{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:54:46Z","timestamp":1778288086916,"version":"3.51.4"},"reference-count":28,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5940","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2009,7,31]]},"abstract":"<jats:title>Gradual Unpacking<\/jats:title>\n                  <jats:p>\n                    Eukaryotic DNA is packaged onto nucleosomes, which form the main constituent of chromatin. This packaging material presents a barrier to accessing the genome by the various machineries that need to deal with the DNA: replication, recombination, repair, and transcription complexes, for example.\n                    <jats:bold>\n                      Hodges\n                      <jats:italic>et al.<\/jats:italic>\n                    <\/jats:bold>\n                    (p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" page=\"626\" related-article-type=\"in-this-issue\" vol=\"325\" xlink:href=\"10.1126\/science.1172926\">626<\/jats:related-article>\n                    ; see the Perspective by\n                    <jats:bold>\n                      <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"5940\" page=\"547\" related-article-type=\"in-this-issue\" vol=\"325\" xlink:href=\"10.1126\/science.1177311\">Otterstrom and van Oijen<\/jats:related-article>\n                    <\/jats:bold>\n                    ) use single-molecule techniques to analyze how a yeast RNA polymerase II ternary elongation complex copes when it encounters a single nucleosome directly in its path. The polymerase does not actively peel the DNA from the nucleosome's surface but, instead, waits patiently until the DNA fluctuates off the nucleosome and then advances, increment by increment, until the nucleosome is destabilized. Under certain conditions the destabilized nucleosome, rather than being lost entirely from the DNA, can be passed back to the DNA behind the polymerase.\n                  <\/jats:p>","DOI":"10.1126\/science.1172926","type":"journal-article","created":{"date-parts":[[2009,7,30]],"date-time":"2009-07-30T18:31:18Z","timestamp":1248978678000},"page":"626-628","source":"Crossref","is-referenced-by-count":334,"title":["Nucleosomal Fluctuations Govern the Transcription Dynamics of RNA Polymerase II"],"prefix":"10.1126","volume":"325","author":[{"given":"Courtney","family":"Hodges","sequence":"first","affiliation":[{"name":"Jason L. Choy Laboratory of Single-Molecule Biophysics and Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lacramioara","family":"Bintu","sequence":"additional","affiliation":[{"name":"Department of Physics, University of California, Berkeley, CA 94720, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucyna","family":"Lubkowska","sequence":"additional","affiliation":[{"name":"NCI Center for Cancer Research, National Cancer Institute\u2013Frederick Cancer Research and Development Center, Frederick, MD 21702, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikhail","family":"Kashlev","sequence":"additional","affiliation":[{"name":"NCI Center for Cancer Research, National Cancer Institute\u2013Frederick Cancer Research and Development Center, Frederick, MD 21702, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Bustamante","sequence":"additional","affiliation":[{"name":"Jason L. 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