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Inhibition of non-muscle myosin II (NM II)\u2013driven apical constriction altered ISC shape and reduced niche curvature and stem cell capacity. Niche curvature is decreased in aged mice, suggesting that suboptimal interactions between old ISCs and their niche develop with age. We show that activation of NM IIC or physical restriction to young topology improves in vitro regeneration by old epithelium. We propose that the increase in lateral surface area of ISCs induced by apical constriction promotes interactions between neighboring cells, and the curved topology of the intestinal niche has evolved to maximize signaling between ISCs and neighboring cells.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.abm1847","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T14:00:45Z","timestamp":1665756045000},"source":"Crossref","is-referenced-by-count":68,"title":["Cellular shape reinforces niche to stem cell signaling in the small intestine"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3578-1073","authenticated-orcid":true,"given":"Nalle","family":"Pentinmikko","sequence":"first","affiliation":[{"name":"Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, 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