{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:54:11Z","timestamp":1787316851231,"version":"build-2736575974"},"reference-count":78,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"27","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2022,7,8]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic>Down syndrome cell adhesion molecule 1<\/jats:italic>\n                    (\n                    <jats:italic>Dscam1<\/jats:italic>\n                    ) gene can generate tens of thousands of isoforms via alternative splicing, which is essential for nervous and immune functions. Chelicerates generate approximately 50 to 100 shortened Dscam (sDscam) isoforms by alternative promoters, similar to mammalian protocadherins. Here, we reveal that trans-splicing markedly increases the repository of sDscam\u03b2 isoforms in\n                    <jats:italic>Tetranychus urticae<\/jats:italic>\n                    . Unexpectedly, every variable exon cassette engages in trans-splicing with constant exons from another cluster. Moreover, we provide evidence that competing RNA pairing not only governs alternative cis-splicing but also facilitates trans-splicing. Trans-spliced sDscam isoforms mediate cell adhesion ability but exhibit the same homophilic binding specificity as their cis-spliced counterparts. Thus, we reveal a single\n                    <jats:italic>sDscam<\/jats:italic>\n                    locus that generates diverse adhesion molecules through cis- and trans-splicing coupled with alternative promoters. These findings expand understanding of the mechanism underlying molecular diversity and have implications for the molecular control of neuronal and\/or immune specificity.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.abn9458","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T13:52:46Z","timestamp":1657115566000},"source":"Crossref","is-referenced-by-count":12,"title":["Trans-splicing facilitated by RNA pairing greatly expands sDscam isoform diversity but not homophilic binding specificity"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0685-9775","authenticated-orcid":true,"given":"Shouqing","family":"Hou","sequence":"first","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3135-7040","authenticated-orcid":true,"given":"Guo","family":"Li","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4366-5702","authenticated-orcid":true,"given":"Bingbing","family":"Xu","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2364-6448","authenticated-orcid":true,"given":"Haiyang","family":"Dong","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixin","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Fu","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9019-2154","authenticated-orcid":true,"given":"Jilong","family":"Shi","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. 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China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4654-0622","authenticated-orcid":true,"given":"Yijun","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang ZJ310018, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7125-7055","authenticated-orcid":true,"given":"Yongfeng","family":"Jin","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang ZJ310058, P. R. 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