{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T09:21:21Z","timestamp":1778404881206,"version":"3.51.4"},"reference-count":25,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5867","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2008,2,29]]},"abstract":"<jats:p>Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation.<\/jats:p>","DOI":"10.1126\/science.1153124","type":"journal-article","created":{"date-parts":[[2008,2,28]],"date-time":"2008-02-28T17:20:02Z","timestamp":1204219202000},"page":"1244-1247","source":"Crossref","is-referenced-by-count":3131,"title":["Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes"],"prefix":"10.1126","volume":"319","author":[{"given":"Katarina","family":"Trajkovic","sequence":"first","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Chieh","family":"Hsu","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Salvatore","family":"Chiantia","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Lawrence","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Dirk","family":"Wenzel","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Felix","family":"Wieland","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Petra","family":"Schwille","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Britta","family":"Bru\u0308gger","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]},{"given":"Mikael","family":"Simons","sequence":"additional","affiliation":[{"name":"Centre for Biochemistry and Molecular Cell Biology, University of Go\u0308ttingen, 37073 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Experimental Medicine, 37075 Go\u0308ttingen, Germany."},{"name":"Max-Planck-Institute for Biophysical Chemistry, 37077 Go\u0308ttingen, Germany."},{"name":"Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany."},{"name":"BioTec, TU Dresden, 01307 Dresden, Germany."}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.23.090506.123319"},{"key":"e_1_3_2_3_2","first-page":"317","volume":"5","year":"2004","unstructured":"J. Gruenberg, H. Stenmark, Nat. Rev. Mol. Cell Biol.5, 317 (2004).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri855"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.30502.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvj128"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200509022"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-03-0871"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308413101"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06381.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb791"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02978"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.35.040405.102126"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2162"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00506-2"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.11.6507-6519.2003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207550200"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511136103"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi061307z"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76640-9"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.1.39"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.30.21121"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207146"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092425"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.08.003"},{"key":"e_1_3_2_26_2","unstructured":"We are grateful to R. Bittman D. Caplan M. Zerial D. Arndt-Jovin U. Coskun P. Keller D. Cutler P. Burfeind and H. Stenmark for providing reagents and R. White for help with RNAi experiments. The work was supported by the Deutsche Forschungsgemeinschaft (SFB 523 GRK521)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.1153124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T04:45:48Z","timestamp":1704861948000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.1153124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2,29]]},"references-count":25,"journal-issue":{"issue":"5867","published-print":{"date-parts":[[2008,2,29]]}},"alternative-id":["10.1126\/science.1153124"],"URL":"https:\/\/doi.org\/10.1126\/science.1153124","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1103213.12436061","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1103213.560533","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1103213.559283","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1103213.559210","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1103213.560921","asserted-by":"object"}]},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2,29]]}}}