{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T19:12:44Z","timestamp":1792177964584,"version":"4.4.0"},"reference-count":40,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"8","funder":[{"name":"NIAID\/NIH","award":["AI111538"],"award-info":[{"award-number":["AI111538"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2019,8,2]]},"abstract":"<jats:p>An engineered viral vector of bacteriophage T4 linked to AAV delivers large payloads of genes and proteins into human cells.<\/jats:p>","DOI":"10.1126\/sciadv.aax0064","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T23:41:39Z","timestamp":1566430899000},"source":"Crossref","is-referenced-by-count":49,"title":["A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells"],"prefix":"10.1126","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-7053","authenticated-orcid":true,"given":"Jingen","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Biology, The Catholic University of America, Washington, DC 20064, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Biology, The Catholic University of America, Washington, DC 20064, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marthandan","family":"Mahalingam","sequence":"additional","affiliation":[{"name":"Department of Biology, The Catholic University of America, Washington, DC 20064, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Sha","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Kilgore","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok K.","family":"Chopra","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0777-6587","authenticated-orcid":true,"given":"Venigalla","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Biology, The Catholic University of America, Washington, DC 20064, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3499"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19764"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.jconrel.2012.04.008","article-title":"Virus-mediated gene delivery for human gene therapy","volume":"161","author":"Giacca M.","year":"2012","unstructured":"M. Giacca, S. Zacchigna, Virus-mediated gene delivery for human gene therapy. J. Control. Release 161, 377\u2013388 (2012).","journal-title":"J. Control. Release"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1021\/acs.accounts.5b00557","article-title":"Phage as a genetically modifiable supramacromolecule in chemistry, materials and medicine","volume":"49","author":"Cao B.","year":"2016","unstructured":"B. Cao, M. Yang, C. Mao, Phage as a genetically modifiable supramacromolecule in chemistry, materials and medicine. Acc. Chem. Res. 49, 1111\u20131120 (2016).","journal-title":"Acc. Chem. Res."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2016.03.003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"e1000592","DOI":"10.1371\/journal.pbio.1000592","article-title":"A promiscuous DNA packaging machine from bacteriophage T4","volume":"9","author":"Zhang Z.","year":"2011","unstructured":"Z. Zhang, V. I. Kottadiel, R. Vafabakhsh, L. Dai, Y. R. Chemla, T. Ha, V. B. Rao, A promiscuous DNA packaging machine from bacteriophage T4. PLOS Biol. 9, e1000592 (2011).","journal-title":"PLOS Biol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1006\/jmbi.1996.0291","article-title":"A discontinuous headful packaging model for packaging less than headful length DNA molecules by bacteriophage T4","volume":"258","author":"Leffers G.","year":"1996","unstructured":"G. Leffers, V. B. Rao, A discontinuous headful packaging model for packaging less than headful length DNA molecules by bacteriophage T4. J. Mol. Biol. 258, 839\u2013850 (1996).","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.05.008"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.2874-2878.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300867110"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01926-18"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003495"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aar4134"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.7b00179"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-031413-085355"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1708483114"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00847-11"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.1438-1445.1998"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"17148","DOI":"10.1073\/pnas.0508477102","article-title":"Parvoviral virions deploy a capsid-tethered lipolytic enzyme to breach the endosomal membrane during cell entry","volume":"102","author":"Farr G. A.","year":"2005","unstructured":"G. A. Farr, L. G. Zhang, P. Tattersall, Parvoviral virions deploy a capsid-tethered lipolytic enzyme to breach the endosomal membrane during cell entry. Proc. Natl. Acad. Sci. U.S.A. 102, 17148\u201317153 (2005).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"e_1_3_2_21_2","first-page":"1138","article-title":"Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: A 13 residue consensus peptide specifies biotinylation in Escherichia coli","volume":"11","author":"Schatz P. J.","year":"1993","unstructured":"P. J. Schatz, Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: A 13 residue consensus peptide specifies biotinylation in Escherichia coli. Biotechnology 11, 1138\u20131143 (1993).","journal-title":"Biotechnology"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00254-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ymthe.2006.02.014","article-title":"Metabolic biotinylation provides a unique platform for the purification and targeting of multiple AAV vector serotypes","volume":"14","author":"Arnold G. S.","year":"2006","unstructured":"G. S. Arnold, A. K. Sasser, M. D. Stachler, J. S. Bartlett, Metabolic biotinylation provides a unique platform for the purification and targeting of multiple AAV vector serotypes. Mol. Ther. 14, 97\u2013106 (2006).","journal-title":"Mol. Ther."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-100114-055212"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb12956.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S1534-5807(01)00031-4","article-title":"A viral phospholipase A2 is required for parvovirus infectivity","volume":"1","author":"Zadori Z.","year":"2001","unstructured":"Z. Zadori, J. Szelei, M. C. Lacoste, Y. Li, S. Gariepy, P. Raymond, M. Allaire, I. R. Nabi, P. Tijssen, A viral phospholipase A2 is required for parvovirus infectivity. Dev. Cell 1, 291\u2013302 (2001).","journal-title":"Dev. Cell"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"7875","DOI":"10.1128\/JVI.00649-08","article-title":"Adeno-associated virus vector genomes persist as episomal chromatin in primate muscle","volume":"82","author":"Penaud-Budloo M.","year":"2008","unstructured":"M. Penaud-Budloo, C. Le Guiner, A. Nowrouzi, A. Toromanoff, Y. Cherel, P. Chenuaud, M. Schmidt, C. von Kalle, F. Rolling, P. Moullier, R. O. Snyder, Adeno-associated virus vector genomes persist as episomal chromatin in primate muscle. J. Virol. 82, 7875\u20137885 (2008).","journal-title":"J. Virol."},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1021\/acsnano.6b07343","article-title":"Immunological principles guiding the rational design of particles for vaccine delivery","volume":"11","author":"Gause K. T.","year":"2017","unstructured":"K. T. Gause, A. K. Wheatley, J. Cui, Y. Yan, S. J. Kent, F. Caruso, Immunological principles guiding the rational design of particles for vaccine delivery. ACS Nano 11, 54\u201368 (2017).","journal-title":"ACS Nano"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305923110"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.immuni.2010.10.004","article-title":"Immunologic basis of vaccine vectors","volume":"33","author":"Liu M. A.","year":"2010","unstructured":"M. A. Liu, Immunologic basis of vaccine vectors. Immunity 33, 504\u2013515 (2010).","journal-title":"Immunity"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1089\/hum.2018.29073.trf","article-title":"DNA vaccination in 2018: An update","volume":"29","author":"Flotte T. R.","year":"2018","unstructured":"T. R. Flotte, S. Lu, DNA vaccination in 2018: An update. Hum. Gene Ther. 29, 963\u2013965 (2018).","journal-title":"Hum. Gene Ther."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac7263"},{"key":"e_1_3_2_33_2","article-title":"Bacteriophage T4 nanoparticles for vaccine delivery against infectious diseases","author":"Tao P.","year":"2018","unstructured":"P. Tao, J. Zhu, M. Mahalingam, H. Batra, V. B. Rao, Bacteriophage T4 nanoparticles for vaccine delivery against infectious diseases. Adv. Drug Deliv. Rev. (2018).","journal-title":"Adv. Drug Deliv. Rev."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.11.1545"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1520112113"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201200446"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1112"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1002\/eji.200323919","article-title":"Role of Toll-like receptors in costimulating cytotoxic T cell responses","volume":"33","author":"Schwarz K.","year":"2003","unstructured":"K. Schwarz, T. Storni, V. Manolova, A. Didierlaurent, J. C. Sirard, P. Rothlisberger, M. F. Bachmann, Role of Toll-like receptors in costimulating cytotoxic T cell responses. Eur. J. Immunol. 33, 1465\u20131470 (2003).","journal-title":"Eur. J. Immunol."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1128\/JVI.79.2.955-965.2005","article-title":"Novel adeno-associated virus vector vaccine restricts replication of simian immunodeficiency virus in macaques","volume":"79","author":"Johnson P. R.","year":"2005","unstructured":"P. R. Johnson, B. C. Schnepp, M. J. Connell, D. Rohne, S. Robinson, G. R. Krivulka, C. I. Lord, R. Zinn, D. C. Montefiori, N. L. Letvin, K. R. Clark, Novel adeno-associated virus vector vaccine restricts replication of simian immunodeficiency virus in macaques. J. Virol. 79, 955\u2013965 (2005).","journal-title":"J. Virol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.addr.2016.07.005","article-title":"Virus-inspired nucleic acid delivery system: Linking virus and viral mimicry","volume":"106","author":"Ni R.","year":"2016","unstructured":"R. Ni, J. Zhou, N. Hossain, Y. Chau, Virus-inspired nucleic acid delivery system: Linking virus and viral mimicry. Adv. Drug Deliv. Rev. 106, 3\u201326 (2016).","journal-title":"Adv. Drug Deliv. Rev."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"687","DOI":"10.3389\/fimmu.2017.00687","article-title":"A bivalent anthrax-plague vaccine that can protect against two tier-1 bioterror pathogens, Bacillus anthracis and Yersinia pestis","volume":"8","author":"Tao P.","year":"2017","unstructured":"P. Tao, M. Mahalingam, J. Zhu, M. Moayeri, M. L. Kirtley, E. C. Fitts, J. A. Andersson, W. S. Lawrence, S. H. Leppla, A. K. Chopra, V. B. Rao, A bivalent anthrax-plague vaccine that can protect against two tier-1 bioterror pathogens, Bacillus anthracis and Yersinia pestis. Front. Immunol. 8, 687 (2017).","journal-title":"Front. Immunol."}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.aax0064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T17:18:58Z","timestamp":1704820738000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.aax0064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,2]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8,2]]}},"alternative-id":["10.1126\/sciadv.aax0064"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.aax0064","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,2]]},"article-number":"eaax0064"}}