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The screen reveals Rab10 as a substrate for DENND2B, a protein previously implicated in cancer and severe mental retardation. Through activation of Rab10, DENND2B represses the formation of primary cilia. Through a second pathway, DENND2B functions as a GEF for RhoA to control the length of primary cilia. This work thus identifies an unexpected diversity in DENN domain\u2013mediated activation of Rabs, a previously unidentified non-Rab substrate for a DENN domain, and a new regulatory protein in primary ciliogenesis.<\/jats:p>","DOI":"10.1126\/sciadv.abk3088","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T13:59:33Z","timestamp":1645624773000},"source":"Crossref","is-referenced-by-count":24,"title":["A cell-based GEF assay reveals new substrates for DENN domains and a role for DENND2B in primary ciliogenesis"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7762-807X","authenticated-orcid":true,"given":"Rahul","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Neurology and Neurosurgery, Montreal Neurological Institute (the Neuro), McGill University, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Montreal Neurological Institute (the Neuro), McGill University, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2018-0534","authenticated-orcid":true,"given":"Gopinath","family":"Kulasekaran","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Montreal Neurological Institute (the Neuro), McGill University, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5365-3353","authenticated-orcid":true,"given":"Maleeha","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Montreal Neurological Institute (the Neuro), McGill University, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4613-8817","authenticated-orcid":true,"given":"Gary A. 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