvath JJ, Witmer CM, and Witz G (1992) Nephrotoxicity of the 1:1 acrolein-glutathione adduct in the rat. Toxicol Appl Pharmacol 117:200\u2013207.","DOI":"10.1016\/0041-008X(92)90238-N"},{"key":"10.1124\/jpet.109.156513_bib24","unstructured":"Institute of Laboratory Animal Resources (1996) Guide for the Care and Use of Laboratory Animals, 7th ed. Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council, Washington, DC."},{"key":"10.1124\/jpet.109.156513_bib25","doi-asserted-by":"crossref","unstructured":"Korkmaz A, Topal T, and Oter S (2007) Pathophysiological aspects of cyclophosphamide and ifosfamide induced hemorrhagic cystitis; implication of reactive oxygen and nitrogen species as well as PARP activation. Cell Biol Toxicol 23:303\u2013312.","DOI":"10.1007\/s10565-006-0078-0"},{"key":"10.1124\/jpet.109.156513_bib26","doi-asserted-by":"crossref","unstructured":"Li L, Hamilton RF, Jr, Taylor DE, and Holian A (1997) Acrolein-induced cell death in human alveolar macrophages. Toxicol Appl Pharmacol 145:331\u2013339.","DOI":"10.1006\/taap.1997.8189"},{"key":"10.1124\/jpet.109.156513_bib27","doi-asserted-by":"crossref","unstructured":"Linhart I, Frant\u00edk E, Vodickov\u00e1 L, Vosmansk\u00e1 M, Smejkal J, and Mitera J (1996) Biotransformation of acrolein in rat: excretion of mercapturic acids after inhalation and intraperitoneal injection. Toxicol Appl Pharmacol 136:155\u2013160.","DOI":"10.1006\/taap.1996.0019"},{"key":"10.1124\/jpet.109.156513_bib28","unstructured":"Low JE, Borch RF, and Sladek NE (1982) Conversion of 4-hydroperoxycyclophosphamide and 4-hydroxycyclophosphamide to phosphoramide mustard and acrolein mediated by bifunctional catalysis. Cancer Res 42:830\u2013837."},{"key":"10.1124\/jpet.109.156513_bib29","doi-asserted-by":"crossref","unstructured":"Morandi P, Ruffini PA, Benvenuto GM, Raimondi R, and Fosser V (2005) Cardiac toxicity of high-dose chemotherapy. Bone Marrow Transplant 35:323\u2013334.","DOI":"10.1038\/sj.bmt.1704763"},{"key":"10.1124\/jpet.109.156513_bib30","doi-asserted-by":"crossref","unstructured":"Pal A, Hu X, Zimniak P, and Singh SV (2000) Catalytic efficiencies of allelic variants of human glutathione S-transferase Pi in the glutathione conjugation of alpha, beta-unsaturated aldehydes. Cancer Lett 154:39\u201343.","DOI":"10.1016\/S0304-3835(00)00390-6"},{"key":"10.1124\/jpet.109.156513_bib31","doi-asserted-by":"crossref","unstructured":"Parent RA, Paust DE, Schrimpf MK, Talaat RE, Doane RA, Caravello HE, Lee SJ, and Sharp DE (1998) Metabolism and distribution of [2,3\u201314C]acrolein in Sprague-Dawley rats. II. Identification of urinary and fecal metabolites. Toxicol Sci 43:110\u2013120.","DOI":"10.1093\/toxsci\/43.2.110"},{"key":"10.1124\/jpet.109.156513_bib32","doi-asserted-by":"crossref","unstructured":"Perini P, Calabrese M, Rinaldi L, and Gallo P (2007) The safety profile of cyclophosphamide in multiple sclerosis therapy. Expert Opin Drug Saf 6:183\u2013190.","DOI":"10.1517\/14740338.6.2.183"},{"key":"10.1124\/jpet.109.156513_bib33","doi-asserted-by":"crossref","unstructured":"Ramu K, Perry CS, Ahmed T, Pakenham G, and Kehrer JP (1996) Studies on the basis for the toxicity of acrolein mercapturates. Toxicol Appl Pharmacol 140:487\u2013498.","DOI":"10.1006\/taap.1996.0245"},{"key":"10.1124\/jpet.109.156513_bib34","unstructured":"Roberts JC, Francetic DJ, and Zera RT (1994) Chemoprotection against cyclophosphamide-induced urotoxicity: comparison of nine thiol protective agents. Anticancer Res 14:389\u2013395."},{"key":"10.1124\/jpet.109.156513_bib35","doi-asserted-by":"crossref","unstructured":"Sharda SV, Gulati S, Tripathi G, Jafar T, Kumar A, Sharma RK, and Agrawal S (2008) Do glutathione-S-transferase polymorphisms influence response to intravenous cyclophosphamide therapy in idiopathic nephrotic syndrome? Pediatr Nephrol 23:2001\u20132006.","DOI":"10.1007\/s00467-008-0883-1"},{"key":"10.1124\/jpet.109.156513_bib36","doi-asserted-by":"crossref","unstructured":"Shepherd JD, Pringle LE, Barnett MJ, Klingemann HG, Reece DE, and Phillips GL (1991) Mesna versus hyperhydration for the prevention of cyclophosphamide-induced hemorrhagic cystitis in bone marrow transplantation. J Clin Oncol 9:2016\u20132020.","DOI":"10.1200\/JCO.1991.9.11.2016"},{"key":"10.1124\/jpet.109.156513_bib37","doi-asserted-by":"crossref","unstructured":"Steinkellner H, Hoelzl C, Uhl M, Cavin C, Haidinger G, Gsur A, Schmid R, Kundi M, Bichler J, and Knasm\u00fcller S (2005) Coffee consumption induces GSTP in plasma and protects lymphocytes against (+\/\u2212)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide induced DNA-damage: results of controlled human intervention trials. Mutat Res 591:264\u2013275.","DOI":"10.1016\/j.mrfmmm.2005.04.016"},{"key":"10.1124\/jpet.109.156513_bib38","doi-asserted-by":"crossref","unstructured":"\u2019t Hoen PA, Rooseboom M, Bijsterbosch MK, van Berkel TJ, Vermeulen NP, and Commandeur JN (2002) Induction of glutathione-S-transferase mRNA levels by chemopreventive selenocysteine Se-conjugates. Biochem Pharmacol 63:1843\u20131849.","DOI":"10.1016\/S0006-2952(02)00987-5"},{"key":"10.1124\/jpet.109.156513_bib39","doi-asserted-by":"crossref","unstructured":"Tsai CW, Yang JJ, Chen HW, Sheen LY, and Lii CK (2005) Garlic organosulfur compounds upregulate the expression of the pi class of glutathione S-transferase in rat primary hepatocytes. J Nutr 135:2560\u20132565.","DOI":"10.1093\/jn\/135.11.2560"},{"key":"10.1124\/jpet.109.156513_bib40","doi-asserted-by":"crossref","unstructured":"Wood R, Eichel L, Messing EM, and Schwarz E (2001) Automated noninvasive measurement of cyclophosphamide-induced changes in murine micturition frequency and volume and demonstration of pharmacologic sensitivity. Urology 57:115\u2013116.","DOI":"10.1016\/S0090-4295(01)01059-7"}],"container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524386859?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524386859?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/jpet.109.156513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T01:39:53Z","timestamp":1740015593000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022356524386859"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["S0022356524386859"],"URL":"https:\/\/doi.org\/10.1124\/jpet.109.156513","relation":{},"ISSN":["0022-3565"],"issn-type":[{"value":"0022-3565","type":"print"}],"subject":[],"published":{"date-parts":[[2009,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Increased Sensitivity of Glutathione S-Transferase P-Null Mice to Cyclophosphamide-Induced Urinary Bladder Toxicity","name":"articletitle","label":"Article Title"},{"value":"The Journal of Pharmacology and Experimental Therapeutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/jpet.109.156513","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2009 by The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}