{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:09:35Z","timestamp":1760299775304,"version":"3.37.3"},"reference-count":39,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[1998,12,1]],"date-time":"1998-12-01T00:00:00Z","timestamp":912470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[1998,12,1]],"date-time":"1998-12-01T00:00:00Z","timestamp":912470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["molpharm.aspetjournals.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular Pharmacology"],"published-print":{"date-parts":[[1998,12]]},"DOI":"10.1124\/mol.54.6.968","type":"journal-article","created":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T20:32:14Z","timestamp":1515443534000},"page":"968-978","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":33,"title":["A Hydrophobic Cluster between Transmembrane Helices 5 and 6 Constrains the Thyrotropin-Releasing Hormone Receptor in an Inactive Conformation"],"prefix":"10.1016","volume":"54","author":[{"given":"Anny-Odile","family":"Colson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey H.","family":"Perlman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arti","family":"Jinsi-Parimoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel R.","family":"Nussenzveig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Osman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marvin C.","family":"Gershengorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1124\/mol.54.6.968_bib1","doi-asserted-by":"crossref","first-page":"12470","DOI":"10.1021\/bi960849l","article-title":"Structural features and light dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site directed spin labeling study.","volume":"35","author":"Altenbach","year":"1996","journal-title":"Biochemistry"},{"key":"10.1124\/mol.54.6.968_bib2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S1043-2760(98)00007-1","article-title":"Constitutively signaling G protein-coupled receptors and human disease.","volume":"9","author":"Arvanitakis","year":"1998","journal-title":"Trends Endocrinol Metab"},{"key":"10.1124\/mol.54.6.968_bib3","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/0955-0674(94)90134-1","article-title":"Structure and function of receptors coupled to G proteins.","volume":"6","author":"Baldwin","year":"1994","journal-title":"Curr Opin Cell Biol"},{"key":"10.1124\/mol.54.6.968_bib4","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0006-3495(92)81794-0","article-title":"Analysis and refinement of criteria for predicting structure and relative orientations of transmembranal helical domains.","volume":"62","author":"Ballesteros","year":"1992","journal-title":"Biophys J"},{"key":"10.1124\/mol.54.6.968_bib5","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/jcc.540040211","article-title":"CHARMM: a program for macromolecular energy minimization and dynamics calculations.","volume":"4","author":"Brooks","year":"1983","journal-title":"J Comp Chem"},{"key":"10.1124\/mol.54.6.968_bib6","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1210\/endo.138.4.5046","article-title":"Human calcitonin receptors exhibit agonist-independent (constitutive) signaling activity.","volume":"138","author":"Cohen","year":"1997","journal-title":"Endocrinology"},{"key":"10.1124\/mol.54.6.968_bib7","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/S0006-3495(98)77827-0","article-title":"Static and dynamic roles of extracellular loops in G-protein-coupled receptors: a mechanism for sequential binding of thyrotropin-releasing hormone to its receptor.","volume":"74","author":"Colson","year":"1998","journal-title":"Biophys J"},{"key":"10.1124\/mol.54.6.968_bib8","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1126\/science.274.5288.768","article-title":"Requirement of rigid body motion of transmembrane helices for light activation of rhodopsin.","volume":"274","author":"Farrens","year":"1996","journal-title":"Science (Washington DC)"},{"key":"10.1124\/mol.54.6.968_bib9","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1152\/physrev.1996.76.1.175","article-title":"Molecular and cellular biology of thyrotropin-releasing hormone (TRH) receptors.","volume":"76","author":"Gershengorn","year":"1996","journal-title":"Physiol Rev"},{"key":"10.1124\/mol.54.6.968_bib10","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1074\/jbc.272.5.2587","article-title":"Structural instability of a constitutively active G protein-coupled receptor: agonist-independent activation due to conformational flexibility.","volume":"272","author":"Gether","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib11","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1074\/jbc.272.3.1822","article-title":"Mutation of Asn111 in the third transmembrane domain of the AT1A angiotensin II receptor induces its constitutive activation.","volume":"272","author":"Groblewski","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib12","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1021\/bi972241y","article-title":"A cluster of aromatic residues in the sixth membrane-spanning segment of the dopamine D2 receptor is accessible in the binding-site crevice.","volume":"37","author":"Javitch","year":"1998","journal-title":"Biochemistry"},{"key":"10.1124\/mol.54.6.968_bib13","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1210\/endo.138.4.5059","article-title":"Constitutive activity of native thyrotropin-releasing hormone receptors revealed using a protein kinase C-responsive reporter gene.","volume":"138","author":"Jinsi-Parimoo","year":"1997","journal-title":"Endocrinology"},{"key":"10.1124\/mol.54.6.968_bib14","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1016\/S0021-9258(18)45962-5","article-title":"Constitutive activation of the \u03b11B-adrenergic receptor by all amino acid substitutions at a single site: evidence for a region which constrains receptor activation.","volume":"267","author":"Kjelsberg","year":"1992","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib15","doi-asserted-by":"crossref","first-page":"6764","DOI":"10.1073\/pnas.93.13.6764","article-title":"Mutation of Pro-258 in transmembrane domain 6 constitutively activates the G protein-coupled \u03b1-factor receptor.","volume":"93","author":"Konopka","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1124\/mol.54.6.968_bib16","doi-asserted-by":"crossref","first-page":"7651","DOI":"10.1021\/bi952203j","article-title":"A refined model of the thyrotropin-releasing hormone (TRH) binding pocket: novel mixed mode Monte Carlo\/stochastic dynamics simulations of the complex between TRH and TRH receptor.","volume":"35","author":"Laakkonen","year":"1996","journal-title":"Biochemistry"},{"key":"10.1124\/mol.54.6.968_bib17","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0165-6147(93)90048-O","article-title":"Constitutive activity of receptors coupled to guanine nucleotide regulatory proteins.","volume":"14","author":"Lefkowitz","year":"1993","journal-title":"Trends Pharmacol Sci"},{"key":"10.1124\/mol.54.6.968_bib18","doi-asserted-by":"crossref","first-page":"11149","DOI":"10.1021\/bi960858u","article-title":"Specific tryptophan UV-absorbance changes are probes of the transition of rhodopsin to its active state.","volume":"35","author":"Lin","year":"1996","journal-title":"Biochemistry"},{"key":"10.1124\/mol.54.6.968_bib19","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1093\/protein\/10.5.501","article-title":"A model of the lutropin\/choriogonadotropin receptor: insights into the structural and functional effects of constitutively activating mutations.","volume":"10","author":"Lin","year":"1997","journal-title":"Protein Eng"},{"key":"10.1124\/mol.54.6.968_bib20","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1074\/jbc.270.3.1041","article-title":"Truncation of the thyrotropin-releasing hormone carboxyl tail causes constitutive activity and leads to impaired responsiveness in Xenopus oocytes and AtT-20 cells.","volume":"270","author":"Matus-Leibovitch","year":"1995","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib21","doi-asserted-by":"crossref","first-page":"4269","DOI":"10.1016\/S0021-9258(20)64317-4","article-title":"Mapping of the amino acids in membrane-embedded helices that interact with the retinal chromophore in bovine rhodopsin.","volume":"266","author":"Nakayama","year":"1991","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib22","first-page":"1105","article-title":"Decreased levels of internalized thyrotropin-releasing hormone receptors after uncoupling from guanine nucleotide-binding protein and phospholipase-C.","volume":"7","author":"Nussenzveig","year":"1993","journal-title":"Mol Endocrinol"},{"key":"10.1124\/mol.54.6.968_bib23","doi-asserted-by":"crossref","first-page":"28123","DOI":"10.1016\/S0021-9258(18)46903-7","article-title":"Inhibition of inositol phosphate second messenger formation by intracellular loop 1 of a human calcitonin receptor: expression and mutational analysis of synthetic receptor genes.","volume":"269","author":"Nussenzveig","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib24","doi-asserted-by":"crossref","first-page":"11937","DOI":"10.1074\/jbc.272.18.11937","article-title":"Interactions between conserved residues in transmembrane helices 1, 2 and 7 of the thyrotropin-releasing hormone receptor.","volume":"272","author":"Perlman","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib25","doi-asserted-by":"crossref","first-page":"7643","DOI":"10.1021\/bi952202r","article-title":"A refined model of the thyrotropin-releasing hormone (TRH) receptor binding pocket: experimental analysis and energy minimization of the complex between TRH and TRH receptor.","volume":"35","author":"Perlman","year":"1996","journal-title":"Biochemistry"},{"key":"10.1124\/mol.54.6.968_bib26","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/S0026-895X(25)08568-2","article-title":"Distinct roles for arginines in transmembrane helices 6 and 7 of the thyrotropin-releasing hormone receptor.","volume":"47","author":"Perlman","year":"1995","journal-title":"Mol Pharmacol"},{"key":"10.1124\/mol.54.6.968_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/dna.1992.11.1","article-title":"Sequence alignment of the G-protein coupled receptor superfamily.","volume":"11","author":"Probst","year":"1992","journal-title":"DNA Cell Biol"},{"key":"10.1124\/mol.54.6.968_bib28","doi-asserted-by":"crossref","first-page":"13368","DOI":"10.1021\/bi961163w","article-title":"Na+ ions binding to the bradykinin B2 receptor suppress agonist-independent receptor activation.","volume":"35","author":"Quitterer","year":"1996","journal-title":"Biochemistry"},{"key":"10.1124\/mol.54.6.968_bib29","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/0896-6273(92)90034-B","article-title":"Constitutively active mutants of rhodopsin.","volume":"9","author":"Robinson","year":"1992","journal-title":"Neuron"},{"key":"10.1124\/mol.54.6.968_bib30","doi-asserted-by":"crossref","first-page":"4625","DOI":"10.1016\/S0021-9258(18)53442-6","article-title":"A mutation-induced activated state of the \u03b22-adrenergic receptor: extending the ternary complex model.","volume":"268","author":"Samama","year":"1993","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib31","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1111\/j.1399-3011.1992.tb01595.x","article-title":"Geometry of proline-containing alpha-helices in proteins.","volume":"39","author":"Sankararamakrishnan","year":"1992","journal-title":"Int J Peptide Protein Res"},{"key":"10.1124\/mol.54.6.968_bib32","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1091\/mbc.3.8.941","article-title":"Regulation of gene expression in PC-12 cells via an activator of dual second messengers: pituitary adenylate cyclase-activating polypeptide.","volume":"3","author":"Schadlow","year":"1992","journal-title":"Mol Biol Cell"},{"key":"10.1124\/mol.54.6.968_bib33","doi-asserted-by":"crossref","first-page":"3566","DOI":"10.1002\/j.1460-2075.1996.tb00726.x","article-title":"Constitutively active mutants of the \u03b11B-adrenergic receptor: role of highly conserved polar amino acids in receptor activation.","volume":"15","author":"Scheer","year":"1996","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"10.1124\/mol.54.6.968_bib34","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1016\/S0022-3565(25)12181-2","article-title":"Pharmacology of a constitutively active muscarinic receptor generated by random mutagenesis.","volume":"275","author":"Spalding","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"10.1124\/mol.54.6.968_bib35","doi-asserted-by":"crossref","first-page":"27925","DOI":"10.1016\/S0021-9258(18)46876-7","article-title":"High agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype.","volume":"269","author":"Tiberi","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1124\/mol.54.6.968_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1098-2299(199601)37:1<1::AID-DDR1>3.0.CO;2-S","article-title":"Molecular architecture of G protein-coupled receptors.","volume":"37","author":"Van Rhee","year":"1996","journal-title":"Drug Dev Res"},{"key":"10.1124\/mol.54.6.968_bib37","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/j.1460-2075.1993.tb05661.x","article-title":"Functional role of proline and tryptophan residues highly conserved among G protein-coupled receptors studied by mutational analysis of the m3 muscarinic receptor.","volume":"12","author":"Wess","year":"1993","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"10.1124\/mol.54.6.968_bib38","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1016\/S0006-3495(97)78267-5","article-title":"Molecular dynamics of individual alpha-helices of bacteriorhodopsin in dimyristoyl phosphatidylcholine. I. Structure and dynamics.","volume":"73","author":"Woolf","year":"1997","journal-title":"Biophys J"},{"key":"10.1124\/mol.54.6.968_bib39","doi-asserted-by":"crossref","first-page":"14024","DOI":"10.1074\/jbc.270.23.14024","article-title":"Mutagenesis and the molecular modeling of the rat angiotensin II receptor (AT1).","volume":"270","author":"Yamano","year":"1995","journal-title":"J Biol Chem"}],"container-title":["Molecular Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026895X24130179?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026895X24130179?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1124\/mol.54.6.968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T09:56:02Z","timestamp":1740045362000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0026895X24130179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,12]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,12]]}},"alternative-id":["S0026895X24130179"],"URL":"https:\/\/doi.org\/10.1124\/mol.54.6.968","relation":{},"ISSN":["0026-895X"],"issn-type":[{"type":"print","value":"0026-895X"}],"subject":[],"published":{"date-parts":[[1998,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Hydrophobic Cluster between Transmembrane Helices 5 and 6 Constrains the Thyrotropin-Releasing Hormone Receptor in an Inactive Conformation","name":"articletitle","label":"Article Title"},{"value":"Molecular Pharmacology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1124\/mol.54.6.968","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 1998 The American Society for Pharmacology and Experimental Therapeutics","name":"copyright","label":"Copyright"}]}}