{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:10:35Z","timestamp":1792293035917,"version":"4.4.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["jpet.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"published-print":{"date-parts":[[2010,4]]},"DOI":"10.1124\/jpet.109.161182","type":"journal-article","created":{"date-parts":[[2010,1,13]],"date-time":"2010-01-13T04:15:11Z","timestamp":1263356111000},"page":"70-80","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Amelioration of Glucose Intolerance by the Synthetic Androstene HE3286: Link to Inflammatory Pathways"],"prefix":"10.1016","volume":"333","author":[{"given":"Tianlun","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Villegas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve K.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarence","family":"Ahlem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerrold M.","family":"Olefsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Reading","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Frincke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Alleva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Flores-Riveros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/jpet.109.161182_bib1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/nm1185","article-title":"IKK-\u03b2 links inflammation to obesity-induced insulin resistance.","volume":"11","author":"Arkan","year":"2005","journal-title":"Nat Med"},{"key":"10.1124\/jpet.109.161182_bib2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.autrev.2008.11.011","article-title":"7-Hydroxy androstene steroids and a novel synthetic analogue with reduced side effects as a potential agent to treat autoimmune diseases.","volume":"8","author":"Auci","year":"2009","journal-title":"Autoimmun Rev"},{"key":"10.1124\/jpet.109.161182_bib3","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1038\/nature07202","article-title":"Integration of metabolism and inflammation by lipid-activated nuclear receptors.","volume":"454","author":"Bensinger","year":"2008","journal-title":"Nature"},{"key":"10.1124\/jpet.109.161182_bib4","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.4049\/jimmunol.175.8.5370","article-title":"Discovery and pharmacological characterization of a novel rodent-active CCR2 antagonist, INCB3344.","volume":"175","author":"Brodmerkel","year":"2005","journal-title":"J Immunol"},{"key":"10.1124\/jpet.109.161182_bib5","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1210\/jc.2004-1696","article-title":"Monocyte chemoattractant protein-1 release is higher in visceral than subcutaneous human adipose tissue (AT): implication of macrophages resident in the AT.","volume":"90","author":"Bruun","year":"2005","journal-title":"J Clin Endocrinol Metab"},{"key":"10.1124\/jpet.109.161182_bib6","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/nm1166","article-title":"Local and systemic insulin resistance resulting from hepatic activation of IKK-\u03b2 and NF-\u03baB.","volume":"11","author":"Cai","year":"2005","journal-title":"Nat Med"},{"key":"10.1124\/jpet.109.161182_bib7","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1161\/01.RES.0000146672.10582.17","article-title":"Chemokines in the pathogenesis of vascular disease.","volume":"95","author":"Charo","year":"2004","journal-title":"Circ Res"},{"key":"10.1124\/jpet.109.161182_bib8","doi-asserted-by":"crossref","first-page":"26","DOI":"10.2337\/diab.33.1.26","article-title":"Effect of genetic background on the therapeutic effects of dehydroepiandrosterone (DHEA) in diabetes-obesity mutants and in aged normal mice.","volume":"33","author":"Coleman","year":"1984","journal-title":"Diabetes"},{"key":"10.1124\/jpet.109.161182_bib9","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1093\/embo-reports\/kve007","article-title":"The orphan nuclear receptor ROR\u03b1 is a negative regulator of the inflammatory response.","volume":"2","author":"Delerive","year":"2001","journal-title":"EMBO Rep"},{"key":"10.1124\/jpet.109.161182_bib10","doi-asserted-by":"crossref","first-page":"112","DOI":"10.2144\/04371RR03","article-title":"Validation of housekeeping genes for normalizing RNA expression in real-time PCR.","volume":"37","author":"Dheda","year":"2004","journal-title":"BioTechniques"},{"key":"10.1124\/jpet.109.161182_bib11","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/j.1752-8062.2008.00026.x","article-title":"Use of salsalate to target inflammation in the treatment of insulin resistance and type 2 diabetes.","volume":"1","author":"Goldfine","year":"2008","journal-title":"Clin Transl Sci"},{"key":"10.1124\/jpet.109.161182_bib12","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1172\/JCI31561","article-title":"Macrophage PPAR\u03b3 is required for normal skeletal muscle and hepatic insulin sensitivity and full antidiabetic effects of thiazolidinediones.","volume":"117","author":"Hevener","year":"2007","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib13","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nature01137","article-title":"A central role for JNK in obesity and insulin resistance.","volume":"420","author":"Hirosumi","year":"2002","journal-title":"Nature"},{"key":"10.1124\/jpet.109.161182_bib14","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1172\/JCI117936","article-title":"Increased adipose tissue expression of tumor necrosis factor-\u03b1 in human obesity and insulin resistance.","volume":"95","author":"Hotamisligil","year":"1995","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib15","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1172\/JCI483","article-title":"NF\u03baB prevents apoptosis and liver dysfunction during liver regeneration.","volume":"101","author":"Iimuro","year":"1998","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib16","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1172\/JCI26498","article-title":"MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity.","volume":"116","author":"Kanda","year":"2006","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib17","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1172\/JCI117899","article-title":"The expression of tumor necrosis factor in human adipose tissue. Regulation by obesity, weight loss, and relationship to lipoprotein lipase.","volume":"95","author":"Kern","year":"1995","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib18","doi-asserted-by":"crossref","first-page":"36828","DOI":"10.1074\/jbc.M404927200","article-title":"ROR\u03b1 regulates the expression of genes involved in lipid homeostasis in skeletal muscle cells: caveolin-3 and CPT-1 are direct targets of ROR.","volume":"279","author":"Lau","year":"2004","journal-title":"J Biol Chem"},{"key":"10.1124\/jpet.109.161182_bib19","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/0026-0495(87)90095-3","article-title":"Androgenic and estrogenic metabolites in serum of mice fed dehydroepiandrosterone: relationship to antihyperglycemic effects.","volume":"36","author":"Leiter","year":"1987","journal-title":"Metabolism"},{"key":"10.1124\/jpet.109.161182_bib20","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-\u0394\u0394C(T)) method.","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1124\/jpet.109.161182_bib21","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S1570-0232(01)00570-0","article-title":"Ergosteroids. VI. Metabolism of dehydroepiandrosterone by rat liver in vitro: a liquid chromatographic-mass spectrometric study.","volume":"767","author":"Marwah","year":"2002","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"10.1124\/jpet.109.161182_bib22","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/35100529","article-title":"Toll-like receptors and innate immunity.","volume":"1","author":"Medzhitov","year":"2001","journal-title":"Nat Rev Immunol"},{"key":"10.1124\/jpet.109.161182_bib23","doi-asserted-by":"crossref","first-page":"6570","DOI":"10.1073\/pnas.0401401101","article-title":"Err\u03b1 and Gabpa\/b specify PGC-1\u03b1-dependent oxidative phosphorylation gene expression that is altered in diabetic muscle.","volume":"101","author":"Mootha","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/jpet.109.161182_bib24","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1056\/NEJMoa054629","article-title":"DHEA in elderly women and DHEA or testosterone in elderly men.","volume":"355","author":"Nair","year":"2006","journal-title":"N Engl J Med"},{"key":"10.1124\/jpet.109.161182_bib25","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1517\/14728222.11.11.1503","article-title":"Macrophage peroxisome proliferator activated receptor \u03b3 as a therapeutic target to combat type 2 diabetes.","volume":"11","author":"Pascual","year":"2007","journal-title":"Exp Opin Ther Targets"},{"key":"10.1124\/jpet.109.161182_bib26","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR.","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"10.1124\/jpet.109.161182_bib27","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/34178","article-title":"The peroxisome proliferator-activated receptor-\u03b3 is a negative regulator of macrophage activation.","volume":"391","author":"Ricote","year":"1998","journal-title":"Nature"},{"key":"10.1124\/jpet.109.161182_bib28","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1073\/pnas.1133870100","article-title":"Monocyte chemoattractant protein 1 in obesity and insulin resistance.","volume":"100","author":"Sartipy","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/jpet.109.161182_bib29","doi-asserted-by":"crossref","first-page":"2992","DOI":"10.1172\/JCI34260","article-title":"Insulin sensitivity: modulation by nutrients and inflammation.","volume":"118","author":"Schenk","year":"2008","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib30","doi-asserted-by":"crossref","first-page":"3015","DOI":"10.1172\/JCI28898","article-title":"TLR4 links innate immunity and fatty acid-induced insulin resistance.","volume":"116","author":"Shi","year":"2006","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib31","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.1053\/j.gastro.2007.03.059","article-title":"Obesity, inflammation, and insulin resistance.","volume":"132","author":"Shoelson","year":"2007","journal-title":"Gastroenterology"},{"key":"10.1124\/jpet.109.161182_bib32","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.cmet.2007.09.011","article-title":"JNK1 in hematopoietically derived cells contributes to diet-induced inflammation and insulin resistance without affecting obesity.","volume":"6","author":"Solinas","year":"2007","journal-title":"Cell Metab"},{"key":"10.1124\/jpet.109.161182_bib33","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s00125-006-0467-1","article-title":"Amelioration of glucose tolerance by hepatic inhibition of nuclear factor \u03baB in db\/db mice.","volume":"50","author":"Tamura","year":"2007","journal-title":"Diabetologia"},{"key":"10.1124\/jpet.109.161182_bib34","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1172\/JCI24335","article-title":"CCR2 modulates inflammatory and metabolic effects of high-fat feeding.","volume":"116","author":"Weisberg","year":"2006","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib35","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1172\/JCI200319246","article-title":"Obesity is associated with macrophage accumulation in adipose tissue.","volume":"112","author":"Weisberg","year":"2003","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib36","doi-asserted-by":"crossref","first-page":"6712","DOI":"10.1073\/pnas.1031789100","article-title":"PPAR\u03b3 and PPAR\u03b4 negatively regulate specific subsets of lipopolysaccharide and IFN-\u03b3 target genes in macrophages.","volume":"100","author":"Welch","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/jpet.109.161182_bib37","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/toxsci\/66.1.69","article-title":"A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists.","volume":"66","author":"Wilson","year":"2002","journal-title":"Toxicol Sci"},{"key":"10.1124\/jpet.109.161182_bib38","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/toxsci\/kfh180","article-title":"Development and characterization of a cell line that stably expresses an estrogen-responsive luciferase reporter for the detection of estrogen receptor agonist and antagonists.","volume":"81","author":"Wilson","year":"2004","journal-title":"Toxicol Sci"},{"key":"10.1124\/jpet.109.161182_bib39","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1172\/JCI200319451","article-title":"Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance.","volume":"112","author":"Xu","year":"2003","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.109.161182_bib40","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1126\/science.1061620","article-title":"Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of Ikk\u03b2.","volume":"293","author":"Yuan","year":"2001","journal-title":"Science"}],"container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524457339?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524457339?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/jpet.109.161182","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T02:09:03Z","timestamp":1740017343000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022356524457339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["S0022356524457339"],"URL":"https:\/\/doi.org\/10.1124\/jpet.109.161182","relation":{},"ISSN":["0022-3565"],"issn-type":[{"value":"0022-3565","type":"print"}],"subject":[],"published":{"date-parts":[[2010,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Amelioration of Glucose Intolerance by the Synthetic Androstene HE3286: Link to Inflammatory Pathways","name":"articletitle","label":"Article Title"},{"value":"The Journal of Pharmacology and Experimental Therapeutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/jpet.109.161182","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2010 by The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}