{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T04:11:05Z","timestamp":1776831065544,"version":"3.51.2"},"reference-count":31,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5891","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2008,8,15]]},"abstract":"<jats:p>The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and 34% to nonannotated genomic regions. On the basis of known transcripts, RNA-Seq can detect 25% more genes than can microarrays. A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing.<\/jats:p>","DOI":"10.1126\/science.1160342","type":"journal-article","created":{"date-parts":[[2008,7,3]],"date-time":"2008-07-03T21:10:22Z","timestamp":1215119422000},"page":"956-960","source":"Crossref","is-referenced-by-count":1049,"title":["A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome"],"prefix":"10.1126","volume":"321","author":[{"given":"Marc","family":"Sultan","sequence":"first","affiliation":[{"name":"Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany."},{"name":"Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany."},{"name":"International Max Planck Research School for 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