{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:30:07Z","timestamp":1771655407462,"version":"3.50.1"},"reference-count":57,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":3136,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["jpet.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1124\/jpet.116.232009","type":"journal-article","created":{"date-parts":[[2016,3,26]],"date-time":"2016-03-26T01:25:47Z","timestamp":1458955547000},"page":"606-618","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"title":["Tiotropium Attenuates Virus-Induced Pulmonary Inflammation in Cigarette Smoke\u2013Exposed Mice"],"prefix":"10.1016","volume":"357","author":[{"given":"Hannes","family":"Bucher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias J.","family":"Duechs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Tilp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus J.","family":"Erb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1124\/jpet.116.232009_bib1","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1183\/09031936.00040709","article-title":"Inhibition of neutrophil elastase-induced goblet cell metaplasia by tiotropium in mice","volume":"35","author":"Arai","year":"2010","journal-title":"Eur Respir J"},{"key":"10.1124\/jpet.116.232009_bib2","doi-asserted-by":"crossref","first-page":"781","DOI":"10.2147\/COPD.S3945","article-title":"Suppressive activity of tiotropium bromide on matrix metalloproteinase production from lung fibroblasts in vitro","volume":"3","author":"Asano","year":"2008","journal-title":"Int J Chron Obstruct Pulmon Dis"},{"key":"10.1124\/jpet.116.232009_bib3","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/nrd820","article-title":"New treatments for COPD","volume":"1","author":"Barnes","year":"2002","journal-title":"Nat Rev Drug Discov"},{"key":"10.1124\/jpet.116.232009_bib4","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1513\/pats.200402-014MS","article-title":"Corticosteroid resistance in airway disease","volume":"1","author":"Barnes","year":"2004","journal-title":"Proc Am Thorac Soc"},{"key":"10.1124\/jpet.116.232009_bib5","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.jaci.2012.12.1564","article-title":"Corticosteroid resistance in patients with asthma and chronic obstructive pulmonary disease","volume":"131","author":"Barnes","year":"2013","journal-title":"J Allergy Clin Immunol"},{"key":"10.1124\/jpet.116.232009_bib6","doi-asserted-by":"crossref","first-page":"3546","DOI":"10.1172\/JCI36130","article-title":"The cytokine network in asthma and chronic obstructive pulmonary disease","volume":"118","author":"Barnes","year":"2008","journal-title":"J Clin Invest"},{"key":"10.1124\/jpet.116.232009_bib7","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.pupt.2009.06.002","article-title":"Alternative mechanisms for tiotropium","volume":"22","author":"Bateman","year":"2009","journal-title":"Pulm Pharmacol Ther"},{"key":"10.1124\/jpet.116.232009_bib8","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1183\/09031936.00004907","article-title":"Inhibition of allergen-induced airway remodelling by tiotropium and budesonide: a comparison","volume":"30","author":"Bos","year":"2007","journal-title":"Eur Respir J"},{"key":"10.1124\/jpet.116.232009_bib9","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.pupt.2013.09.004","article-title":"Tiotropium bromide inhibits relapsing allergic asthma in BALB\/c mice","volume":"27","author":"Bosnjak","year":"2014","journal-title":"Pulm Pharmacol Ther"},{"key":"10.1124\/jpet.116.232009_bib10","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1111\/j.1476-5381.2011.01632.x","article-title":"Non-bronchodilating mechanisms of tiotropium prevent airway hyperreactivity in a guinea-pig model of allergic asthma","volume":"165","author":"Buels","year":"2012","journal-title":"Br J Pharmacol"},{"key":"10.1124\/jpet.116.232009_bib11","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1016\/j.rmed.2007.06.009","article-title":"Tiotropium suppresses acetylcholine-induced release of chemotactic mediators in vitro","volume":"101","author":"B\u00fchling","year":"2007","journal-title":"Respir Med"},{"key":"10.1124\/jpet.116.232009_bib12","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1056\/NEJMoa063070","article-title":"Salmeterol and fluticasone propionate and survival in chronic obstructive pulmonary disease","volume":"356","author":"Calverley","year":"2007","journal-title":"N Engl J Med"},{"key":"10.1124\/jpet.116.232009_bib13","doi-asserted-by":"crossref","first-page":"2040","DOI":"10.1128\/JVI.01625-07","article-title":"Viral and host factors in human respiratory syncytial virus pathogenesis","volume":"82","author":"Collins","year":"2008","journal-title":"J Virol"},{"key":"10.1124\/jpet.116.232009_bib14","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0149-2918(01)80113-2","article-title":"A review of the pharmacology and pharmacokinetics of inhaled fluticasone propionate and mometasone furoate","volume":"23","author":"Crim","year":"2001","journal-title":"Clin Ther"},{"key":"10.1124\/jpet.116.232009_bib15","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1183\/09031936.00139909","article-title":"Tiotropium reduction of lung inflammation in a model of chronic gastro-oesophageal reflux","volume":"35","author":"Cui","year":"2010","journal-title":"Eur Respir J"},{"key":"10.1124\/jpet.116.232009_bib16","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1038\/489S2a","article-title":"Health impact: breathless","volume":"489","author":"Dance","year":"2012","journal-title":"Nature"},{"key":"10.1124\/jpet.116.232009_bib17","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/S0024-3205(98)00588-8","article-title":"Tiotropium (Spiriva): mechanistical considerations and clinical profile in obstructive lung disease","volume":"64","author":"Disse","year":"1999","journal-title":"Life Sci"},{"key":"10.1124\/jpet.116.232009_bib18","doi-asserted-by":"crossref","first-page":"e91223","DOI":"10.1371\/journal.pone.0091223","article-title":"Development of a novel severe triple allergen asthma model in mice which is resistant to dexamethasone and partially resistant to TLR7 and TLR9 agonist treatment","volume":"9","author":"Duechs","year":"2014","journal-title":"PLoS One"},{"key":"10.1124\/jpet.116.232009_bib19","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1164\/rccm.200409-1249OC","article-title":"Protective effects of tiotropium bromide in the progression of airway smooth muscle remodeling","volume":"171","author":"Gosens","year":"2005","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1124\/jpet.116.232009_bib20","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1465-9921-7-73","article-title":"Muscarinic receptor signaling in the pathophysiology of asthma and COPD","volume":"7","author":"Gosens","year":"2006","journal-title":"Respir Res"},{"key":"10.1124\/jpet.116.232009_bib21","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1164\/ajrccm.156.3.9606031","article-title":"Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography","volume":"156","author":"Hamelmann","year":"1997","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1124\/jpet.116.232009_bib22","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0022-3565(24)29537-9","article-title":"Anti-inflammatory and immunomodulatory potential of the novel PDE4 inhibitor roflumilast in vitro","volume":"297","author":"Hatzelmann","year":"2001","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/jpet.116.232009_bib23","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1159\/000151729","article-title":"Tiotropium bromide attenuates respiratory syncytial virus replication in epithelial cells","volume":"76","author":"Iesato","year":"2008","journal-title":"Respiration"},{"issue":"5, Suppl 1","key":"10.1124\/jpet.116.232009_bib24","doi-asserted-by":"crossref","first-page":"251S","DOI":"10.1378\/chest.117.5_suppl_1.251S","article-title":"Comparison of the structural and inflammatory features of COPD and asthma. Giles F. Filley Lecture","volume":"117","author":"Jeffery","year":"2000","journal-title":"Chest"},{"key":"10.1124\/jpet.116.232009_bib25","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1513\/pats.200603-075MS","article-title":"IFN-gamma-dependent DNA injury and\/or apoptosis are critical in cigarette smoke-induced murine emphysema","volume":"3","author":"Kang","year":"2006","journal-title":"Proc Am Thorac Soc"},{"key":"10.1124\/jpet.116.232009_bib26","doi-asserted-by":"crossref","first-page":"409580","DOI":"10.1155\/2012\/409580","article-title":"Muscarinic receptors and their antagonists in COPD: anti-inflammatory and antiremodeling effects","volume":"2012","author":"Karakiulakis","year":"2012","journal-title":"Mediators Inflamm"},{"key":"10.1124\/jpet.116.232009_bib27","doi-asserted-by":"crossref","first-page":"247","DOI":"10.2165\/00151829-200403040-00005","article-title":"Tiotropium bromide: a review of its use as maintenance therapy in patients with COPD","volume":"3","author":"Keam","year":"2004","journal-title":"Treat Respir Med"},{"key":"10.1124\/jpet.116.232009_bib28","first-page":"681258","article-title":"Cholinergic regulation of airway inflammation and remodelling","volume":"2012","author":"Kolahian","year":"2012","journal-title":"J Allergy (Cairo)"},{"key":"10.1124\/jpet.116.232009_bib29","doi-asserted-by":"crossref","first-page":"17869","DOI":"10.1074\/jbc.M000217200","article-title":"Cloning and expression of the human N-acetylneuraminic acid phosphate synthase gene with 2-keto-3-deoxy-D-glycero- D-galacto-nononic acid biosynthetic ability","volume":"275","author":"Lawrence","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1124\/jpet.116.232009_bib30","first-page":"5","article-title":"Fluticasone induces epithelial injury and alters barrier function in normal subjects","volume":"5","author":"MacRedmond","year":"2014","journal-title":"J Steroids Horm Sci"},{"key":"10.1124\/jpet.116.232009_bib31","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1056\/NEJMoa1407154","article-title":"Withdrawal of inhaled glucocorticoids and exacerbations of COPD","volume":"371","author":"Magnussen","year":"2014","journal-title":"N Engl J Med"},{"key":"10.1124\/jpet.116.232009_bib32","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1586\/ers.09.19","article-title":"Tiotropium bromide for chronic obstructive pulmonary disease","volume":"3","author":"Mamary","year":"2009","journal-title":"Expert Rev Respir Med"},{"key":"10.1124\/jpet.116.232009_bib33","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1465-9921-14-55","article-title":"Rate of progression of CT-quantified emphysema in male current and ex-smokers: a follow-up study","volume":"14","author":"Mohamed Hoesein","year":"2013","journal-title":"Respir Res"},{"key":"10.1124\/jpet.116.232009_bib34","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/nrd1472","article-title":"Tiotropium bromide","volume":"3","author":"Mundy","year":"2004","journal-title":"Nat Rev Drug Discov"},{"key":"10.1124\/jpet.116.232009_bib35","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1177\/1753465812466167","article-title":"Efficacy and safety of roflumilast in patients with chronic obstructive pulmonary disease: a systematic review and meta-analysis","volume":"7","author":"Oba","year":"2013","journal-title":"Ther Adv Respir Dis"},{"key":"10.1124\/jpet.116.232009_bib36","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1111\/j.1365-2222.2010.03478.x","article-title":"Effect of tiotropium bromide on airway inflammation and remodelling in a mouse model of asthma","volume":"40","author":"Ohta","year":"2010","journal-title":"Clin Exp Allergy"},{"key":"10.1124\/jpet.116.232009_bib37","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1513\/pats.200512-125SF","article-title":"Pathophysiology of exacerbations of chronic obstructive pulmonary disease","volume":"3","author":"Papi","year":"2006","journal-title":"Proc Am Thorac Soc"},{"key":"10.1124\/jpet.116.232009_bib38","doi-asserted-by":"crossref","first-page":"e6843","DOI":"10.1136\/bmj.e6843","article-title":"Inhaled corticosteroids for chronic obstructive pulmonary disease","volume":"345","author":"Park","year":"2012","journal-title":"BMJ"},{"key":"10.1124\/jpet.116.232009_bib39","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1476-5381.2011.01218.x","article-title":"Update on roflumilast, a phosphodiesterase 4 inhibitor for the treatment of chronic obstructive pulmonary disease","volume":"163","author":"Rabe","year":"2011","journal-title":"Br J Pharmacol"},{"key":"10.1124\/jpet.116.232009_bib40","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1164\/rccm.200703-456SO","article-title":"Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary","volume":"176","author":"Rabe","year":"2007","journal-title":"Am J Respir Crit Care Med"},{"key":"10.1124\/jpet.116.232009_bib41","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ejphar.2005.12.050","article-title":"Control by cholinergic mechanisms","volume":"533","author":"Rack\u00e9","year":"2006","journal-title":"Eur J Pharmacol"},{"key":"10.1124\/jpet.116.232009_bib42","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.pupt.2004.03.001","article-title":"The airway cholinergic system: physiology and pharmacology","volume":"17","author":"Rack\u00e9","year":"2004","journal-title":"Pulm Pharmacol Ther"},{"key":"10.1124\/jpet.116.232009_bib43","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/1465-9921-12-18","article-title":"Reduction of exacerbations by the PDE4 inhibitor roflumilast--the importance of defining different subsets of patients with COPD","volume":"12","author":"Rennard","year":"2011","journal-title":"Respir Res"},{"issue":"3, Suppl","key":"10.1124\/jpet.116.232009_bib44","doi-asserted-by":"crossref","first-page":"S68","DOI":"10.1016\/j.jamda.2005.03.015","article-title":"Corticosteroids and chronic obstructive pulmonary disease in the nursing home","volume":"6","author":"Rich","year":"2005","journal-title":"J Am Med Dir Assoc"},{"key":"10.1124\/jpet.116.232009_bib45","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1136\/thx.2005.041863","article-title":"COPD exacerbations. 5: management","volume":"61","author":"Rodr\u00edguez-Roisin","year":"2006","journal-title":"Thorax"},{"key":"10.1124\/jpet.116.232009_bib46","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s15010-008-7327-5","article-title":"Inflammatory response in acute viral exacerbations of COPD","volume":"36","author":"Rohde","year":"2008","journal-title":"Infection"},{"key":"10.1124\/jpet.116.232009_bib47","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1136\/thx.2005.041822","article-title":"COPD exacerbations. 2: aetiology","volume":"61","author":"Sapey","year":"2006","journal-title":"Thorax"},{"key":"10.1124\/jpet.116.232009_bib48","doi-asserted-by":"crossref","first-page":"9609","DOI":"10.1021\/bi00155a013","article-title":"Binding of influenza virus hemagglutinin to analogs of its cell-surface receptor, sialic acid: analysis by proton nuclear magnetic resonance spectroscopy and X-ray crystallography","volume":"31","author":"Sauter","year":"1992","journal-title":"Biochemistry"},{"key":"10.1124\/jpet.116.232009_bib49","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1001\/archinternmed.2008.550","article-title":"Long-term use of inhaled corticosteroids and the risk of pneumonia in chronic obstructive pulmonary disease: a meta-analysis","volume":"169","author":"Singh","year":"2009","journal-title":"Arch Intern Med"},{"key":"10.1124\/jpet.116.232009_bib50","doi-asserted-by":"crossref","first-page":"11303","DOI":"10.1128\/JVI.77.21.11303-11311.2003","article-title":"Inability to evoke a long-lasting protective immune response to respiratory syncytial virus infection in mice correlates with ineffective nasal antibody responses","volume":"77","author":"Singleton","year":"2003","journal-title":"J Virol"},{"key":"10.1124\/jpet.116.232009_bib51","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1513\/pats.200904-016DS","article-title":"Burden and pathogenesis of chronic obstructive pulmonary disease","volume":"6","author":"Stockley","year":"2009","journal-title":"Proc Am Thorac Soc"},{"key":"10.1124\/jpet.116.232009_bib52","first-page":"439","article-title":"Suppression of IL-8 production from airway cells by tiotropium bromide in vitro","volume":"6","author":"Suzaki","year":"2011","journal-title":"Int J Chron Obstruct Pulmon Dis"},{"key":"10.1124\/jpet.116.232009_bib53","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1056\/NEJMoa0805800","article-title":"A 4-year trial of tiotropium in chronic obstructive pulmonary disease","volume":"359","author":"Tashkin","year":"2008","journal-title":"N Engl J Med"},{"key":"10.1124\/jpet.116.232009_bib54","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1080\/09629359890910","article-title":"Glucocorticoids: mechanisms of action and anti-inflammatory potential in asthma","volume":"7","author":"van der Velden","year":"1998","journal-title":"Mediators Inflamm"},{"key":"10.1124\/jpet.116.232009_bib55","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1056\/NEJMoa1008378","article-title":"Tiotropium versus salmeterol for the prevention of exacerbations of COPD","volume":"364","author":"Vogelmeier","year":"2011","journal-title":"N Engl J Med"},{"key":"10.1124\/jpet.116.232009_bib56","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/S0140-6736(07)61382-8","article-title":"COPD exacerbations: defining their cause and prevention","volume":"370","author":"Wedzicha","year":"2007","journal-title":"Lancet"},{"key":"10.1124\/jpet.116.232009_bib57","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.pupt.2010.03.008","article-title":"Tiotropium bromide exerts anti-inflammatory activity in a cigarette smoke mouse model of COPD","volume":"23","author":"Wollin","year":"2010","journal-title":"Pulm Pharmacol Ther"}],"container-title":["The Journal of Pharmacology and Experimental Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524193317?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022356524193317?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/jpet.116.232009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T17:18:10Z","timestamp":1740763090000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022356524193317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":57,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["S0022356524193317"],"URL":"https:\/\/doi.org\/10.1124\/jpet.116.232009","relation":{},"ISSN":["0022-3565"],"issn-type":[{"value":"0022-3565","type":"print"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Tiotropium Attenuates Virus-Induced Pulmonary Inflammation in Cigarette Smoke\u2013Exposed Mice","name":"articletitle","label":"Article Title"},{"value":"The Journal of Pharmacology and Experimental Therapeutics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/jpet.116.232009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Author(s)","name":"copyright","label":"Copyright"}]}}