{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T05:42:11Z","timestamp":1788846131540,"version":"build-2803163510"},"reference-count":57,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["molpharm.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Pharmacology"],"published-print":{"date-parts":[[2018,11]]},"DOI":"10.1124\/mol.118.113357","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T12:25:38Z","timestamp":1536236738000},"page":"1270-1279","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Activation of Caenorhabditis elegans Levamisole-Sensitive and Mammalian Nicotinic Receptors by the Antiparasitic Bephenium"],"prefix":"10.1016","volume":"94","author":[{"given":"Ornella","family":"Turani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermina","family":"Hernando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerem\u00edas","family":"Corradi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Bouzat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1124\/mol.118.113357_bib1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1113\/jphysiol.2003.043885","article-title":"Activation and block of mouse muscle-type nicotinic receptors by tetraethylammonium","volume":"551","author":"Akk","year":"2003","journal-title":"J Physiol"},{"key":"10.1124\/mol.118.113357_bib2","doi-asserted-by":"crossref","first-page":"9173","DOI":"10.1073\/pnas.1116397109","article-title":"Molecular actions of smoking cessation drugs at \u03b14\u03b22 nicotinic receptors defined in crystal structures of a homologous binding protein","volume":"109","author":"Billen","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/mol.118.113357_bib3","article-title":"Caenorhabditis elegans and parasitic species","volume":"14","author":"Blanchard","year":"2018","journal-title":"PLoS Pathog"},{"key":"10.1124\/mol.118.113357_bib4","doi-asserted-by":"crossref","first-page":"18590","DOI":"10.1073\/pnas.0806933105","article-title":"Caenorhabditis elegans levamisole-sensitive acetylcholine receptor","volume":"105","author":"Boulin","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/mol.118.113357_bib5","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1111\/j.1476-5381.2011.01420.x","article-title":"Haemonchus contortus Xenopus oocytes provides mechanistic insights into levamisole resistance","volume":"164","author":"Boulin","year":"2011","journal-title":"Br J Pharmacol"},{"key":"10.1124\/mol.118.113357_bib6","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1085\/jgp.115.5.663","article-title":"Nicotinic receptor fourth transmembrane domain: hydrogen bonding by conserved threonine contributes to channel gating kinetics","volume":"115","author":"Bouzat","year":"2000","journal-title":"J Gen Physiol"},{"key":"10.1124\/mol.118.113357_bib7","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1016\/0896-6273(94)90424-3","article-title":"Structural basis of the different gating kinetics of fetal and adult acetylcholine receptors","volume":"13","author":"Bouzat","year":"1994","journal-title":"Neuron"},{"key":"10.1124\/mol.118.113357_bib8","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cophys.2018.04.008","article-title":"The nicotinic acetylcholine receptor as a molecular machine for neuromuscular transmission","volume":"4","author":"Bouzat","year":"2018","journal-title":"Curr Opin Physiol"},{"key":"10.1124\/mol.118.113357_bib9","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1111\/bph.13770","article-title":"Nicotinic acetylcholine receptors at the single-channel level","volume":"175","author":"Bouzat","year":"2018","journal-title":"Br J Pharmacol"},{"key":"10.1124\/mol.118.113357_bib10","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.ijpara.2006.01.016","article-title":"Caenorhabditis elegans functional genetics to antiparasitic drug target identification and validation: nicotinic acetylcholine receptors, a case study","volume":"36","author":"Brown","year":"2006","journal-title":"Int J Parasitol"},{"key":"10.1124\/mol.118.113357_bib11","doi-asserted-by":"crossref","first-page":"607","DOI":"10.2307\/3274544","article-title":"Ancylostoma caninum","volume":"44","author":"Burrows","year":"1958","journal-title":"J Parasitol"},{"key":"10.1124\/mol.118.113357_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003870","article-title":"Investigation of acetylcholine receptor diversity in a nematode parasite leads to characterization of tribendimidine- and derquantel-sensitive nAChRs","volume":"10","author":"Buxton","year":"2014","journal-title":"PLoS Pathog"},{"issue":"Pt B","key":"10.1124\/mol.118.113357_bib13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.neuropharm.2014.12.006","article-title":"The nicotinic acetylcholine receptor and its prokaryotic homologues: structure, conformational transitions & allosteric modulation","volume":"96","author":"Cecchini","year":"2015","journal-title":"Neuropharmacology"},{"key":"10.1124\/mol.118.113357_bib14","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/S0896-6273(04)00115-1","article-title":"Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures","volume":"41","author":"Celie","year":"2004","journal-title":"Neuron"},{"key":"10.1124\/mol.118.113357_bib15","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s10158-012-0130-0","article-title":"Haemonchus contortus levamisole-sensitive acetylcholine receptors","volume":"12","author":"Charvet","year":"2012","journal-title":"Invert Neurosci"},{"key":"10.1124\/mol.118.113357_bib16","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0896-6273(02)00591-3","article-title":"C. elegans neurons and muscle cells","volume":"33","author":"Christensen","year":"2002","journal-title":"Neuron"},{"key":"10.1124\/mol.118.113357_bib17","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/181183a0","article-title":"A new series of anthelmintics","volume":"181","author":"Copp","year":"1958","journal-title":"Nature"},{"key":"10.1124\/mol.118.113357_bib18","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1016\/j.bpj.2009.06.018","article-title":"Single-channel kinetic analysis for activation and desensitization of homomeric 5-HT(3)A receptors","volume":"97","author":"Corradi","year":"2009","journal-title":"Biophys J"},{"key":"10.1124\/mol.118.113357_bib19","doi-asserted-by":"crossref","first-page":"42476","DOI":"10.1074\/jbc.M404370200","article-title":"Caenorhabditis elegans unc-63 gene encodes a levamisole-sensitive nicotinic acetylcholine receptor \u03b1 subunit","volume":"279","author":"Culetto","year":"2004","journal-title":"J Biol Chem"},{"issue":"6 Suppl 1","key":"10.1124\/mol.118.113357_bib20","doi-asserted-by":"crossref","first-page":"1504S","DOI":"10.1093\/jn\/137.6.1504S","article-title":"Cation-\u03c0 interactions involving aromatic amino acids","volume":"137","author":"Dougherty","year":"2007","journal-title":"J Nutr"},{"key":"10.1124\/mol.118.113357_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004826","article-title":"Recent duplication and functional divergence in parasitic nematode levamisole-sensitive acetylcholine receptors","volume":"10","author":"Duguet","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1124\/mol.118.113357_bib22","doi-asserted-by":"crossref","first-page":"5843","DOI":"10.1523\/JNEUROSCI.17-15-05843.1997","article-title":"Caenorhabditis elegans lev-1, unc-29 unc-38 encode functional nicotinic acetylcholine receptor subunits","volume":"17","author":"Fleming","year":"1997","journal-title":"J Neurosci"},{"key":"10.1124\/mol.118.113357_bib23","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1016\/S0028-3908(03)00247-8","article-title":"Molecular mechanisms of inhibition of nicotinic acetylcholine receptors by tricyclic antidepressants","volume":"45","author":"Gumilar","year":"2003","journal-title":"Neuropharmacology"},{"key":"10.1124\/mol.118.113357_bib24","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1124\/mol.112.079962","article-title":"Caenorhabditis elegans levamisole-sensitive nicotinic receptor function","volume":"82","author":"Hernando","year":"2012","journal-title":"Mol Pharmacol"},{"key":"10.1124\/mol.118.113357_bib25","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.1038\/emboj.2009.227","article-title":"Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor","volume":"28","author":"Hibbs","year":"2009","journal-title":"EMBO J"},{"key":"10.1124\/mol.118.113357_bib26","doi-asserted-by":"crossref","unstructured":"Holden-Dye L and Walker RJ (2007) Anthelmintic drugs. WormBook, ed. The C. elegans Research Community, WormBook, doi\/10.1895\/wormbook.1.143.1, http:\/\/www.wormbook.org.","DOI":"10.1895\/wormbook.1.143.1"},{"key":"10.1124\/mol.118.113357_bib27","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s10158-007-0049-z","article-title":"The nicotinic acetylcholine receptor gene family of the nematode Caenorhabditis elegans: an update on nomenclature","volume":"7","author":"Jones","year":"2007","journal-title":"Invert Neurosci"},{"key":"10.1124\/mol.118.113357_bib28","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s10158-008-0068-4","article-title":"The cys-loop ligand-gated ion channel gene superfamily of the nematode, Caenorhabditis elegans","volume":"8","author":"Jones","year":"2008","journal-title":"Invert Neurosci"},{"key":"10.1124\/mol.118.113357_bib29","doi-asserted-by":"crossref","first-page":"3799","DOI":"10.1111\/j.1742-4658.2007.05935.x","article-title":"Muscle and neuronal nicotinic acetylcholine receptors. Structure, function and pathogenicity","volume":"274","author":"Kalamida","year":"2007","journal-title":"FEBS J"},{"key":"10.1124\/mol.118.113357_bib30","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1093\/genetics\/95.4.905","article-title":"Caenorhabditis elegans","volume":"95","author":"Lewis","year":"1980","journal-title":"Genetics"},{"issue":"Suppl","key":"10.1124\/mol.118.113357_bib31","doi-asserted-by":"crossref","first-page":"S111","DOI":"10.1017\/S0031182097001029","article-title":"Target sites of anthelmintics","volume":"114","author":"Martin","year":"1997","journal-title":"Parasitology"},{"key":"10.1124\/mol.118.113357_bib32","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.tips.2009.12.005","article-title":"Binding, activation and modulation of Cys-loop receptors","volume":"31","author":"Miller","year":"2010","journal-title":"Trends Pharmacol Sci"},{"key":"10.1124\/mol.118.113357_bib33","first-page":"213","article-title":"Caenorhabditis elegans","volume":"6","author":"Mongan","year":"1998","journal-title":"Receptors Channels"},{"key":"10.1124\/mol.118.113357_bib34","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/nature19785","article-title":"X-ray structure of the human \u03b14\u03b22 nicotinic receptor","volume":"538","author":"Morales-Perez","year":"2016","journal-title":"Nature"},{"key":"10.1124\/mol.118.113357_bib35","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility","volume":"30","author":"Morris","year":"2009","journal-title":"J Comput Chem"},{"key":"10.1124\/mol.118.113357_bib36","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/nature07923","article-title":"Detection and trapping of intermediate states priming nicotinic receptor channel opening","volume":"459","author":"Mukhtasimova","year":"2009","journal-title":"Nature"},{"key":"10.1124\/mol.118.113357_bib37","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1113\/jphysiol.1978.sp012267","article-title":"Local anaesthetics transiently block currents through single acetylcholine-receptor channels","volume":"277","author":"Neher","year":"1978","journal-title":"J Physiol"},{"key":"10.1124\/mol.118.113357_bib38","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1097\/FPC.0b013e328338ac8c","article-title":"Genetic diversity of levamisole receptor subunits in parasitic nematode species and abbreviated transcripts associated with resistance","volume":"20","author":"Neveu","year":"2010","journal-title":"Pharmacogenet Genomics"},{"key":"10.1124\/mol.118.113357_bib39","doi-asserted-by":"crossref","first-page":"8392","DOI":"10.1073\/pnas.90.18.8392","article-title":"Production of high-titer helper-free retroviruses by transient transfection","volume":"90","author":"Pear","year":"1993","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/mol.118.113357_bib40","doi-asserted-by":"crossref","unstructured":"Post MR, Tender GS, Lester HA, Dougherty DA (2017) Secondary ammonium agonists make dual cation-\u03c0 interactions in \u03b14\u03b22 nicotinic receptors. eNeuro 4(2) e0032-17.2017:1-8. DOI:http:\/\/dx.doi.org\/10.1523\/ENEURO.0032-17.2017.","DOI":"10.1523\/ENEURO.0032-17.2017"},{"key":"10.1124\/mol.118.113357_bib41","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1096\/fj.06-6264fje","article-title":"Pharmacology of N-, L-, and B-subtypes of nematode nAChR resolved at the single-channel level in Ascaris suum","volume":"20","author":"Qian","year":"2006","journal-title":"FASEB J"},{"key":"10.1124\/mol.118.113357_bib42","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/S0028-3908(01)00057-0","article-title":"The anthelmintic pyrantel acts as a low efficacious agonist and an open-channel blocker of mammalian acetylcholine receptors","volume":"41","author":"Rayes","year":"2001","journal-title":"Neuropharmacology"},{"key":"10.1124\/mol.118.113357_bib43","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1124\/mol.106.033514","article-title":"Caenorhabditis elegans muscle","volume":"71","author":"Rayes","year":"2007","journal-title":"Mol Pharmacol"},{"key":"10.1124\/mol.118.113357_bib44","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/12160","article-title":"C. elegans neuromuscular junction","volume":"2","author":"Richmond","year":"1999","journal-title":"Nat Neurosci"},{"key":"10.1124\/mol.118.113357_bib45","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1124\/jpet.102.034272","article-title":"Paraherquamide and 2-deoxy-paraherquamide distinguish cholinergic receptor subtypes in Ascaris muscle","volume":"302","author":"Robertson","year":"2002","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/mol.118.113357_bib46","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/prot.340230306","article-title":"Evaluation of comparative protein modeling by Modeller","volume":"23","author":"Sali","year":"1995","journal-title":"Proteins"},{"key":"10.1124\/mol.118.113357_bib47","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1152\/physrev.00015.2011","article-title":"End-plate acetylcholine receptor: structure, mechanism, pharmacology, and disease","volume":"92","author":"Sine","year":"2012","journal-title":"Physiol Rev"},{"key":"10.1124\/mol.118.113357_bib48","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0112102","article-title":"C. elegans larvae for gene expression profiling","volume":"9","author":"Spencer","year":"2014","journal-title":"PLoS One"},{"key":"10.1124\/mol.118.113357_bib57","doi-asserted-by":"crossref","first-page":"E2543","DOI":"10.1073\/pnas.1418289112","article-title":"Molecular blueprint of allosteric binding sites in a homologue of the agonist-binding domain of the \u03b17 nicotinic acetylcholine receptor","volume":"112","author":"Spurny","year":"2015","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/mol.118.113357_bib49","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.jmb.2004.12.031","article-title":"Refined structure of the nicotinic acetylcholine receptor at 4A resolution","volume":"346","author":"Unwin","year":"2005","journal-title":"J Mol Biol"},{"key":"10.1124\/mol.118.113357_bib50","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.jmb.2012.07.010","article-title":"Gating movement of acetylcholine receptor caught by plunge-freezing","volume":"422","author":"Unwin","year":"2012","journal-title":"J Mol Biol"},{"key":"10.1124\/mol.118.113357_bib51","doi-asserted-by":"crossref","first-page":"5539","DOI":"10.1073\/pnas.1619820114","article-title":"Brugia malayi reveal diverse muscle nAChRs and differences between cholinergic anthelmintics","volume":"114","author":"Verma","year":"2017","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/mol.118.113357_bib52","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000517","article-title":"Ascaris suum: formation of two distinct drug targets by varying the relative expression levels of two subunits","volume":"5","author":"Williamson","year":"2009","journal-title":"PLoS Pathog"},{"key":"10.1124\/mol.118.113357_bib53","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/nature07768","article-title":"Nicotine binding to brain receptors requires a strong cation-pi interaction","volume":"458","author":"Xiu","year":"2009","journal-title":"Nature"},{"key":"10.1124\/mol.118.113357_bib54","doi-asserted-by":"crossref","first-page":"611","DOI":"10.2307\/3274545","article-title":"Bephenium, a new drug active against human hookworm","volume":"44","author":"Young","year":"1958","journal-title":"J Parasitol"},{"key":"10.1124\/mol.118.113357_bib55","article-title":"C. elegans","volume":"6","author":"Zhang","year":"2011","journal-title":"PLoS One"},{"key":"10.1124\/mol.118.113357_bib56","doi-asserted-by":"crossref","unstructured":"Zhang S and Kuhn JR (2013) Cell isolation and culture. WormBook, ed. The C. elegans Research Community, WormBook, doi\/10.1895\/wormbook.1.157.1, http:\/\/www.wormbook.org.","DOI":"10.1895\/wormbook.1.157.1"}],"container-title":["Molecular Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026895X24008320?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026895X24008320?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/mol.118.113357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T10:26:48Z","timestamp":1739960808000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0026895X24008320"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":57,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["S0026895X24008320"],"URL":"https:\/\/doi.org\/10.1124\/mol.118.113357","relation":{},"ISSN":["0026-895X"],"issn-type":[{"value":"0026-895X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Activation of Caenorhabditis elegans Levamisole-Sensitive and Mammalian Nicotinic Receptors by the Antiparasitic Bephenium","name":"articletitle","label":"Article Title"},{"value":"Molecular Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/mol.118.113357","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 by The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}