{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T23:46:14Z","timestamp":1771976774030,"version":"3.50.1"},"reference-count":37,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5203","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1995,3,10]]},"abstract":"<jats:p>\n            The yeast retroviruslike element Ty3 inserts at the transcription initiation sites of genes transcribed by RNA polymerase III (Pol III). An in vitro integration assay was developed with the use of Ty3 viruslike particles and a modified\n            <jats:italic>SUP2<\/jats:italic>\n            tyrosine transfer RNA (tRNA\n            <jats:sup>Tyr<\/jats:sup>\n            ) gene target. Integration was position-specific and required Ty3 integrase, Pol III transcription extract, and a transcriptionally competent tRNA gene. Use of individual transcription factor (TF) IIIB-, TFIIIC-, and Pol III-containing fractions showed that TFIIIB and TFIIIC, together, were sufficient for position-specific Ty3 integration, but not for transcription. This report demonstrates that in vitro integration of a retroelement can be targeted by cellular proteins.\n          <\/jats:p>","DOI":"10.1126\/science.7878467","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T18:19:42Z","timestamp":1161973182000},"page":"1488-1491","source":"Crossref","is-referenced-by-count":160,"title":["Requirement of RNA Polymerase III Transcription Factors for in Vitro Position-Specific Integration of a Retroviruslike Element"],"prefix":"10.1126","volume":"267","author":[{"given":"Jacqueline","family":"Kirchner","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92717, USA."}]},{"given":"Charles M.","family":"Connolly","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92717, USA."}]},{"given":"Suzanne B.","family":"Sandmeyer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92717, USA."}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/0092-8674(83)90398-7","volume":"34","year":"1983","unstructured":"ALLISON, D.S., THE PROMOTER SEQUENCE OF A YEAST TRANSFER RNATYR GENE, CELL 34: 655 (1983).","journal-title":"CELL"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"5275","DOI":"10.1016\/S0021-9258(19)57210-6","volume":"261","year":"1986","unstructured":"BAKER, R.E., EFFECTS OF TRANSFER-RNA TYR POINT MUTATIONS ON THE BINDING OF YEAST RNA POLYMERASE-III TRANSCRIPTION FACTOR-C, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 5275 (1986).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"4768","DOI":"10.1128\/jvi.68.8.4768-4775.1994","volume":"68","year":"1994","unstructured":"CANNON, P.M., HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE - EFFECT ON VIRAL REPLICATION OF MUTATIONS AT HIGHLY CONSERVED RESIDUES, JOURNAL OF VIROLOGY 68: 4768 (1994).","journal-title":"JOURNAL OF VIROLOGY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1101\/gad.6.1.117","volume":"6","year":"1992","unstructured":"CHALKER, D.L., TY3 INTEGRATES WITHIN THE REGION OF RNA POLYMERASE-III TRANSCRIPTION INITIATION, GENES & DEVELOPMENT 6: 117 (1992).","journal-title":"GENES & DEVELOPMENT"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1093\/genetics\/126.4.837","volume":"126","year":"1990","unstructured":"CHALKER, D.L., TRANSFER-RNA GENES ARE GENOMIC TARGETS FOR DENOVO TRANSPOSITION OF THE YEAST RETROTRANSPOSON TY3, GENETICS 126: 837 (1990).","journal-title":"GENETICS"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0168-9525(92)90223-Q","volume":"8","year":"1992","unstructured":"CRAIGIE, R, HOTSPOTS AND WARM SPOTS - INTEGRATION SPECIFICITY OF RETROELEMENTS, TRENDS IN GENETICS 8: 187 (1992).","journal-title":"TRENDS IN GENETICS"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/0042-6822(92)90499-F","volume":"188","year":"1992","unstructured":"DRELICH, M, IDENTIFICATION OF AMINO-ACID-RESIDUES CRITICAL FOR ENDONUCLEASE AND INTEGRATION ACTIVITIES OF HIV-1 IN PROTEIN INVITRO, VIROLOGY 188: 459 (1992).","journal-title":"VIROLOGY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1038\/307386a0","volume":"307","year":"1984","unstructured":"EIBEL, H, PREFERENTIAL INTEGRATION OF YEAST TRANSPOSABLE ELEMENT TY INTO A PROMOTER REGION, NATURE 307: 386 (1984).","journal-title":"NATURE"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"6361","DOI":"10.1128\/jvi.66.11.6361-6369.1992","volume":"66","year":"1992","unstructured":"ENGLEMAN, A, J VIROL 66: 6361 (1992).","journal-title":"J VIROL"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1146\/annurev.bi.57.070188.004301","volume":"57","year":"1988","unstructured":"GEIDUSCHEK, E.P., TRANSCRIPTION BY RNA POLYMERASE-III, ANNUAL REVIEW OF BIOCHEMISTRY 57: 873 (1988).","journal-title":"ANNUAL REVIEW OF BIOCHEMISTRY"},{"key":"e_1_2_1_11_1","first-page":"CH5","year":"1994","unstructured":"Geiduschek, E. 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