{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:18:22Z","timestamp":1778822302586,"version":"3.51.4"},"reference-count":16,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5857","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2007,12,14]]},"abstract":"<jats:p>\n                    RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr\n                    <jats:sup>1<\/jats:sup>\n                    -Ser\n                    <jats:sup>2<\/jats:sup>\n                    -Pro\n                    <jats:sup>3<\/jats:sup>\n                    -Thr\n                    <jats:sup>4<\/jats:sup>\n                    -Ser\n                    <jats:sup>5<\/jats:sup>\n                    -Pro\n                    <jats:sup>6<\/jats:sup>\n                    -Ser\n                    <jats:sup>7<\/jats:sup>\n                    . Differential phosphorylation of serine-2 and serine-5 at the 5\u2032 and 3\u2032 regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes. This position does not appear to be phosphorylated in CTDs of less than 20 consensus repeats. The position of repeats where serine-7 is substituted influenced the appearance of distinct phosphorylated forms, suggesting functional differences between CTD regions. Our results indicate that restriction of serine-7 epitopes to the Linker-proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression.\n                  <\/jats:p>","DOI":"10.1126\/science.1145977","type":"journal-article","created":{"date-parts":[[2007,12,13]],"date-time":"2007-12-13T16:43:19Z","timestamp":1197564199000},"page":"1780-1782","source":"Crossref","is-referenced-by-count":271,"title":["Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7"],"prefix":"10.1126","volume":"318","author":[{"given":"Rob D.","family":"Chapman","sequence":"first","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Heidemann","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas K.","family":"Albert","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Mailhammer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Flatley","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Meisterernst","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Kremmer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Eick","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany."},{"name":"Institute of Molecular Immunology, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science, Marchioninistrasse 25, 81377 Munich, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1318105"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1477006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03794.x"},{"key":"e_1_3_2_5_2","first-page":"405","volume":"35","year":"1992","unstructured":"E. 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We thank A. Meinhart and P. Cramer for critical comments on this manuscript."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1145977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T02:50:19Z","timestamp":1704855019000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1145977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12,14]]},"references-count":16,"journal-issue":{"issue":"5857","published-print":{"date-parts":[[2007,12,14]]}},"alternative-id":["10.1126\/science.1145977"],"URL":"https:\/\/doi.org\/10.1126\/science.1145977","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1097491.553566","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,12,14]]}}}