{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T17:32:30Z","timestamp":1776101550210,"version":"3.50.1"},"reference-count":30,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"92","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Robot."],"published-print":{"date-parts":[[2024,7,31]]},"abstract":"<jats:p>\n            Effective mosquito population suppression has been repeatedly demonstrated in field trials through the release of male mosquitoes to induce sterile mating with wild females using the incompatible insect technique (IIT), the sterile insect technique (SIT), or their combination. However, upscaling these techniques requires a highly efficient and scalable approach for the sex separation of mass-reared mosquitoes to minimize the unintentional release of females, which can lead to either population replacement or biting nuisance, a major bottleneck up to now. Here, we report the successful development of an automated mosquito pupa sex sorter that can effectively separate large numbers of males from females for population suppression of\n            <jats:italic>Aedes aegypti<\/jats:italic>\n            ,\n            <jats:italic>A. albopictus<\/jats:italic>\n            , and\n            <jats:italic>Culex quinquefasciatus<\/jats:italic>\n            . The male production capacity of the automated sex sorter was increased by ~17-fold compared with manual sex separation with the Fay-Morlan sorter and enabled one person to separate 16 million males per week. With ~0.5% female contamination, the produced males exhibited high flight ability and mating performance. The field trial demonstrates that the quality of\n            <jats:italic>A. albopictus<\/jats:italic>\n            males produced using the automated sex sorter is suitable for inducing population suppression. These results indicate that the automated sex sorter offers the potential to upscale IIT and SIT against mosquito vectors for disease control.\n          <\/jats:p>","DOI":"10.1126\/scirobotics.adj6261","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T18:11:43Z","timestamp":1722449503000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":17,"title":["Upscaling the production of sterile male mosquitoes with an automated pupa sex sorter"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6120-1688","authenticated-orcid":true,"given":"Jun-Tao","family":"Gong","sequence":"first","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5455-9772","authenticated-orcid":true,"given":"Wadaka","family":"Mamai","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."},{"name":"Institut de Recherche Agricole pour le D\u00e9veloppement (IRAD), Yaound\u00e9, Cameroun."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qixian","family":"Tang","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanhui","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jixin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8662-4700","authenticated-orcid":true,"given":"Hamidou","family":"Maiga","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."},{"name":"Institut de Recherche en Sciences de la Sant\u00e9, Direction R\u00e9gionale de l\u2019Ouest (IRSS-DRO), Bobo-Dioulasso, Burkina Faso."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanwintoum S\u00e9verin Bimbil\u00e9","family":"Somda","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."},{"name":"Unit\u00e9 de Formation et de Recherche en Science et Technologie (UFR\/ST), Universit\u00e9 Norbert ZONGO (UNZ), Koudougou, Burkina Faso."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Martina","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9360-066X","authenticated-orcid":true,"given":"Simran Singh","family":"Kotla","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4060-2221","authenticated-orcid":true,"given":"Thomas","family":"Wallner","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1913-416X","authenticated-orcid":true,"given":"J\u00e9r\u00e9my","family":"Bouyer","sequence":"additional","affiliation":[{"name":"Insect Pest Control Sub-programme, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria."},{"name":"ASTRE, CIRAD, Montpellier, France."},{"name":"ASTRE, CIRAD, INRAE, University of Montpellier, Plateforme Technologique CYROI, Sainte-Clotilde, La R\u00e9union, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-012X","authenticated-orcid":true,"given":"Zhiyong","family":"Xi","sequence":"additional","affiliation":[{"name":"Guangzhou Wolbaki Biotech Co. Ltd., Guangzhou, China."},{"name":"Department of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, MI, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","unstructured":"Global Vector Control Response 2017\u20132030 (World Health Organization 2017)."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2968"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1407-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0471-x"},{"key":"e_1_3_2_6_2","unstructured":"R. S. Lees D. O. Carvalho J. Bouyer Principles and practice in area-wide integrated pest management in Sterile Insect Technique A. V. Dyck J. Hendrichs A. S. Robinson Eds. 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M. Hamidou Maiga H. Yamada R. Argil\u00e9s Herrero J. Bouyer \"Guidelines for mass-rearing of Aedes mosquitoes\" (FAO\/IAEA 2020) pp. 24."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/insects11110801"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007775"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/iead060"}],"container-title":["Science Robotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/scirobotics.adj6261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T18:12:03Z","timestamp":1722449523000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/scirobotics.adj6261"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,31]]},"references-count":30,"journal-issue":{"issue":"92","published-print":{"date-parts":[[2024,7,31]]}},"alternative-id":["10.1126\/scirobotics.adj6261"],"URL":"https:\/\/doi.org\/10.1126\/scirobotics.adj6261","relation":{},"ISSN":["2470-9476"],"issn-type":[{"value":"2470-9476","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,31]]},"assertion":[{"value":"2023-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadj6261"}}