{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:29:38Z","timestamp":1791419378988,"version":"4.3.3"},"reference-count":51,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"27","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2022,7,8]]},"abstract":"<jats:p>\n            Depletion of circulating asparagine with\n            <jats:sc>l<\/jats:sc>\n            -asparaginase (ASNase) is a mainstay of leukemia treatment and is under investigation in many cancers. Expression levels of asparagine synthetase (ASNS), which catalyzes asparagine synthesis, were considered predictive of cancer cell sensitivity to ASNase treatment, a notion recently challenged. Using [U-\n            <jats:sup>13<\/jats:sup>\n            C\n            <jats:sub>5<\/jats:sub>\n            ]-\n            <jats:sc>l<\/jats:sc>\n            -glutamine in vitro and in vivo in a mouse model of B cell lymphomas (BCLs), we demonstrated that supraphysiological or physiological concentrations of asparagine prevent de novo asparagine biosynthesis, regardless of ASNS expression levels. Overexpressing ASNS in ASNase-sensitive BCL was insufficient to confer resistance to ASNase treatment in vivo. Moreover, we showed that ASNase\u2019s glutaminase activity enables its maximal anticancer effect. Together, our results indicate that baseline ASNS expression (low or high) cannot dictate BCL dependence on de novo asparagine biosynthesis and predict BCL sensitivity to dual ASNase activity. Thus, except for ASNS-deficient cancer cells, ASNase\u2019s glutaminase activity should be considered in the clinic.\n          <\/jats:p>","DOI":"10.1126\/sciadv.abn6491","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T17:52:46Z","timestamp":1657129966000},"source":"Crossref","is-referenced-by-count":34,"title":["Tumoral microenvironment prevents de novo asparagine biosynthesis in B cell lymphoma, regardless of ASNS expression"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1875-9870","authenticated-orcid":true,"given":"Manuel","family":"Grima-Reyes","sequence":"first","affiliation":[{"name":"Universit\u00e9 C\u00f4te d\u2019Azur, Inserm, C3M, Nice, France."},{"name":"Equipe labellis\u00e9e Ligue Contre le Cancer, Nice, 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